Mechanisms Involved in Milk Endogenous Proteolysis Induced by a Lipopolysaccharide Experimental Mastitis

Moussaoui, F.; Michelutti, I.; Roux, Yves Le; Laurent, François

doi:10.3168/jds.s0022-0302(02)74339-7

Cited by 29 publications

(54 citation statements)

References 32 publications

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“…Our results are also consistent with previous studies showing that LPS challenge induces significant casein degradation and identifies milk PL as a major cause for this (Moussaoui et al, 2002;Leitner et al, 2004aLeitner et al, , 2004bLeitner et al, and 2004cSilanikove et al, 2006;Rossi et al, 2009). The biological implications of these findings have been discussed before Silanikove et al, 2006) and, therefore, these aspects are not discussed here in detail.…”

Section: Lps Challenge Is Reflected By Accumulation Of Inflammatory Isupporting

confidence: 93%

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Silanikove

Rauch-Cohen

Shapiro

et al. 2012

Animal

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The aim of this work was to study the effects of mastitis induced by intramammary lipopolysaccharide (LPS) challenge on milk oxidative stability, as well as to understand the underlying biochemical processes that cause such changes. LPS challenge was associated with nitric oxide burst from the surrounding mammary epithelial cells and consequently induced nitrosative stress that was induced by the formation of NO 2 d from nitrite by lactoperoxidase. This response was associated with an , 3-fold increased formation of hazardous compounds: nitrotyrosines, carbonyls and lipid peroxides. We sustained the involvement of xanthine oxidase as a major source of hydrogen peroxide. In consistent with previous findings, catalase has been shown to play a major role in modulating the nitrosative stress by oxidizing nitrite to nitrate. The current hygienic quality criteria cannot detect mixing of low-quality milk, such as milk with high somatic cells, and nitrite with high-quality milk. Thus, development of an improved quality control methodology may be important for the production of high-quality milk.Keywords: milk, cow, mastitis, NO and NO 2 d, plasmin system Implication In this study, we show that intramammary treatment with lipopolysaccharide induced nitric oxide burst that was associated with nitrosative stress that reflected the formation of hazardous compounds in milk: nitrotyrosines, carbonyls and lipid peroxides. These changes were associated with impairment in milk quality. Currently, very little is known about the presence of oxidative substances in commercial milk and their industrial and health implications. As mastitis is a prevalent problem in the dairy industry, this study stresses the importance of gaining further information on the effect of different forms of mastitis on milk composition.

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Section: Lps Challenge Is Reflected By Accumulation Of Inflammatory Isupporting

confidence: 93%

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Silanikove

Rauch-Cohen

Shapiro

et al. 2012

Animal

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“…[31,54]. After LPS infusion, the capacity of caseinolysis of PMN neutral proteases and acidic proteases has been shown by Moussaoui et al [50]. PMN are reported to contain acidic proteases such as cathepsins B, C, D or L [54].…”

Section: Caseinolysis By Pmnmentioning

confidence: 99%

“…Prostaglandin E2 (PGE2) has a stimulating effect on u-PA and t-PA [9], concomitantly PGE2 concentration increases during mastitis [80]. Serine proteases stimulate the action of plasminogen activators which is as important as the increase of plasmin activity (+570%, [50]), cathepsin G from milk PMN (+542%, [60]) and elastase activities (+164%, [60]). In addition, plasmin activates Collagenase IV by cleaving its zymogen [54].…”

Section: Plasmin Activity (Tab Vi)mentioning

confidence: 99%

Polymorphonuclear proteolytic activity and milk composition change

Roux¹,

Laurent²,

Moussaoui³

2003

Vet. Res.

114

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-Relationships between Quarter Milk Cell Count (QMCC) and Tank Milk Cell Count (TMCC) with milk biochemical and technological parameters in milk and dairy products were investigated. All parameters measured were affected by the increase of TMCC and QMCC between 0 and 600 000 cells/mL. The variable effect of lactation stage which is different for different authors, is discussed. The three mechanisms, measured during the inflammation of the udder, implicated in the modification of milk quality are described (a decrease in synthesis, a decrease in the milk barrier permeability and an increase in proteolytic activities). The direct effect of plasmin in caseinolysis is well known; the specific role of the increase of somatic cells (especially PMN) in the modification of milk quality is described. Several specific proteolytic activities of PMN are described and the impact of these activities on caseinolysis is evaluated. Two hypothetical mechanisms of caseinolysis by PMN are suggested and a synthetic scheme of the role of plasmin, bacteria and somatic cells in caseinolysis is discussed. caseinolysis / mastitis / milk / PMN proteases / SCC

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“…Proteose-peptones (PP), somatic cell count (SCC) and plasmin activity have been followed through time. Two peaks are reported: the first one between 4 and 12 h, concomitant with a high proteolytic activity, involves both plasmin and somatic cells while the second, between 25 and 36 h, occurring with a lower proteolytic activity involves mainly cellular proteolysis [24,26].…”

Section: F Moussaoui Et Almentioning

confidence: 99%

“…In the case of a lipopolysaccharide (LPS) infusion, plasmin and somatic cell proteases are the main origins of endogenous proteolysis [26].…”

Section: F Moussaoui Et Almentioning

confidence: 99%

Proliferation and immunoglobulin production of hybridoma cells cultured on mastitic whey

Moussaoui¹,

Roux²,

Laurent³

2003

Lait

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-Lipopolysaccharide experimental mastitis was performed in order to obtain the same milk composition change involved in a clinical mastitis without, however, any bacterial proteolytic participation. Whey from milk with a high somatic cell count (SCC) had a lower mitogenic effect on anti-human prolactin-hybridoma (anti-hPL) cell culture but a greater immunostimulating effect than that of the fetal calf serum regularly used in cell culture. This immunostimulating effect was more pronounced when expressed per cell, with an effect two-to three-fold higher than that of fetal calf serum (P < 0.01), which illustrated the efficiency of such a complement. Cell culture / whey / proteolysis / mastitisRésumé -Prolifération et production d'immunoglobulines d'hybridomes cultivés sur lactosérum de mammite. Une mammite expérimentale à lipopolysaccharide a été menée en vue d'obtenir le même changement de composition du lait que celui observé dans les cas de mammite clinique, en éliminant toutefois toute interaction avec la protéolyse bactérienne. Le lactosérum issu d'un lait à fort dénombrement cellulaire (DC) avait un effet mitogénique plus faible sur une culture cellulaire d'hybridomes anti-prolactine humaine (anti-hPL) mais toutefois un effet immunostimulant plus important que le sérum foetal de veau. Cet effet immunostimulant a été plus marqué lorsqu'il était exprimé par cellule, avec un effet 2 à 3 fois supérieur à celui observé avec le sérum de veau foetal (P < 0,01), traduisant l'efficacité de la complémentation par un lactosérum issu d'un lait à fort DC.Culture cellulaire / lactosérum / protéolyse / mammite

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Mechanisms Involved in Milk Endogenous Proteolysis Induced by a Lipopolysaccharide Experimental Mastitis

Cited by 29 publications

References 32 publications

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Polymorphonuclear proteolytic activity and milk composition change

Proliferation and immunoglobulin production of hybridoma cells cultured on mastitic whey

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