Mechanisms of action for light therapy: A review of molecular interactions

Prindeze, Nicholas J.; Moffatt, Lauren T.; Shupp, Jeffrey W.

doi:10.1258/ebm.2012.012180

Cited by 76 publications

(55 citation statements)

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“…31,32 It was demonstrated that laser and LED light have similar improvement impact on cells and tissues. Studies have shown that the healing influences of photomedicine are due to transformation of absorbed energy into ATP.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

et al. 2017

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Introduction: In recent years, low-power lasers have been widely used in medicine. With the introduction of affordable light emitted diode (LED), clinical application of LED light has become more and more popular. However, some researchers believe that due to lack of coherence of the LED light, it can be different in terms of biological effects, in comparison with laser. In this study, the biological effects of low-level laser (LLL) to those of LED light are compared and discussed. Methods: Human skin fibroblast cell line Hu02 was irradiated with LLL and LED light with a wavelength of 660 nm, power output of 35 mW and in continuous mode and the control group was not irradiated. The biological effects were compared through analysis of cell proliferation, production of reactive oxygen species (ROS) within the cell and rate of cell division. Results: Our findings showed that production of ROS within the cell was linearly increased both in the LED and laser light irradiated cells. However, laser light is more incremental in comparison to LED light. The MTT results showed that laser light at low energy density (less than 5 J/cm 2 ) increased the rate of cell proliferation after 24 hours. Although, the rate of cell division was increased in energy density of 1 J/cm 2 compared to the control group, this increase was not statistically significant. Discussion: The findings indicated that the coherence properties of laser light provided more energy for the cells, and in a constant energy density, laser light created more oxidative stresses in comparison with LED light.

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Section: Discussionmentioning

confidence: 99%

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

et al. 2017

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“…To reduce pulp damage or improve healing of already damaged pulp tissue, different techniques, such as phototherapy, have been assessed and recommended (3,4). Low-level light therapy (LLLT), with lasers or light-emitting diodes (LED), has been widely used in dentistry for tissue and cell stimulation (5)(6)(7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

et al. 2015

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Despite several reports regarding tissue regeneration, including pulp repair induced by different light sources, only limited data have been reported concerning the effects of light-emitting diodes (LED) on stem cells from human exfoliated deciduous teeth (SHEDs). The aim of this study was to evaluate the effects of different energy densities of infrared LED on the cell viability, number of cells and mineralized tissue production by SHEDs. SHEDs were obtained from near-exfoliation primary teeth (n=3), seeded in plain DMEM (10 4 cells/cm 2 ), and irradiated by a LED prototype (LEDTable 850 nm, 40 mW/cm 2 ) delivering 0 (control), 2, 4, 8, 15 or 30 J/cm 2 (n=9). Cell viability (MTT assay), cell proliferation (trypan blue assay), and mineralized nodule (MN) formation (alizarin red stain) were assessed 12 and 72 h post-irradiation. Data were subjected to Kruskal-Wallis and Mann-Whitney tests (α=0.05). Cells irradiated with 2 or 4 J/cm 2 exhibited higher metabolism at 72 h, and all energy densities provided increase in cell proliferation after 12 h. Regarding MN formation, the best results were observed at 72 h after SHED irradiation with 8 and 15 J/cm 2 . It was concluded that the cell viability, cell number and MN formation by pulp cells are enhanced after exposure to infrared LED irradiation. Overall, the greatest SHED biostimulation was obtained with 4 and 8 J/cm 2 . D o s e -r e s p o n s e s o f S t e m C e l l s f r o m H u m a n E x f o l i a t e d Te e t h t o I n f r a r e d L E D I r r a d i a t i o n

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“…Terapia światłem małych mocy wpływa na wszystkie trzy fazy gojenia ran: fazę zapalną, w której komórki zapalne migrują do rany, fazę proliferacyjną z pobudzeniem fibroblastów i makrofagów oraz fazę dojrzewania, w której nastę-puje proces przebudowy zagojonej już rany, aby stała się bardziej wytrzymała. Pod wpływem właściwie dobranego promieniowania świetlnego następuje zwiększenie produkcji oraz aktywacji fibroblastów i makrofagów, pobudzenie leukocytów i przyspieszenie tworzenia kolagenu oraz neowaskularyzacji [13,19]. Rany, w których przy stosowaniu klasycznych metod leczniczych proces gojenia nie przebiegał właściwie, ulegały zabliźnieniu po zastosowaniu naświetlania diodami LED emitującymi promieniowanie z zakresu widzialnego i bliskiej podczerwieni.…”

Section: Diody Elektroluminescencyjne Ledunclassified

Light-emitting diodes in dermatology: stimulation of wound healing

Fryc¹

2016

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StreSzczenieTerapia światłem o małej mocy, określana skrótem LLLT (ang. low-level light therapy), niekiedy nazywana również fototerapią, jest skuteczną strategią stosowaną w gojeniu ran oraz w celu redukcji dolegliwości bólowych, zapalenia i obrzęku tkanek. W fototerapii coraz powszechniej używa się nowego rodzaju źródła światła, którym jest dioda elektroluminescencyjna (LED). Efekty biologiczne związane z działaniem promieniowania emitowanego przez diody LED są w głównej mierze uzależnione od długości fali i dawki. W pracy przedstawiono przegląd wyników badań dotyczących stymulacji gojenia ran z użyciem terapii światłem LED o małej mocy oraz podkreślono wagę doboru optymalnych parametrów LLLT do tego celu. Omówiono także mechanizmy działania LLLT na poziomie komórkowym i tkankowym. AbStrActLow-level light therapy (LLLT), which is sometimes included in phototherapy, is an effective therapeutic strategy to improve wound healing and reduce pain, inflammation and swelling. Nowadays, new sources of light, such as light-emitting diodes (LEDs) with a broad range of wavelengths, are widely available. The biological effects promoted by LEDs are dependent on irradiation parameters, mainly wavelength and dose. This review article focuses on recent clinical trials using light-emitting diode low-level light therapy (LED-LLLT) for enhancing wound healing. In this article, we also cover the mechanisms of action of LLLT on cells and tissues and highlight the importance of defining optimum LLLT parameters for stimulation of wound healing.

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Mechanisms of action for light therapy: A review of molecular interactions

Cited by 76 publications

References 76 publications

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

Light-emitting diodes in dermatology: stimulation of wound healing

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