Mechanisms and Genetics of Resistance to Sodium Lauryl Sulfate in Strains of
            <i>Shigella</i>
            and
            <i>Escherichia coli</i>

Corwin, Laurence M.; Rothman, Sara W.; Kim, Rosalind; Talevi, Leonard A.

doi:10.1128/iai.4.3.287-294.1971

Cited by 20 publications

(14 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Clinical observations strongly suggest that only antibiotic-sensitive gonococci are capable of causing systemic infection in man (32). Penicillin-resistant envelope mutants of salmonella are more sensitive to host antibody (22), and detergent, lysozyme, and EDTA-resistant mutants of shigella are less invasive (7). One or more of the putative envelope loci (ery, env) which we have described in the gonococcus may be associated with decreased virulence, although this and several other questions require further study.…”

Section: Discussionmentioning

confidence: 99%

Altered crystal violet permeability and lytic behavior in antibiotic-resistant and -sensitive mutants of Neisseria gonorrhoeae

Guymon¹,

Sparling²

1975

J Bacteriol

View full text Add to dashboard Cite

Wild-type, antibiotic-resistant and hypersensitive isogenic strains of Neisseria gonorrhoeae were studied for uptake of crystal violet, rates of autolysis, and response to lysozyme. Total uptake of crystal violet was similar in all strains at 0 C but varied significantly at 37 C. Mutation at the nonspecific resistance locus ery resulted in relative impermeability to crystal violet at 37 C, as compared to wild type. The penetration barrier to crystal violet at 37 C was overcome by addition of 5 mM ethylenediaminetetraacetic acid. Mutation at ery also resulted in reduced rates of autolysis and reduced sensitivity to high concentrations of lysozyme under conditions of divalent cation (Mg2+) depletion. In contrast, mutation at the nonspecific drug hypersensitivity locus env resulted in increased uptake of crystal violet at 37 C, due to increased binding of dye to crude envelope as well as increased penetration into cytoplasm. The env mutants were also more rapidly autolytic and more sensitive to lysozyme than wild type in the absence of Mg2+. These results suggest that the cell envelopes of ery mutants are more stable and less permeable and those of env mutants are less stable and more permeable than wild-type strains.

show abstract

Section: Discussionmentioning

confidence: 99%

Altered crystal violet permeability and lytic behavior in antibiotic-resistant and -sensitive mutants of Neisseria gonorrhoeae

Guymon¹,

Sparling²

1975

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Thus, hydrolysis of phospholipids may enhance the reorgan-ization of the outer membrane. Other enterobacterial mutants resistant to Tris-EDTA-induced permeability increase are the polymyxin-resistant pmrA strains of S. typhimurium, which have an altered LPS (see below), and the less wellcharacterized strains of E. coli, studied by Voll and Leive (325), and Shigellaflexneri, studied by Corwin et al (48). All of these mutants release less LPS upon EDTA treatment than do the corresponding wild-type strains.…”

Section: Effects Of Edtamentioning

confidence: 99%

Molecular basis of bacterial outer membrane permeability.

Nikaido¹,

Vaara²

1985

Microbiological Reviews

1,345

684

View full text Add to dashboard Cite

are very toxic to gram-positive bacteria (57, 230, 242). In enteric gram-negative bacteria, which live in the intestinal tract of animals, the outer membrane has developed into a very effective barrier, giving protection to cells from the detergent action of bile salts and degradation by digestive enzymes (207). At the same time the outer membrane of enteric and some other gram-negative bacteria acts as a strong permeability barrier to many antibiotics that are effective against other bacteria (e.g., macrolides, novobiocin, rifamycins, lincomycin, clindamycin, and fusidic acid; see reference 207). Even when the diffusion of antibiotic is merely slowed down by the presence of outer membrane, the bacteria can then inactivate the small amount of penetrating antibiotic rather than try to inactivate the almost infinite amount of antibiotic present in the medium, and thus very high levels of resistance are easily established in 1

show abstract

“…MOPSwashed cells were also significantly more sensitive to cadmium. The ability of SDS to lyse cells is apparently related to the ionic strength of the suspension medium (Corwin et al, 1971).…”

Section: Effect Of Cell Washingmentioning

confidence: 99%

Inhibition of β‐galactosidase biosynthesis in Escherichia coli: Effect of alterations of the outer membrane permeability on sensitivity to environmental toxicants

Dutton

Bitton

Koopman

et al. 1990

Environ. Toxicol. Water Qual.

View full text Add to dashboard Cite

The use of bacteria as test organisms in rapid ecotoxicity assays is under investigation in several laboratories worldwide. However, little attention has been given to the question of permeability of the outer membrane of gram-negative bacteria to toxicants of environmental interest. We have investigated the effect of physical, chemical, and genetic alterations on the sensitivity ofEscherichia coli to environmental toxicants, as measured via inhibition of P-galactosidase biosynthesis. Polymyxin treatment (2 mg/L) was the most promising treatment tested, significantly increasing the sensitivity of wild-type E. coli to pentachlorophenol and sodium dodecyl sulfate. A mutant strain ofE. coli (EWlb), with an outer membrane protein alteration (tol C gene), was found to be the most sensitive to hydrophobic compounds and to detergent. EWlb, further sensitized via polymyxin treatment, appears to be a sensitive microorganism for toxicant assay.

show abstract

Mechanisms and Genetics of Resistance to Sodium Lauryl Sulfate in Strains of Shigella and Escherichia coli

Cited by 20 publications

References 6 publications

Altered crystal violet permeability and lytic behavior in antibiotic-resistant and -sensitive mutants of Neisseria gonorrhoeae

Altered crystal violet permeability and lytic behavior in antibiotic-resistant and -sensitive mutants of Neisseria gonorrhoeae

Molecular basis of bacterial outer membrane permeability.

Inhibition of β‐galactosidase biosynthesis in Escherichia coli: Effect of alterations of the outer membrane permeability on sensitivity to environmental toxicants

Contact Info

Product

Resources

About