Mechanisms of Action of Luteolin Against Single- and Dual-Species of Escherichia coli and Enterobacter cloacae and Its Antibiofilm Activities

Qian, Weidong; Fu, Yuting; Liu, Miao; Zhang, Jianing; Wang, Wenjing; Li, Jingyuan; Zeng, Qiao; Wang, Ting

doi:10.1007/s12010-020-03330-w

Cited by 24 publications

(19 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wouamba et al [57] reported a low MIC of 3.12 µg/mL against E. coli and 6.25 µg/mL against S. Typhimurium; however, only one reference strain per each bacterial species was tested in their study. MIC values of 64 µg/mL were obtained by Qian et al [58] when they tested the activity of LUT against four strains of E. coli, including one ATCC strain and three isolates from raw pork meat. Adamczak et al [59] have also investigated antibacterial effect of LUT against four E. coli strains, but they could only find inhibitory activity at higher concentrations (MIC: 500 µg/mL).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity of Flavonoids in LPS-Treated IPEC-J2 Porcine Intestinal Epithelial Cells and Their Antibacterial Effect against Bacteria of Swine Origin

et al. 2020

View full text Add to dashboard Cite

Beneficial effects of flavonoids are widely known in human medicine, but less information is available about their veterinary usage. Based on their antioxidant and antibacterial activity, proanthocyanidins (PAs) and luteolin (LUT) might be used in the prevention and treatment of gastrointestinal infections in swine. In this study, in vitro beneficial effects of grape seed oligomeric proanthocyanidins (GSOPs) and LUT were investigated against bacterial endotoxin (LPS)-induced oxidative stress in IPEC-J2 porcine epithelial intestinal cells. Furthermore, antibacterial effects of GSOP and LUT were assessed against field isolates of Escherichia coli and Salmonella enterica ser. Typhimurium. Both GSOP and LUT were found to possess potent in vitro antioxidant activity in LPS-treated IPEC-J2 cells; furthermore, they showed a bacteriostatic effect against the tested bacterial strains of porcine origin. Both flavonoids seem to be effective in the protection of porcine intestinal epithelial cells against Gram-negative bacteria in vitro, but further in vivo studies are necessary to confirm these activities and to establish their optimal dosage regimen for future usage in veterinary practice.

show abstract

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity of Flavonoids in LPS-Treated IPEC-J2 Porcine Intestinal Epithelial Cells and Their Antibacterial Effect against Bacteria of Swine Origin

et al. 2020

View full text Add to dashboard Cite

show abstract

“…According to Schwering et al [53], dual-species biofilms of E. coli and E. cloacae are up to 300 times more resistant to chlorine than single-species biofilms. Furthermore, Qian et al [49] investigated the effect of luteolin on mixed biofilm formed by E. coli ATCC 25922 and E. cloacae ATCC 13047. Qian et al [49] found that luteolin had a much weaker effect than The results obtained by other authors indicate that different antibacterial agents show better activity against single-species biofilms than multi-species biofilms.…”

Section: The P Aeruginosa Cell Count In Mono- Dual- and Triple-species Biofilms Treated With Aamentioning

confidence: 99%

“…Furthermore, Qian et al [49] investigated the effect of luteolin on mixed biofilm formed by E. coli ATCC 25922 and E. cloacae ATCC 13047. Qian et al [49] found that luteolin had a much weaker effect than The results obtained by other authors indicate that different antibacterial agents show better activity against single-species biofilms than multi-species biofilms. The results of this study, however, are difficult to discuss because they are novel.…”

Section: The P Aeruginosa Cell Count In Mono- Dual- and Triple-species Biofilms Treated With Aamentioning

confidence: 99%

“…Furthermore, Qian et al [49] investigated the effect of luteolin on mixed biofilm formed by E. coli ATCC 25922 and E. cloacae ATCC 13047. Qian et al [49] found that luteolin had a much weaker effect than when the bacteria were grown in monocultures. The data presented in Figure 13 shows that AA significantly inhibited the synthesis of biofilm formed by the rods growing in mono-species consortia (p ≤ 0.05).…”

Section: The P Aeruginosa Cell Count In Mono- Dual- and Triple-species Biofilms Treated With Aamentioning

confidence: 99%

See 1 more Smart Citation

Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?

et al. 2021

View full text Add to dashboard Cite

Urinary tract infections (UTIs) are a serious health problem in the human population due to their chronic and recurrent nature. Bacteria causing UTIs form multispecies biofilms being resistant to the activity of the conventionally used antibiotics. Therefore, compounds of plant origin are currently being searched for, which could constitute an alternative strategy to antibiotic therapy. Our study aimed to determine the activity of asiatic acid (AA) against biofilms formed by uropathogenic Escherichia coli, Enterobacter cloacae, and Pseudomonas aeruginosa. The influence of AA on the survival, biofilm mass formation by bacteria living in mono-, dual-, and triple-species consortia as well as the metabolic activity and bacterial cell morphology were determined. The spectrophotometric methods were used for biofilm mass synthesis and metabolic activity determination. The survival of bacteria was established using the serial dilution assay. The decrease in survival and a weakening of the ability to create biofilms, both single and multi-species, as well as changes in the morphology of bacterial cells were noticed. As AA works best against young biofilms, the use of AA-containing formulations, especially during the initial stages of infection, seems to be reasonable. However, there is a need for further research concerning AA especially regarding its antibacterial mechanisms of action.

show abstract

“…Due to their wide range of sources and low toxicity, medicinal plant-derived native compounds are attracting great public interest. Luteolin (3',4',5,7-tetrahydroxyflavone), a natural flavonoid rich in a variety of plant products, exhibits antimicrobial activity by damaging bacterial cell membranes, cell walls and biofilms [25][26][27][28]. The main strategies by which luteolin affects bacterial proteins include binding to or interacting with proteins, decreasing the secretion of proteins, and inhibiting protein expression [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

A Functional Study of the TatD DNases of <em>Trueperella pyogenes</em> and the Conformational Analysis of Luteolin as an Inhibitor

Zhang¹,

Liang²,

Yu³

et al. 2021

Preprint

View full text Add to dashboard Cite

Trueperella pyogenes (T. pyogenes) can cause a variety of infections in animals and lead to economic loss in animal husbandry. TatD DNases are considered to be potential virulence factors in Plasmodium falciparum and Streptococcus pneumoniae. However, the function of TatD DNases in T. pyogenes is still unclear. Therefore, the aim of this study was to illustrate the function of TatD DNases of T. pyogenes (TpTatDs) and investigate whether luteolin is able to inhibit DNase function. The findings of our study are anticipated to be crucial to treating infections caused by T. pyogenes. Bioinformatic analysis has been used for the prediction of crucial functional residues of TpTatDs. The function of TpTatDs was investigated in the presence of divalent cations by hydrolyzing DNA with recombinant TatD proteins. Luteolin is a candidate nuclease inhibitor evaluated in our study. The interactions between luteolin and TpTatDs were tested using molecular docking analysis and surface plasmon resonance (SPR) assays. The inhibitory effect of luteolin on TpTatDs was analyzed by agarose gel electrophoresis. Two genes in the genome of T. pyogenes are suspected to encode TatD DNases. According to the length of their nucleotide sequences, they were named tatD960 and tatD825. Both of the TpTatDs, which are magnesium-dependent, were able to hydrolyze linear DNA and plasmids. In this study, we found through molecular docking analysis and SPR assays that luteolin can stably bind with TpTatDs. The gel assay revealed that luteolin can inhibit the DNase activity of TpTatDs. Our results indicated that TatD DNases from T. pyogenes are Mg2+-dependent DNases and exhibit DNA endonuclease activity. Moreover, luteolin reduced their DNA hydrolysis ability by decreasing the binding between TpTatDs and DNA.

show abstract

Mechanisms of Action of Luteolin Against Single- and Dual-Species of Escherichia coli and Enterobacter cloacae and Its Antibiofilm Activities

Cited by 24 publications

References 29 publications

Antioxidant Activity of Flavonoids in LPS-Treated IPEC-J2 Porcine Intestinal Epithelial Cells and Their Antibacterial Effect against Bacteria of Swine Origin

Antioxidant Activity of Flavonoids in LPS-Treated IPEC-J2 Porcine Intestinal Epithelial Cells and Their Antibacterial Effect against Bacteria of Swine Origin

Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?

A Functional Study of the TatD DNases of <em>Trueperella pyogenes</em> and the Conformational Analysis of Luteolin as an Inhibitor

Contact Info

Product

Resources

About