Mechanisms exploration of herbal pair of HuangQi-DanShen on cerebral ischemia based on metabonomics and network pharmacology

Xue, Qianqian; Li, Aiping; Li, Ke; Qin, Xuemei

doi:10.1016/j.jep.2020.112688

Cited by 31 publications

(11 citation statements)

References 42 publications

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“…Vascular endothelial growth factor (VEGF), which is closely related to angiogenesis, is considered to be a key factor in endometrial repair ( Sugino et al, 2002 ; Maybin et al, 2011 ). Studies have shown that leonurine, the main active component of Herba Leonuri, can promote the angiogenesis of endothelial cells by activating the mTOR/ERK pathway and enhance angiogenesis both in vivo and in vitro ( Wang et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Chemical Fingerprint Analysis and Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics Study of the Protective Effect of Buxue Yimu Granule in Medical-Induced Incomplete Abortion Rats

Zhang

Chen

et al. 2020

Front. Pharmacol.

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Medical abortion is a common method to terminate an early pregnancy and often causes serious complications such as abnormal uterine bleeding and endometritis. Buxue Yimu granule (BYG) is a well-known traditional Chinese medicine prescription composed of five kinds of drugs and is widely used in gynecology and obstetrics. The aim of the present study was to establish the quality standard of BYG and investigate its protective effect on incomplete abortion. The chemical fingerprint of BYG was established by high performance liquid chromatography (HPLC). The major compounds of BYG were determined by ultra-performance liquid chromatography with triple quadrupole mass spectrometry. An incomplete abortion rat model was induced by intragastric administration of mifepristone (8.3 mg·kg−1) combined with misoprostol (100.0 μg·kg−1) during early pregnancy. The serum levels of human chorionic gonadotrophin (HCG), estradiol (E2), and progesterone (PG) were determined. The serum endogenous metabolites were analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Multivariate analysis, including partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA), was employed to analyze the metabolic profiles, and MetaboAnalyst was used to investigate the metabolic pathways. Furthermore, hematoxylin-eosin staining (HE) was used to evaluate the histopathological changes in uterine tissue. The expression levels of VEGFA and NF-κB were detected by immunohistochemistry. The results indicated that HPLC fingerprint analysis can be successfully used to assess the quality of BYG. The medical-induced incomplete abortion rats were clearly separated from control rats, and the biochemical changes were gradually restored to normal after administration of BYG. Moreover, 19 potential biomarkers, including N-lactoylleucine, 2-piperidinone, isobutyryl-l-carnitine, eicosapentaenoylcholine, LysoPC(14:0), LysoPC(20:5), physagulin C, LysoPC(18:3), leukotriene D5, deoxycholic acid 3-glucuronide, glycine, pregnanediol 3-O-glucuronide, LysoPC(18:2), LysoPC(17:0/0:0), N-acetyl-leukotriene E4, LysoPC(18:0), platelet-activating factor, LysoPA(24:1), and LysoPC(18:1), which were mainly related to the amino acids metabolism, lipids metabolism, and bile acid biosynthesis, were identified. Consequently, BYG exerts a potential protective role in the intervention of incomplete abortion by anti-inflammatory, promote endometrial repair, and regulate the metabolic disorders.

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Section: Discussionmentioning

confidence: 99%

Chemical Fingerprint Analysis and Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics Study of the Protective Effect of Buxue Yimu Granule in Medical-Induced Incomplete Abortion Rats

Zhang

Chen

et al. 2020

Front. Pharmacol.

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“…All chemical ingredients of each herb in GGQLD were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) (https://tcmspw.com/tcmsp.php) [17].…”

Section: Construction Of Ggqld Chemical Ingredients Databasementioning

confidence: 99%

Uncovering the Mechanism of Ge-Gen-Qin-Lian Decoction for Treating Ulcerative Colitis Based on Network Pharmacology and Molecular Docking Verification

Zhang

et al. 2020

Preprint

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Background and objective: Ge-Gen-Qin-Lian Decoction (GGQLD), a traditional Chinese medicine (TCM) formula, has been widely used for ulcerative colitis (UC) in China while the pharmacological mechanisms still remain unclear. The present research was designed to clarify the underlying mechanism of GGQD against UC. Methods: In this research, a GGQLD-compound-target-UC (G-U) network was constructed based on public databases to clarify the relationship between active compounds in GGQLD and potential targets. GO and KEGG pathway enrichment analyses were performed to investigate biological functions associated with potential targets. A protein-protein interaction network was constructed to screen and evaluate hub genes and key active ingredients, another GO and KEGG pathway analyses were subsequently performed on hub genes. Molecular docking was used to verify the activities of binding between hub targets and ingredients. Results: Finally, 83 potential therapeutic targets and 118 correspond active ingredients were obtained by network pharmacology. GO and KEGG enrichment analysis revealed that GGQLD had an effect of anti-inflammation, antioxidation, and immunomodulatory. The effect of GGQLD on UC might be achieved by regulating the balance of cytokines (eg., IL6, TNF, IL1β, CXCL8, CCL2, IL10, IL4, IL2) in immune system and inflammation-related pathways, such as IL-17 pathway and Th17 cell differentiation pathway. Besides, molecular docking results demonstrated that the main active ingredients, quercetin, exhibited good affinity to hub targets. Conclusion: This research fully reflects the characteristics of multi-component and multi-target for GGQLD in the treatment of UC. Furthermore, the present study provided new insight into the mechanisms of GGQLD against UC.

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“…Compared with TCM formulas containing more herbs, it is simpler and more bene-cial to elucidate the complex pharmacological mechanism of a herbal pair. 10 Eucommia ulmoides (EU, Chinese name: Duzhong) is the bark of Eucommia ulmoides Oliv., a typical Chinese herb for tonifying the kidney, while Dipsaci Radix (DR, Chinese name: Xuduan) is the dry root of Dipsacus asper Wall., another natural herb for strengthening the bone. 11,12 Previous studies have shown that EU and DR are currently the most widely used TCM for the treatment of OP.…”

Section: Introductionmentioning

confidence: 99%

Exploring the potential therapeutic effect of Eucommia ulmoides–Dipsaci Radix herbal pair on osteoporosis based on network pharmacology and molecular docking technology

et al. 2022

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Mechanisms exploration of herbal pair of HuangQi-DanShen on cerebral ischemia based on metabonomics and network pharmacology

Cited by 31 publications

References 42 publications

Chemical Fingerprint Analysis and Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics Study of the Protective Effect of Buxue Yimu Granule in Medical-Induced Incomplete Abortion Rats

Chemical Fingerprint Analysis and Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics Study of the Protective Effect of Buxue Yimu Granule in Medical-Induced Incomplete Abortion Rats

Uncovering the Mechanism of Ge-Gen-Qin-Lian Decoction for Treating Ulcerative Colitis Based on Network Pharmacology and Molecular Docking Verification

Exploring the potential therapeutic effect of Eucommia ulmoides–Dipsaci Radix herbal pair on osteoporosis based on network pharmacology and molecular docking technology

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