Mechanisms of anti-ulcer actions of Prangos pabularia (L.) in ethanol-induced gastric ulcer in rats

Jabbar, Ahmed A.J.; Mothana, Ramzi A.; Ameen Abdulla, Mahmood; Othman Abdullah, Fuad; Abdul-Aziz Ahmed, Khaled; Rizgar Hussen, Rawaz; Hawwal, Mohammed F.; Fantoukh, Omer I.; Hasson, Sidgi

doi:10.1016/j.jsps.2023.101850

Cited by 8 publications

(4 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reactive species can have stimulatory action on the inflammatory process and activate the inflammatory cascade, cytokine release, leukocyte infiltration (chemotaxis) and oxidative stress in colon tissues. 12 To prevent such circumstances, colon tissues secret endogenous antioxidants (SOD, CAT and GPx) that balance the free radicals and reduce oxidative damage. However, if the free radical production outrates the antioxidant enzyme secretion, as occurs during certain disease and non-disease conditions, colon tissues become more vulnerable to structural and functional irregularities.…”

Section: Discussionmentioning

confidence: 99%

“… 11 Moreover, inflammation may also result from the pathogenic entrance that leads to up-regulation of the pro-inflammatory cytokine, tumour necrosis factor-alpha (TNF-α), interleukin-6, IL-8, chemokines CCL2, and CXCL8 while reducing anti-inflammatory cytokines (IL-10). 12 Chronic inflammation (CI), a long-term health defect, has been correlated with many of lifelong series diseases namely, irritable bowel disease and colorectal cancer. Irritable bowel disease (IBD) is an inflammatory-related gastrointestinal disease recognized by disruption of intestinal epithelium (intestinal barrier), which usually prevents penetrations of pathogens and toxic compounds and permits passage of only certain micromolecules (nutrients and electrolytes) through different ion and protein channels.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pinostrobin attenuates azoxymethane-induced colorectal cytotoxicity in rats through augmentation of apoptotic Bax/Bcl-2 proteins and antioxidants

Ali Abed Wahab,

Ain Salehen,

Abdulla

et al. 2023

SAGE Open Medicine

View full text Add to dashboard Cite

Objectives: Pinostrobin (5-hydroxy-7-methoxyflavanone; PN) is a natural active ingredient with numerous biological activities extensively utilized in tumour chemotherapy. The present study investigates the chemo-preventive potentials of PN on azoxymethane-mediated colonic aberrant crypt foci in rats. Methods: Sprague Dawley rats clustered into five groups, normal control (A) and cancer controls were subcutaneously injected with normal saline and 15 mg/kg azoxymethane, respectively, and nourished on 10% tween 20 and fed on 10% tween 20; reference control (C), injected with 15 mg/kg azoxymethane and injected (intraperitoneal) with 35 mg/kg 5-fluorouracil (5-FU); D and E rat groups received a subcutaneous injection of 15 mg/kg azoxymethane and nourished on 30 and 60 mg/kg of PN, respectively. Results: The acute toxicity trial showed a lack of any abnormal signs or mortality in rats ingested with 250 and 500 mg/kg of PN. The gross morphology of colon tissues revealed significantly lower total colonic aberrant crypt foci incidence in PN-treated rats than that of cancer controls. Histological examination of colon tissues showed increased aberrant crypt foci availability with bizarrely elongated nuclei, stratified cells and higher depletion of the submucosal glands in cancer controls. PN treatment caused positive modulation of apoptotic (Bax and Bcl-2) proteins and inflammatory cytokines (TNF-α, IL-6 and IL-10). Moreover, rats fed on PN had significantly higher antioxidants (superoxide dismutase) and lower malondialdehyde concentrations in their colon tissue homogenates. Conclusion: The chemoprotective efficiency of PN against azoxymethane-induced aberrant crypt foci is shown by lower aberrant crypt foci values and higher aberrant crypt foci inhibition percentage, possibly through augmentation of genes responsible for apoptotic cascade and inflammations originating from azoxymethane oxidative stress insults.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pinostrobin attenuates azoxymethane-induced colorectal cytotoxicity in rats through augmentation of apoptotic Bax/Bcl-2 proteins and antioxidants

Ali Abed Wahab,

Ain Salehen,

Abdulla

et al. 2023

SAGE Open Medicine

View full text Add to dashboard Cite

show abstract

“…The solution moved into a rotary vacuum evaporator to eliminate the solvent. After freeze‐drying, the extract was kept in a vacuum desiccator for later analysis 27 …”

Section: Methodsmentioning

confidence: 99%

Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms

Ahmed,

Jabbar,

Galali

et al. 2024

Skin Research and Technology

Self Cite

View full text Add to dashboard Cite

Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound healing potentials of methanolic extract of Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on the back of the neck, and received two daily topical treatments for 14 days: A, rats received normal saline; B, wounded rats treated with intrasite gel; C and D, rats received 0.2 mL of 250 and 500 mg/kg of CCS, respectively. After that, wound area and closure percentage were evaluated, and wound tissues were dissected for histopathological, immunohistochemical, and biochemical examinations. Acute toxicity trials of methanolic extract of CCS showed the absence of any physiological changes or mortality in rats. CCS application caused a significant reduction in wound size and a statistically elevated percentage of wound contraction than those of vehicle rats. CCS treatment caused significant up‐regulation of collagen fiber, fibroblasts, and fewer inflammatory cells (inflammation) in granulation tissues. TGF‐β1 (angiogenetic factor) was significantly more expressed in CCS‐treated rats in comparison to normal saline‐treated rats; therefore, more fibroblasts transformed into myofibroblasts (angiogenesis). CCS‐treated rats showed remarkable antioxidant potentials (higher SOD and CAT enzymes) and decreased MDA (lipid peroxidation) levels in their wound tissue homogenates. Hydroxyproline amino acid (collagen) was significantly up‐regulated by CCS treatment, which is commonly related to faster wound closure area. The outcomes suggest CCS as a viable new source of pharmaceuticals for wound treatment.

show abstract

“…Ethanol-induced GU in rats is characterized by the increased production of hydroperoxy free radicals and superoxide anions, leading to lipid peroxidation and oxidative stress in the gastric tissues 4 . In comparison to other GU models, ethanol-induced GU model offers distinct advantage due to close resemblence to the the symptoms observed in acute clinical peptic ulcer disease.…”

Section: Introductionmentioning

confidence: 99%

Ipomoea carnea mitigates ethanol-induced ulcers in irradiated rats via Nrf2/HO−1 pathway: an in vivo and in silico study

Ghareeb,

Mohammed,

Aboushousha

et al. 2024

Sci Rep

View full text Add to dashboard Cite

The aim of this study was to investigate the potential of Ipomoea carnea flower methanolic extract (ICME) as a natural gastroprotective therapy against ethanol-induced gastric ulcers, particularly in individuals exposed to ionizing radiation (IR). The study focused on the Nrf2/HO−1 signaling pathway, which plays a crucial role in protecting the gastrointestinal mucosa from oxidative stress and inflammation. Male Wistar rats were divided into nine groups, the control group received distilled water orally for one week, while other groups were treated with ethanol to induce stomach ulcers, IR exposure, omeprazole, and different doses of ICME in combination with ethanol and/or IR. The study conducted comprehensive analyses, including LC-HRESI-MS/MS, to characterize the phenolic contents of ICME. Additionally, the Nrf2/HO−1 pathway, oxidative stress parameters, gastric pH, and histopathological changes were examined. The results showed that rats treated with IR and/or ethanol exhibited histopathological alterations, increased lipid peroxidation, decreased antioxidant enzyme activity, and reduced expression levels of Nrf2 and HO−1. However, pretreatment with ICME significantly improved these parameters. Phytochemical analysis identified 39 compounds in ICME, with flavonoids, hydroxybenzoic acids, and fatty acids as the predominant compounds. Virtual screening and molecular dynamics simulations suggested that ICME may protect against gastric ulceration by inhibiting oxidative stress and inflammatory mediators. In conclusion, this study demonstrates the potential of ICME as a natural gastroprotective therapy for preventing gastric ulcers. These findings contribute to the development of novel interventions for gastrointestinal disorders using natural plant extracts particularly in individuals with a history of radiation exposure.

show abstract

Mechanisms of anti-ulcer actions of Prangos pabularia (L.) in ethanol-induced gastric ulcer in rats

Cited by 8 publications

References 69 publications

Pinostrobin attenuates azoxymethane-induced colorectal cytotoxicity in rats through augmentation of apoptotic Bax/Bcl-2 proteins and antioxidants

Pinostrobin attenuates azoxymethane-induced colorectal cytotoxicity in rats through augmentation of apoptotic Bax/Bcl-2 proteins and antioxidants

Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms

Ipomoea carnea mitigates ethanol-induced ulcers in irradiated rats via Nrf2/HO−1 pathway: an in vivo and in silico study

Contact Info

Product

Resources

About