Mechanism underlying histamine-induced intracellular Ca 2+movement in PC3 human prostate cancer cells

NPC-14686 (Fmoc-l-homophenylalanine)-induced Ca2+ increases and death in human prostate cancer cells

“…Thus it is pivotal to investigate the effect of a reagent on cellular Ca 2+ handling in order to understand its in vitro action. To further clarify the effect of NPC-14686 on Ca 2+ movement, in this study, by using fura-2 as a fluorescent Ca 2+ probe and WST-1 as a fluorescent cell growth indicator, it has been demonstrated that NPC-14686 increased [Ca 2+ ] , histamine (Lee et al, 2001) clomiphene , and organotin compounds . The IP 3 -sensitive Ca 2+ store is an important Ca 2+ store that releases Ca 2+ into the cytosol when cells are stimulated by endogenous agents such as histamine (Lee et al, 2001).…”

Nutrigenetics and Prostate Cancer: 2011 and Beyond

“…It acts by stimulating histamine receptors H1, H2 and H3 [156,157,158]. An experimental study showed that histamine increased intracellular free Ca 2+ levels ([Ca 2+ ] i ) in PC3 prostate cancer cells [155]. The Ca 2+ signal is also contributed by extracellular Ca 2+ entry and intracellular Ca 2+ release from the endoplasmic reticular intracellular Ca 2+ store [155].…”

“…Histamine is an endogenous transmitter found in most tissues, especially in the granules of mast cells [155]. It acts by stimulating histamine receptors H1, H2 and H3 [156,157,158].…”

“…An experimental study showed that histamine increased intracellular free Ca 2+ levels ([Ca 2+ ] i ) in PC3 prostate cancer cells [155]. The Ca 2+ signal is also contributed by extracellular Ca 2+ entry and intracellular Ca 2+ release from the endoplasmic reticular intracellular Ca 2+ store [155]. If SLC22A5 variants decrease the activity of the transporter, histamine uptake will also be reduced, thus increasing the cytosolic histamine levels and increasing [Ca 2+ ] i levels in prostate cancer cells.…”

Effects of MK‐886, a leukotriene synthesis inhibitor, on [Ca2+]i and apoptosis in MG63 human osteosarcoma cells

“…MG63 cells have characteristics similar to human osteoblasts and are used as a model for research on human osteoblasts [Rezzonico et al, 2002]. Many endogenous and exogenous reagents can stimulate MG63 cells by evoking a [Ca 21 ] i increase, such as 2,2 0 -dithiodipyridine [Kuo et al, 2003], carvedilol [Liu and Jan, 2004], and desipramine [Lee et al, 2001]. Exogenous ligands can also release Ca 21 from inositol 1,4,5-trisphosphate-insensitive stores [Kuo et al, 2003;Jan et al, 2003].…”