Mechanisms for the species difference between mouse and pig oocytes in their sensitivity to glucorticoids†

Gong, Shuai; Sun, Guang-Yi; Zhang, Min; Yuan, Haiqing; Zhu, Shuai; Jiao, Guiai; Luo, Ming-Jiu; Tan, Jing-He

doi:10.1093/biolre/iox026

Cited by 21 publications

(33 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, increased level of cyclic adenosine monophosphate (cAMP) or cyclic guanosine monophosphate (cGMP) promotes the GVBD in nemertean oocytes, but instead significantly blocks the GVBD in mammals [ 6 ]. In addition, physiological concentrations of glucorticoids do not affect mouse oocyte maturation, but typically inhibit the nuclear maturation of pig oocytes [ 7 ]. The non-homologous genes that are different among species may contribute to these species-specific molecular pathways.…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization

Zhao

Meng

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background: Female infertility is a worldwide concern and the etiology of infertility has not been thoroughly demonstrated. Although the mouse is a good model system to perform functional studies, the differences between mouse and human also need to be considered. The objective of this study is to elucidate the different molecular mechanisms underlying oocyte maturation and fertilization between human and mouse. Results: A comparative transcriptome analysis was performed to identify the differentially expressed genes and associated biological processes between human and mouse oocytes. In total, 8513 common genes, as well as 15, 165 and 6126 uniquely expressed genes were detected in human and mouse MII oocytes, respectively. Additionally, the ratios of non-homologous genes in human and mouse MII oocytes were 37 and 8%, respectively. Functional categorization analysis of the human MII non-homologous genes revealed that cAMP-mediated signaling, sister chromatid cohesin, and cell recognition were the major enriched biological processes. Interestingly, we couldn't detect any GO categories in mouse non-homologous genes. Conclusions: This study demonstrates that human and mouse oocytes exhibit significant differences in gene expression profiles during oocyte maturation, which probably deciphers the differential molecular responses to oocyte maturation and fertilization. The significant differences between human and mouse oocytes limit the generalizations from mouse to human oocyte maturation. Knowledge about the limitations of animal models is crucial when exploring a complex process such as human oocyte maturation and fertilization.

show abstract

Section: Introductionmentioning

confidence: 99%

RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization

Zhao

Meng

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Furthermore, in equine species, cortisol does not affect oocyte maturation in vitro [48]. The direct effect of GCs in mammalian species appears to vary greatly, implying that GC-sensitivity and regulation might be species-specific, and in part driven by diverse ratios of HSD11B2/1 [41].…”

Section: Discussionmentioning

confidence: 89%

“…Meanwhile, the gene HSD11B1, responsible for cortisone conversion into active cortisol [39,40], was downregulated in all reproductive tract segments from the cervix to the isthmus. Interestingly, in pigs, high 11β-HSD1-mediated cortisol production activity has been related to the inhibition of porcine oocyte maturation [41][42][43] in contrast to species such as the cow, where it appears to be promoted after ovulation [13,44,45] and is also beneficial for oocyte maturation and fertilization, at least in vitro [44,46,47]. Furthermore, in equine species, cortisol does not affect oocyte maturation in vitro [48].…”

Section: Discussionmentioning

confidence: 99%

Natural Mating Differentially Triggers Expression of Glucocorticoid Receptor (NR3C1)-Related Genes in the Preovulatory Porcine Female Reproductive Tract

Ruiz-Conca

Gardela

Martínez

et al. 2020

IJMS

View full text Add to dashboard Cite

Mating initiates dynamic modifications of gene transcription in the female reproductive tract, preparing the female for fertilization and pregnancy. Glucocorticoid signaling is essential for the homeostasis of mammalian physiological functions. This complex glucocorticoid regulation is mediated through the glucocorticoid receptor, also known as nuclear receptor subfamily 3 group C member 1 (NR3C1/GR) and related genes, like 11β-hydroxysteroid dehydrogenases (HSD11Bs) and the FK506-binding immunophilins, FKBP5 and FKBP4. This study tested the transcriptome changes in NR3C1/GR regulation in response to natural mating and/or cervical deposition of the sperm-peak ejaculate fraction collected using the gloved-hand method (semen or only its seminal plasma), in the preovulatory pig reproductive tract (cervix to infundibulum, 24 h after mating/insemination/infusion treatments). Porcine cDNA microarrays revealed 22 NR3C1-related transcripts, and changes in gene expression were triggered by all treatments, with natural mating showing the largest differences, including NR3C1, FKBP5, FKBP4, hydroxysteroid 11-beta dehydrogenase 1 and 2 (HSD11B1, HSD11B2), and the signal transducer and activator of transcription 5A (STAT5A). Our data suggest that natural mating induces expression changes that might promote a reduction of the cortisol action in the oviductal sperm reservoir. Together with the STAT-mediated downregulation of cytokine immune actions, this reduction may prevent harmful effects by promoting tolerance towards the spermatozoa stored in the oviduct and perhaps elicit spermatozoa activation and detachment after ovulation.

show abstract

“…The HSD11B2-led inactivation of cortisol is likely to play an important role in protecting oocytes from the adverse effects of glucocorticoids during the maturation process. Pharmaceutical levels of glucocorticoids suppress oocyte maturation in mice (30, 31, 32), sheep (33) and pigs (32, 34). However, in the presence of 18β-glycyrrhetinic acid (GA), a specific inhibitor of HSD11B2, lower levels of cortisol suppressed the oocyte maturation in mice and pigs (32).…”

Section: Discussionmentioning

confidence: 99%

Activation of HSD11B1 in the bovine cumulus-oocyte complex during IVM and IVF

Tetsuka

Tanakadate

2019

Endocrine Connections

View full text Add to dashboard Cite

The bovine cumulus-oocyte complex (COC) is capable of converting cortisone, an inert glucocorticoid to active cortisol. This mechanism is mediated by 11β-hydroxysteroid oxidoreductase type 1 (HSD11B1), whose expression dramatically increases in the mature COC. In this study, we investigate the time course expression of HSD11B1 and the enzyme activity in the bovine COC undergoing maturation and fertilization in relation to key events taking place in the COC. Bovine COCs were subjected to in vitro maturation (IVM) and fertilization (IVF). The activities of HSD11B1 and HSD11B2, which mediates the opposite reaction, were measured using a radiometric conversion assay. In parallel studies, cumulus expansion, P4 production and the expression of genes associated with ovulation were measured. The reductive activity of HSD11B1 increased in the latter half of IVM and remained high during IVF, whereas the oxidative activity of HSD11B2 remained unchanged over both periods. Consequently, the net glucocorticoid metabolism in the bovine COC shifted from inactivation to activation around the time of ovulation and fertilization. The increase in HSD11B1 expression lagged behind that of P4 increase and cumulus expansion but ahead of the expressions of genes responsible for PGE2 synthesis. The reductive activity of HSD11B1 was well correlated with the cumulus expansion rate. This outcome indicates that the ability of the cumulus to activate glucocorticoids is related to its ability to synthesize hyaluronan. These results also indicate that the activation of HSD11B1 is an integral part of the sequential events taking place at the ovulation and fertilization in the bovine COC.

show abstract

Mechanisms for the species difference between mouse and pig oocytes in their sensitivity to glucorticoids†

Cited by 21 publications

References 25 publications

RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization

RNA-Seq transcriptome reveals different molecular responses during human and mouse oocyte maturation and fertilization

Natural Mating Differentially Triggers Expression of Glucocorticoid Receptor (NR3C1)-Related Genes in the Preovulatory Porcine Female Reproductive Tract

Activation of HSD11B1 in the bovine cumulus-oocyte complex during IVM and IVF

Contact Info

Product

Resources

About