Mechanisms of action of non-enzymatic antioxidants to control oxidative stress during in vitro follicle growth, oocyte maturation, and embryo development

Silva, Bianca R.; Silva, José R.V.

doi:10.1016/j.anireprosci.2022.107186

Cited by 19 publications

(12 citation statements)

References 169 publications

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“…The addition of antioxidants to oocyte culture in vitro has been shown to exert a positive effect, improving oocyte maturation rates by attenuating oxidative stress and restoring impaired mitochondrial performance. In the present study, the addition of GC-EXs reduced ROS levels in oocytes, increased the mitochondrial membrane potential, and optimized antioxidant capacity [ 66 , 67 , 68 ]. As a result, the developmental level and quality of oocytes significantly improved.…”

Section: Discussionmentioning

confidence: 99%

Porcine Granulosa-Cell-Derived Exosomes Enhance Oocyte Development: An In Vitro Study

Ren,

Ding,

Shi

et al. 2024

Antioxidants

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Recent studies have established that exosomes (EXs) derived from follicular fluid (FF) can promote oocyte development. However, the specific sources of these EXs and their regulatory mechanisms remain elusive. It is universally acknowledged that oocyte development requires signal communication between granulosa cells (GCs) and oocytes. However, the role of GC-secreted EXs and their functions are poorly understood. This study aimed to investigate the role of porcine granulosa-cell-derived exosomes (GC-EXs) in oocyte development. In this study, we constructed an in vitro model of porcine GCs and collected and identified GC-EXs. We confirmed that porcine GCs can secrete EXs and investigated the role of GC-EXs in regulating oocyte development by supplementing them to cumulus–oocyte complexes (COCs) cultured in vitro. Specifically, GC-EXs increase the cumulus expansion index (CEI), promote the expansion of the cumulus, alleviate reactive oxygen species (ROS), and increase mitochondrial membrane potential (MMP), resulting in improved oocyte development. Additionally, we conducted small RNA sequencing of GC-EXs and hypothesized that miR-148a-3p, the highest-expressed microRNA (miRNA), may be the key miRNA. Our study determined that transfection of miR-148a-3p mimics exerts effects comparable to the addition of EXs. Meanwhile, bioinformatics prediction, dual luciferase reporter gene assay, and RT-qPCR identified DOCK6 as the target gene of miR-148a-3p. In summary, our results demonstrated that GC-EXs may improve oocyte antioxidant capacity and promote oocyte development through miR-148a-3p by targeting DOCK6.

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Section: Discussionmentioning

confidence: 99%

Porcine Granulosa-Cell-Derived Exosomes Enhance Oocyte Development: An In Vitro Study

Ren,

Ding,

Shi

et al. 2024

Antioxidants

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“…Over the past two decades, many studies have been conducted regarding the beneficial power of Res on health [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. Among others, Res appears to have a role associated with slowing down or preventing cognitive impairment.…”

Section: Discussionmentioning

confidence: 99%

A Novel Resveratrol-Induced Pathway Increases Neuron-Derived Cell Resilience against Oxidative Stress

Cracco

Montalesi

Parente

et al. 2023

IJMS

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A promising therapeutic strategy to delay and/or prevent the onset of neurodegenerative diseases (NDs) could be to restore neuroprotective pathways physiologically triggered by neurons against stress injury. Recently, we identified the accumulation of neuroglobin (NGB) in neuronal cells, induced by the 17β-estradiol (E2)/estrogen receptor β (ERβ) axis, as a protective response that increases mitochondria functionality and prevents the activation of apoptosis, increasing neuron resilience against oxidative stress. Here, we would verify if resveratrol (Res), an ERβ ligand, could reactivate NGB accumulation and its protective effects against oxidative stress in neuronal-derived cells (i.e., SH-SY5Y cells). Our results demonstrate that ERβ/NGB is a novel pathway triggered by low Res concentrations that lead to rapid and persistent NGB accumulation in the cytosol and in mitochondria, where the protein contributes to reducing the apoptotic death induced by hydrogen peroxide (H2O2). Intriguingly, Res conjugation with gold nanoparticles increases the stilbene efficacy in enhancing neuron resilience against oxidative stress. As a whole, ERβ/NGB axis regulation is a novel mechanism triggered by low concentration of Res to regulate, specifically, the neuronal cell resilience against oxidative stress reducing the triggering of the apoptotic cascade.

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“…Excessive production of ROS can lead to oxidative stress and damage to oocytes, which may impair their maturation. For example, ROS can damage the DNA of germ cells and affect their repair functions, leading to cellular dysfunction (22). At the same time, ROS can also suppress signaling within germ cells, affecting their maturation.…”

Section: Discussionmentioning

confidence: 99%

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

Yang

Duan

et al. 2023

Preprint

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Background Brilliant cresyl blue (BCB) staining can stain oocytes and differentiated oocytes will lead to different developmental outcomes. This technique has been studied in multiple species, but it is still unclear whether buffalo oocytes can be used for developmental potential prediction through BCB staining methods. This study used the BCB staining method to group buffalo oocytes (BCB + and BCB-) and perform in vitro maturation, in vitro fertilization, and embryo culture. By statistical analysis, the effect of BCB staining on predicting the developmental potential of buffalo oocytes will be explored. At the same time, molecular biology techniques will be used to detect gap junction protein and oxidative stress-related indicators to explore the molecular mechanism of BCB staining predicting oocyte cell developmental potential. Methods The oocytes were divided into BCB + and BCB- groups using BCB staining technique. And then mainly uses in vitro maturation, in vitro fertilization and embryo culture techniques of buffalo oocytes to analyze their developmental potential, and uses immunofluorescence staining to detect the expression level of CX43 protein, DCFH-DA probe staining to detect ROS levels, and qPCR to detect the expression levels of the antioxidant related genes SOD2 and GPX1. Results Our results showed that the in vitro maturation rate, embryo cleavage rate, and blastocyst rate of buffalo oocytes in the BCB + group were significantly higher than those in the BCB- group and the control group (P < 0.05). The expression level of CX43 protein in the BCB + group was higher than that in the BCB- group both before and after maturation (P < 0.05). The intensity of ROS in the BCB + group was significantly lower than that in the BCB- group (P < 0.05), and the expression levels of the antioxidant-related genes SOD2 and GPX1 in the BCB + group were significantly higher than those in the BCB- group (P < 0.05). Conclusions BCB staining can effectively predict the developmental potential of buffalo oocytes. The results of BCB staining are positively correlated with the expression of gap junction protein and antioxidant-related genes and negatively correlated with the ROS level, suggesting that the mechanism of BCB staining in predicting the developmental potential of buffalo oocytes may be closely related to antioxidant activity.

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Mechanisms of action of non-enzymatic antioxidants to control oxidative stress during in vitro follicle growth, oocyte maturation, and embryo development

Cited by 19 publications

References 169 publications

Porcine Granulosa-Cell-Derived Exosomes Enhance Oocyte Development: An In Vitro Study

Porcine Granulosa-Cell-Derived Exosomes Enhance Oocyte Development: An In Vitro Study

A Novel Resveratrol-Induced Pathway Increases Neuron-Derived Cell Resilience against Oxidative Stress

CX43 and oxidative stress are the targets of BCB staining to predict the developmental potential of buffalo oocytes

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