Mechanisms for Colonic Sodium Transport during Ontogeny: Loss of an Amiloride-Sensitive Sodium Pathway

Finkel, Yigael; Eklöf, Ann-Christine; Aperia, Anita

doi:10.1203/00006450-198807000-00012

Cited by 13 publications

(9 citation statements)

References 15 publications

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“…Several studies have provided strong evidence that aldosterone increases Na + absorption in the adult rat distal colon by inhibiting electroneutral Na + /H + exchange and inducing electrogenic Na + absorption via Na + channels (Binder et al 1991). Even if Na + absorption is increased and electrogenic amiloride-sensitive Na + transport pathway dominates in the immature rat distal colon (Pácha et al 1987;Finkel et al 1988), we have not observed any parallel changes in aSCC, plasma level of aldosterone or apical membrane area. This means that the incorporation of Na + channels into the apical membrane occurs without any increase in the membrane domain and that the regulation of the Na + pathway in the immature rat distal colon by aldosterone is localized predominantly in the apical membrane without any significant correlation between its ultrastructure and Na + transport.…”

Section: Groupcontrasting

confidence: 73%

“…Since the absorptive capacity and basolateral or apical cell membrane surface areas are related in the large intestine (Kashgarian et al 1980;Elbrond et al 1991), we posed the question of whether such a relationship also exists during the developmental period of rapidly changing colonic absorption (Finkel et al 1988;. Our principal finding is that the basolateral but not apical membrane area increases markedly during weaning and that the morphological changes correlate well with the observed functional changes.…”

Section: Discussionmentioning

confidence: 48%

“…In particular, Na + transport and Na, KATPase activity exhibit considerable ontogenetic changes. In the rat colon, Na, K-ATPase activity reveals an agedependent increase (Pácha et al 1991), although Na + absorption is very high during the suckling period and significantly decreases later (Finkel et al 1988). The very high Na + absorption is predominantly a consequence of electrogenic amiloride-sensitive Na + transport which increases during the suckling period, reaches a peak during weaning and entirely disappears later (Pácha et al 1987.…”

Section: Introductionmentioning

confidence: 96%

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Correlation of function and structure in developing rat distal colon

Vagnerová

Kubínová

Pácha

1997

Cell and Tissue Research

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The distal colon of suckling (14-day-old) and weanling (27-day-old) rats absorbs Na+ via channel-mediated, electrogenic amiloride-sensitive Na+ transport which disappears after weaning. This transport pathway is induced by aldosterone in superficial cells of colonic epithelium. The purpose of the present study was to correlate the changes in distal colon function with changes in the apical and basolateral cell membrane surface areas of superficial enterocytes. The basolateral but not apical membrane surface density (i.e. surface areas of the basolateral and apical membranes of superficial enterocytes per unit volume of superficial enterocytes) was found to increase between postnatal day 14 and 27 (i.e. during the weaning period). The plasma concentrations of aldosterone were very high during this period and electrogenic amiloride-sensitive Na+ transport and Na, K-ATPase activity were increased significantly. High dietary salt intake during the weaning period prevented the developmental increase of basolateral membrane surface density, inhibited electrogenic amiloride-sensitive Na+ transport and significantly depressed plasma aldosterone levels and Na, K-ATPase activity. Apical cell membrane surface density did not change significantly after a sodium load. Thus, high plasma concentrations of aldosterone and/or high colonic Na+ transport during weaning exert an important and selective effect on the basolateral membrane and are responsible for the developmental changes of this cell membrane surface during weaning. Concomitant changes in the morphology of superficial colonic enterocytes and in colonic Na+ transport suggest that the structure of the immature epithelium is related to its function.

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Section: Groupcontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 48%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Correlation of function and structure in developing rat distal colon

Vagnerová

Kubínová

Pácha

1997

Cell and Tissue Research

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“…High Na + absorption in the neonatal period and the reduced Na + absorption in postnatal life are observed in the colon of rat, rabbit, and human (86, 132, 216), and also in the rat small intestine (227). The Na + absorptive pathway in the colon is mainly mediated by amiloride-sensitive Na + channels (216, 228, 229).…”

Section: Small Intestinal Na+/h+ Exchangementioning

confidence: 99%

SLC9 Gene Family: Function, Expression, and Regulation

Kiela

2018

Comprehensive Physiology

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The Slc9 family of Na /H exchangers (NHEs) plays a critical role in electroneutral exchange of Na and H in the mammalian intestine as well as other absorptive and secretory epithelia of digestive organs. These transport proteins contribute to the transepithelial Na and water absorption, intracellular pH and cellular volume regulation as well as the electrolyte, acid-base, and fluid volume homeostasis at the systemic level. They also influence the function of other membrane transport mechanisms, affect cellular proliferation and apoptosis as well as cell migration, adherence to the extracellular matrix, and tissue repair. Additionally, they modulate the extracellular milieu to facilitate other nutrient absorption and to regulate the intestinal microbial microenvironment. Na /H exchange is inhibited in selected gastrointestinal diseases, either by intrinsic factors (e.g., bile acids, inflammatory mediators) or infectious agents and associated bacterial toxins. Disrupted NHE activity may contribute not only to local and systemic electrolyte imbalance but also to the disease severity via multiple mechanisms. In this review, we describe the cation proton antiporter superfamily of Na /H exchangers with a particular emphasis on the eight SLC9A isoforms found in the digestive tract, followed by a more integrative description in their roles in each of the digestive organs. We discuss regulatory mechanisms that determine the function of Na /H exchangers as pertinent to the digestive tract, their regulation in pathological states of the digestive organs, and reciprocally, the contribution of dysregulated Na /H exchange to the disease pathogenesis and progression. © 2018 American Physiological Society. Compr Physiol 8:555-583, 2018.

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“…We believe that the change in effect of amiloride during postnatal development in the sheep is more likely to reflect the progressive acquisition of an alternative pathway for Na+ that is insensitive to the presence of amiloride. It is of interest in this respect that the colon of the rat displays developmental changes in an amiloridesensitive sodium pathway (Finkel, Eklof & Aperia, 1988) that are similar to those in the sheep lung. At 20 days of postnatal life amiloride completely blocked net sodium absorption from the lumen of the rat colon yet by 40 days of life it had no effect at all although sodium absorption was still present (albeit at a lower rate).…”

Section: Mechanisms Of Liquid Absorptionmentioning

confidence: 99%

Liquid flow across the epithelium of the artificially perfused lung of fetal and postnatal sheep.

Ramsden¹,

Markiewicz²,

Walters³

et al. 1992

The Journal of Physiology

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SUMMARY1. The lungs of five fetal (133-140 days gestation) and thirty-four postnatal (2-240 days) sheep were artificially perfused in situ with warmed and oxygenated sheep blood. In postnatal animals the airspace of the lung was filled with liquid similar in composition to fetal lung liquid. In fetal and postnatal animals luminal liquid volume was measured by the impermeant tracer technique.2. Under resting conditions the pulmonary epithelium of fetal animals secreted liquid at a mean (±s.E.M.) rate of 2-0 (±0 4) ml (kg body weight)-' h-', those of postnantal animals absorbed liquid at -1-8 (±0 2) ml (kg body weight)-' h-1.3. Addition of 2,4-dinitrophenol to achieve a concentration of 1P5 x 10-3 M in the perfusing blood in postnatal animals caused complete cessation of liquid absorption. 4. Light and electron microscopic examination of the lung after periods of up to 6 h of artificial perfusion showed no evidence of epithelial damage. From 3 h onwards, liquid accumulation was evident in the perivascular spaces.5. Addition of adrenaline to the perfusate in fetal animals caused absorption of liquid to occur at a mean rate of -29 (± 1P3) ml (kg body weight)-' h-1. In postnatal animals adrenaline caused the rate of liquid absorption to increase from a mean rate of -14 (± 0 2) to -2-2 (± 0 3) ml (kg body weight)-' h-1.6. In the fetus addition of amiloride (0-8 x 10-4 M) to the luminal fluid blocked adrenaline-induced liquid absorption and caused secretion to occur at 1-3 (±0 3) ml (kg body weight)-' h-1.7. In postnatal animals the response to amiloride was age dependent. In newborn lambs (2-14 days) amiloride blocked liquid absorption and caused secretion of liquid to occur in seven out of eight animals at a mean rate of 09 (±03) ml (kg body weight)-' h-' (n = 8). In older animals (15-240 days) the characteristic response to amiloride was slowing of the rate of liquid absorption (mean rate of absorption, -0-2 (±0 09) ml (kg body weight)-' h-1, n = 18) with liquid secretion being seen in only three of eighteen animals.8. We conclude firstly that the mechanisms that mediate secretion of liquid by the fetal pulmonary epithelium appear to be retained in the lung for at least the first 14 days of postnatal life and secondly that the mechanisms which underlie absorption MS 9203 C. A. RAMSDEN AND OTHERS of liquid change during postnatal development with the progressive acquisition of an absorptive process that cannot be blocked by amiloride.

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Mechanisms for Colonic Sodium Transport during Ontogeny: Loss of an Amiloride-Sensitive Sodium Pathway

Cited by 13 publications

References 15 publications

Correlation of function and structure in developing rat distal colon

Correlation of function and structure in developing rat distal colon

SLC9 Gene Family: Function, Expression, and Regulation

Liquid flow across the epithelium of the artificially perfused lung of fetal and postnatal sheep.

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