Mechanisms of action of hydrogen sulfide in relaxation of mouse distal colonic smooth muscle

Dhaese, Ingeborg; Colen, Inge Van; Lefebvre, Romain

doi:10.1016/j.ejphar.2009.11.024

Cited by 51 publications

(47 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…EC 50 values estimated from the inhibitory effect of H 2 S appeared to be ~400mmoll -1 . Relaxation of the mouse distal colon was observed at 100mmoll -1 H 2 S, although this was the lowest concentration tested, and the EC 50 was 444mmoll -1 (Dhaese et al, 2010). In longitudinal muscle from rat ileum, 10mmoll -1 H 2 S reduced AUC, average amplitude of spontaneous activity and baseline tone by 7%, 3% and 6%, respectively (Nagao et al, 2010).…”

Section: Comparison Of H 2 S Effects In Mammals and Troutmentioning

confidence: 99%

“…H 2 S directly relaxes GI smooth muscle in the mouse stomach and colon (Dhaese and Lefebvre, 2009;Dhaese et al, 2010) and rat ileum (Nagao et al, 2010). In the guinea-pig stomach, 'low doses' of H 2 S (0.1-0.3mmoll -1 ) enhance resting tension and slightly reduce contractile amplitude, whereas at higher concentrations H 2 S suppresses the amplitude of spontaneous contractions (Zhao et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The mechanism of H 2 S-mediated relaxation is poorly understood and most common mediators of muscle relaxation have in fact been ruled out. In the mouse, H 2 S-induced relaxations are independent of potassium (K + ) channels, NO, cAMP and cGMP pathways, and nerve inhibition, and do not involve membrane H 2 S and GI motility in salmonids ATPase, calcium (Ca 2+ ) channels, internal calcium stores or Rhokinase; H 2 S may, or may not, act via activation of myosin light chain phosphatase (Dhaese and Lefebvre, 2009;Dhaese et al, 2010). In the rat ileum, H 2 S-mediated relaxations are independent of intrinsic, enteric and visceral afferent nerves, NO and both ATP-(K ATP ) and calcium-sensitive (K Ca ) potassium channels (Nagao et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydrogen sulfide (H2S) and hypoxia inhibit salmonid gastrointestinal motility: evidence for H2S as an oxygen sensor

Dombkowski

Naylor

Shoemaker

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYHydrogen sulfide (H 2 S) has been shown to affect gastrointestinal (GI) motility and signaling in mammals and O 2 -dependent H 2 S metabolism has been proposed to serve as an O 2 ʻsensorʼ that couples hypoxic stimuli to effector responses in a variety of other O 2 -sensing tissues. The low P O2 values and high H 2 S concentrations routinely encountered in the GI tract suggest that H 2 S might also be involved in hypoxic responses in these tissues. In the present study we examined the effect of H 2 S on stomach, esophagus, gallbladder and intestinal motility in the rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch) and we evaluated the potential for H 2 S in oxygen sensing by examining GI responses to hypoxia in the presence of known inhibitors of H 2 S biosynthesis and by adding the sulfide donor cysteine (Cys). We also measured H 2 S production by intestinal tissue in real time and in the presence and absence of oxygen. In tissues exhibiting spontaneous contractions, H 2 S inhibited contraction magnitude (area under the curve and amplitude) and frequency, and in all tissues it reduced baseline tension in a concentration-dependent relationship. Longitudinal intestinal smooth muscle was significantly more sensitive to H 2 S than other tissues, exhibiting significant inhibitory responses at 1-10mmoll -1 H 2 S. The effects of hypoxia were essentially identical to those of H 2 S in longitudinal and circular intestinal smooth muscle; of special note was a unique transient stimulatory effect upon application of both hypoxia and H 2 S. Inhibitors of enzymes implicated in H 2 S biosynthesis (cystathionine -synthase and cystathionine -lyase) partially inhibited the effects of hypoxia whereas the hypoxic effects were augmented by the sulfide donor Cys. Furthermore, tissue production of H 2 S was inversely related to O 2 ; addition of Cys to intestinal tissue homogenate stimulated H 2 S production when the tissue was gassed with 100% nitrogen (~0% O 2 ), whereas addition of oxygen (~10% O 2 ) reversed this to net H 2 S consumption. This study shows that the inhibitory effects of H 2 S on the GI tract of a non-mammalian vertebrate are identical to those reported in mammals and they provide further evidence that H 2 S is a key mediator of the hypoxic response in a variety of O 2 -sensitive tissues.

show abstract

Section: Comparison Of H 2 S Effects In Mammals and Troutmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrogen sulfide (H2S) and hypoxia inhibit salmonid gastrointestinal motility: evidence for H2S as an oxygen sensor

Dombkowski

Naylor

Shoemaker

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…In the mouse colon, it has demonstrated that NaHS inhibited the spontaneous and pre-contracted smooth muscle contraction in a dosedependent manner, which was not via neuronal pathway because both TTX and TRPV1 blocker capsazepine had no significant effects on the inhibition [45,49] . Since glibenclamide and L-NAME, a nitric oxide synthase, did not affect the NaHS-induced inhibition, so that KATP channel and NO were not involved in the effect [49] .…”

Section: In the Colonmentioning

confidence: 99%

“…Since glibenclamide and L-NAME, a nitric oxide synthase, did not affect the NaHS-induced inhibition, so that KATP channel and NO were not involved in the effect [49] . In the rat and human colon, NaHS also inhibited the colonic motility [45,50,51] .…”

Section: In the Colonmentioning

confidence: 99%

Synthesis of H2S in the Gastrointestinal Tract

Huang

2015

Journal of Gastroenterology and Hepatology Research

View full text Add to dashboard Cite

Emerging role of hydrogen sulfide in colonic physiology and pathophysiology

Medani

Collins

Docherty

et al. 2011

Inflammatory Bowel Diseases

128

View full text Add to dashboard Cite

Hydrogen sulfide (H₂S) is a toxic gas that is now recognized as an important mediator of many physiological processes. In the colon, H₂S is produced both endogenously and by naturally occurring sulfate-reducing bacteria (SRB). The full arrays of its effects in the gastrointestinal tract are still being elucidated, but they range from motility to carcinogenesis. We examined the evidence relating to H₂S as a modulator of colonic function and disease. H₂S is implicated in modulation of colonic compliance through its action on smooth muscle. There is also evidence linking H₂S to colonic nociception, inflammatory bowel disease (IBD), and colorectal cancer. The exact mechanisms and pathways by which H₂S exerts its multitude of effects are not yet fully understood, but its involvement in physiological and pathophysiological conditions of the colon is becoming evident. Elucidating the intricate effects of H₂S in the colon and understanding the exact nature of its interactions with the colon makes pharmacological modulation of H₂S production and metabolism potential targets for treatment of a multitude of colonic conditions in the future.

show abstract

Mechanisms of action of hydrogen sulfide in relaxation of mouse distal colonic smooth muscle

Cited by 51 publications

References 39 publications

Hydrogen sulfide (H2S) and hypoxia inhibit salmonid gastrointestinal motility: evidence for H2S as an oxygen sensor

Hydrogen sulfide (H2S) and hypoxia inhibit salmonid gastrointestinal motility: evidence for H2S as an oxygen sensor

Synthesis of H2S in the Gastrointestinal Tract

Emerging role of hydrogen sulfide in colonic physiology and pathophysiology

Contact Info

Product

Resources

About