The hospital is a facility where numerous diseases are treated. However, the hospital is also a site where numerous diseases, such as infection, can
be contracted. Large quantities of liquid waste are produced by hospitals in Indonesia. This liquid waste is contagious, contains pathogenic bacteria
and toxic compounds that are hazardous to human health and the environment. Antibiotics were detected as a component of hospital wastewater in
previous research, and the majority of bacteria isolated from this wastewater were shown to be resistant to routinely used antibiotics. By isolating
and identifying bacteria, this study intends to detect antibiotic contamination in the liquid waste disposal system at Ratatotok General Hospital,
Southeast Minahasa. The methodology employed is quantitative descriptive research. The sample consists of liquid waste from the site of
discharge. Bacterial isolation and identication according to Bergey's Manual of Determinative Bacteriology. For the sensitivity test employing the
Kirby Bauer method and antibiotic, Amoxicillin and Gentamicin is employed, and the results are interpreted according to CLSI guidelines. Based
on the results of the study, the bacteria Streptococcus spp, Escherichia spp and Staphylococcus spp were identied in the wastewater at Ratatotok
General Hospital, Southeast Minahasa. The bacteria identied showed an interpretation of being resistant to the antibiotic Amoxicillin and some
bacteria is still sensitive for Gentamicin.