Mechanisms associated with phagocytosis of Arcobacter butzleri by Acanthamoeba castellanii

Medina, Gustavo; Flores-Martin, Sandra N.; Fonseca, Belchiolina Beatriz; Otth, Carola; Fernández, Heriberto

doi:10.1007/s00436-014-3842-8

Cited by 31 publications

(21 citation statements)

References 37 publications

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“…However, some authors have reported the implication of mannose-binding proteins (Alsam et al 2005) and/or membrane-associated receptors of glucose or galactose in the attachment of bacteria or yeasts to A. castellanii (Declerck et al 2007;Medina et al 2014). Under the conditions in the current study, the addition of mannose or mannan had an effect on conidial uptake by the macrophagic cells only, indicating the active role of these saccharide receptors in the attachment process.…”

Section: Discussioncontrasting

confidence: 43%

“…According to the methods of Medina et al (2014), monolayers of amoebae trophozoites or THP-1 cells were pretreated with 100 mM glucose, galactose, mannose, or mannane dissolved in PAS for amoebae (or PAS-RPMI 1640 v/v for THP-1 cells) for 90 min at 27°C for amoebae or at 37°C under 5 % CO 2 for macrophages. After washing with PAS or PAS-RPMI 1640, A. fumigatus conidia at a MOI = 5 were added to the different monolayers and incubated at 27°C (FLA) or at 37°C under 5 % CO 2 (macrophages) during 1 h. Wells were washed three times with the appropriate buffer (to remove unattached conidia), and the contents of the wells were plated on Sabouraud agar plates to number fungal CFU.…”

Section: Attachment Inhibitory Assaysmentioning

confidence: 99%

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Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells

et al. 2016

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Free living amoebae (FLA) are protists ubiquitously present in the environment. Aspergillus fumigatus is a mould responsible for severe deep-seated infections, and that can be recovered in the same habitats as the FLA. By conducting coculture experiments and fungal incubation with amoebal supernatants, we report herein that Vermamoeba vermiformis, a FLA present in hospital water systems, promotes filamentation and growth of A. fumigatus. This finding is of particular importance to institutions whose water systems might harbor FLA and could potentially be used by immunocompromised patients. Also, the relationships between V. vermiformis and A. fumigatus were compared to those between this fungus and two other phagocytic cells: Acanthamoeba castellanii, another FLA, and macrophage-like THP-1 cells. After 4 h of coincubation, the percentages of the three phagocytic cell types with adhered conidia were similar, even though the types of receptors between FLA and macrophagic cell seemed different. However, the percentage of THP-1 with internalized conidia was considerably lower (40 %) in comparison with the two other cell types (100 %). Thus, this study revealed that interactions between A. fumigatus and these three phagocytic cell types show similarities, even though it is premature to extrapolate these results to interpret relationships between A. fumigatus and macrophages.

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Section: Discussioncontrasting

confidence: 43%

Section: Attachment Inhibitory Assaysmentioning

confidence: 99%

Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells

et al. 2016

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“…jejuni can survive and even multiply within free-living amoebae, establishing endosymbiotic relationships (OLOFSSON et al, 2013). This kind of symbiosis, between bacteria and protozoa, particularly Acanthamoeba castellanii, was also reported to Arcobacter butzleri, other species of family Campylobacteraceae (FERNÁNDEZ et al, 2012;MEDINA et al, 2014).…”

Section: Development Epidemiologymentioning

confidence: 96%

Campylobacteriosis: an emerging zoonosis, underdiagnosed and underreported by public health agencies in Brazil

et al. 2015

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ABSTRACT:The infection by Campylobacter spp. constitutes a zoonosis of worldwide distribution, with serious repercussions for public health and a significant socio-economic impact. The Campylobacter species that most often are implicated in outbreaks of gastrointestinal disease are C. jejuni and C. coli. Both are recognized as the most important and with greater pathogenic potential from the viewpoint of food safety. This review aims to clarify and discuss, based on literature, the epidemiology of Campylobacter spp. and the main challenges in their control, to assist in better understanding of the risk posed to human health. Due to the difficulty of isolating and cultivating of Campylobacter spp., the high cost and not mandatory, laboratory diagnosis by traditional cultivation techniques is not widely used in Brazil, resulting in lack of accurate data on the occurrence of the disease in the country. This factor has been identified as responsible for underdiagnosing and underreporting of the disease, which means that data on contamination of animal products or outbreaks are not statements of actual national situation. The epidemiology of Campylobacter spp. is considered complex presenting multiple determinants in the spread of this microorganism. Chickens are important reservoirs of Campylobacter sp. and the main target of control measures, however, other vehicles of transmission, such as raw cow milk, drinking water and other foods of animal origin must also be considered in the set of measures to be taken to control disease. The infection by Campylobacter spp. cause gastroenteritis in humans and occurred by oral contact with infected animals. In fact, are pets or production and food consumption, the most important pathways. The clinical manifestations of the disease can be from mild to severe symptoms, including watery diarrhea, sometimes with blood, abdominal pain, fever, headache and nausea. Sometimes the infection can get complications after the diarrhea stops and starts a progressive paralysis that can result in respiratory failure and severe neurological dysfunction and may even lead to death. The occurrence of campylobacteriosi's outbreaks should be mandatory to the authorities of surveillance, to provide an investigation of common sources and transmission control through preventive measures , thereby minimizing the potential risks to human health.

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“…The genus Arcobacter is evolving rapidly from the day it has been defined. Recently, a symbiotic relationship of Arcobacter with Acanthamoeba castellanii has been identified showing its potential to survive and escape phagocytosis by amoeba (Medina et al 2014). Evolution of this emerging food-borne pathogen needs to be elucidated in a better way so as to find out the epidemiology and virulence factors of this pathogen.…”

Section: Evolution and Emergence Of Arcobactersmentioning

confidence: 99%

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

127

107

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Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries. Arcobacters are implicated as causative agents of diarrhea, mastitis and abortion in animals, while causing bacteremia, endocarditis, peritonitis, gastroenteritis and diarrhea in humans. Three species including A. butzleri, A. cryaerophilus and A. skirrowii are predominantly associated with clinical conditions. Arcobacters are primarily transmitted through contaminated food and water sources. Identification of Arcobacter by biochemical tests is difficult and isolation remains the gold standard method. Current diagnostic advances have provided various molecular methods for efficient detection and differentiation of the Arcobacters at genus and species level. To overcome the emerging antibiotic resistance problem there is an essential need to explore the potential of novel and alternative therapies. Strengthening of the diagnostic aspects is also suggested as in most cases Arcobacters goes unnoticed and hence the exact epidemiological status remains uncertain. This review updates the current knowledge and many aspects of this important food-borne pathogen, namely etiology, evolution and emergence, genetic diversity, epidemiology, the disease in animals and humans, public health concerns, and advances in its diagnosis, prevention and control.

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Mechanisms associated with phagocytosis of Arcobacter butzleri by Acanthamoeba castellanii

Cited by 31 publications

References 37 publications

Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells

Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells

Campylobacteriosis: an emerging zoonosis, underdiagnosed and underreported by public health agencies in Brazil

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

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