Mechanisms involved in α6β1-integrin-mediated Ca2+ signalling

Integrin regulation of cytoplasmic calcium in excitatory neurons depends upon glutamate receptors and release from intracellular stores

“…The pattern of ligand-induced calcium influx followed by intracellular store release is typical of integrin effects on [Ca 2+ ]i in non-neuronal cells (Bhattacharya et al, 2000;Kwon et al, 2000;Schottelndreier et al, 2001). Moreover, when examined, influx is generally found to be mediated by VSCCs and dependent upon tyrosine kinase signaling (i.e., blocked by genistein) (Wu et al, 1998;Kwon et al, 2000;Wu et al, 2001).…”

“…Integrin ligand induced increases in [Ca 2+ ]i are blocked by the broad spectrum tyrosine kinase inhibitor genistein (Xie et al, 1998;Schottelndreier et al, 1999;Wu et al, 2001) as well as by the Src family kinase inhibitor PP2 (Schottelndreier et al, 2001;Wu et al, 2001) in non-neural cells. Other work has shown that integrin signaling activates kinases (CamKII, Src, MAPK p42/44) that modulate glutamate neurotransmitter receptor and VSCC function in neurons Bernard-Trifilo et al, 2005;Watson et al, 2007) and receptor tyrosine kinases in other systems (Miranti and Brugge, 2002).…”

“…Different integrins regulate intracellular calcium levels in non-neuronal cells through effects on influx and release from intracellar stores (Sjaastad et al, 1994;Tsao and Mousa, 1995;Schottelndreier et al, 2001;Wu et al, 2001). The few studies of neurons show that integrin ligands increase calcium influx through effects on voltage-sensitive calcium channels (VSCCs) (Wildering et al, 2002;Gui et al, 2006) and NMDA receptors (Watson et al, 2007), effects that could have a profound influence on the calcium-dependent LTP induction process (Lynch et al, 1983;Rose and Konnerth, 2001).…”

FM-test: A Fuzzy Set Theory Based Approach for Discovering Diabetes Genes

“…It has been shown that during the differentiation of 3T3-L1 fibroblasts to adipocytes, the rate of lipid biosynthesis from leucine increases at least 30-fold and the specific activity of 3-hydroxy-3-methylglutaryl-CoA lyase (gene L07033), the mitochondrial enzyme catalyzing the terminal reaction in the leucine degradation pathway, increases 4-fold during differentiation [7]. Schottelndreier et al [12] have described a regulatory role of integrin alpha 6 (gene ¿ ) in Ca2+ signaling, that is known to have a significant role in insulin resistance [9]. HCGV gene product (gene ½¼¼¿) is known to inhibit the activity of protein phosphatase-1, which is involved in diverse signaling pathways including insulin signaling [18].…”

Knock-down of the Type 3 Ryanodine Receptor Impairs Sustained Ca2+ Signaling via the T Cell Receptor/CD3 Complex

“…In addition to IP 3 and cADPR, nicotinic acid adenine dinucleotide phosphate (NAADP) is an essential regulator of T cell Ca 2ϩ signaling (4), although the exact role of this nucleotide has not yet been clarified. Jurkat T cells preincubated with the specific, membrane-permeant cADPR antagonist 7-deaza-8-Br-cADPR (5) showed characteristic defects in the onset and in the longlasting phase of TCR/CD3-and ␤ 1 -integrin-mediated Ca 2ϩ signaling (2,6). In addition, in peripheral human T cells, 7-deaza-8-Br-cADPR efficiently blocked expression of the activation antigens CD25 and MHCII and blocked proliferation upon CD3 ligation (2).…”