Mechanisms associated with abnormal E-cadherin immunoreactivity in human bladder tumors

Bringuier, Pierre Paul; Giroldi, Laurence A.; Umbas, H. Rainy; Shimazui, Toru; Schalken, Jack A.

doi:10.1002/(sici)1097-0215(19991126)83:5<591::aid-ijc3>3.0.co;2-6

Cited by 25 publications

(10 citation statements)

References 24 publications

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“…Recently, Bringuier et al showed that abnormal E-cadherin immunoreactivity in bladder tumors is associated with mRNA downregulation or post-transcriptional downregulation of E-cadherin. In the same series of bladder tumors, no structural alterations of the E-cadherin gene were detected [5]. These findings corroborate with our observations on the transient reduction of E-cadherin expression in invasive bladder tumors.…”

Section: Discussionsupporting

confidence: 91%

Influence of the microenvironment on invasiveness of human bladder carcinoma cell lines

et al. 2001

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To investigate the importance of the microenvironment in bladder cancer invasion, a panel of six bladder carcinoma cell lines (SD, RT112, JON, 1207, T24, and J82) was tested in both in vitro and in vivo invasion assays. Furthermore, invasiveness was correlated with the expression of components of the E-cadherin-catenin complex. The E-cadherin-negative cell lines, T24 and J82, displayed a high in vitro invasive capacity, whereas the E-cadherin-positive cell lines, SD and JON, completely lacked in vitro invasive capacity. In contrast, in vivo invasion was noted for all cell lines, with the exception of cell line JON. Most notably, SD formed highly invasive tumors in vivo. The in vivo invasiveness of the E-cadherin-positive bladder carcinoma cell lines was associated with a heterogeneous expression of the E-cadherin-catenin complex. The discrepancy between in vitro and in vivo invasive behavior implies that, in vivo, the microenvironment plays an important role in the establishment of the invasive phenotype. In addition, it was found that orthotopic xenografting of 1207 and T24 bladder carcinoma cells resulted in sitespecific tumor take and an enhanced tumor outgrowth and invasiveness, respectively, compared with heterotopic (i.e., subcutaneous) inoculation. We conclude that the site-specific growth and invasion of the bladder carcinoma cell lines in vivo and the observed assay specific invasion (in vitro vs in vivo) points to an effect of the local (bladder) microenvironment on tumor cell behavior.

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Section: Discussionsupporting

confidence: 91%

Influence of the microenvironment on invasiveness of human bladder carcinoma cell lines

et al. 2001

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“…This loss of function is thought to be due to a combination of gene mutation, promotor methylation and loss of heterozygosity 23,24 . The mechanism of abnormal E‐cadherin expression in unselected carcinoma of the bladder is less well established 25,26 . Further study is needed to explain the loss of E‐cadherin expression in the discohesive variant of urothelial carcinoma described in this study.…”

Section: Discussionmentioning

confidence: 84%

Transitional cell carcinoma of the bladder mimicking lobular carcinoma of the breast: a discohesive variant of urothelial carcinoma

et al. 2005

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“…27 In the same way, highly metastatic unstable E-cadherin expression that varied readily with cell culture conditions has been reported. 28 Thus, heterogeneous E-cadherin expression might be assigned not only to tumor heterogeneity but also to unstable expression in vivo. As in most reported studies, 11,13,17,25,29 we did not find any association between reduced E-cadherin expression and tumor grade, whereas others have shown E-cadherin staining to be associated with tumor grade.…”

Section: Discussionmentioning

confidence: 99%

Expression of E-Cadherin and α-,β-,γ-Catenins in Patients With Bladder Cancer

Clairotte¹,

Lascombe²,

Fauconnet³

et al. 2006

Am J Clin Pathol

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Loss of intercellular adhesion facilitates tumor invasion. To clarify the relation between altered expression of cell adhesion molecules and progression of T1 superficial bladder tumors, 101 cases (71 T1 tumors, 30 T2/T3 tumors) were examined immunohistochemically for E-cadherin and alpha-, beta-, and gamma-catenins. A highly significant correlation was observed between the decreased expression of all molecules and increased TNM stage (P < .001). Univariate analysis, performed in cases of T1 tumors, revealed association of abnormal E-cadherin with beta-catenin diminution. Survival curves were established with the Kaplan-Meier method and analyzed according to clinical and histopathologic parameters using the log-rank test. Cox multivariate analysis revealed only gamma-catenin as an independent predictor of progression-free survival in patients with stage T1 bladder urothelial tumors. The characterization of T1 tumors that will progress could lead to the identification of patients who might benefit from surgery to avoid vesical muscle invasion and, consequently, metastasis.

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Mechanisms associated with abnormal E-cadherin immunoreactivity in human bladder tumors

Cited by 25 publications

References 24 publications

Influence of the microenvironment on invasiveness of human bladder carcinoma cell lines

Influence of the microenvironment on invasiveness of human bladder carcinoma cell lines

Transitional cell carcinoma of the bladder mimicking lobular carcinoma of the breast: a discohesive variant of urothelial carcinoma

Expression of E-Cadherin and α-,β-,γ-Catenins in Patients With Bladder Cancer

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