Mechanism of Virus Inactivation by Cold Atmospheric-Pressure Plasma and Plasma-Activated Water

Guo, Li; Xu, Ruobing; Gou, Lu; Liu, Zhichao; Zhao, Yiming; Liu, Dingxin; Zhang, Lei; Chen, Hailan; Kong, Michael G.

doi:10.1128/aem.00726-18

Cited by 201 publications

(185 citation statements)

References 29 publications

(28 reference statements)

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“…It was speculated that a series of chemical reactions occurred in the system of PAW and allantoic fluid system. It has been realized that varieties of ROS in cold plasma could destroy the viral envelope protein and damage the nucleic acid, thus leading to virus inactivation (Guo et al 2018;Morita et al 2000;Su et al 2018;Tagawa et al 2000;Wu et al 2015;Xia et al 2019), which was in a good agreement with our results.…”

Section: Discussionsupporting

confidence: 91%

Preparation of the inactivated Newcastle disease vaccine by plasma activated water and evaluation of its protection efficacy

Zhou¹,

Tian

Xia³

et al. 2019

Appl Microbiol Biotechnol

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Vaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA). In addition, cell-mediated immune response was evaluated by lymphocyte proliferation assay and flow cytometry. The results demonstrated that the vaccine prepared by PAW at appropriate volume ratio could induce similar antibody titers in specific pathogen-free (SPF) chickens compared with the formaldehyde-inactivated vaccine. The challenge experiment further confirmed that the vaccine prepared by PAW conferred solid protection against virulent NDV. Moreover, it was found that the vaccine could promote the proliferation of lymphocytes and stimulate cell-mediated immunity of SPF chickens. Furthermore, analysis of electron spin resonance (ESR) spectroscopy and physicochemical properties of PAW suggested reactive oxygen and nitrogen species (RONS) played an essential role in the virus inactivation. Therefore, this study indicated that NDV treated by PAW in an appropriate ratio retained immunogenicity on the premise of virus inactivation. PAW as a promising strategy could be used to prepare inactivated vaccine for Newcastle disease.

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Section: Discussionsupporting

confidence: 91%

Preparation of the inactivated Newcastle disease vaccine by plasma activated water and evaluation of its protection efficacy

Zhou¹,

Tian

Xia³

et al. 2019

Appl Microbiol Biotechnol

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“…In another study, Guo et al. () reported that both DBD surface discharges and PAW had strong antiviral properties and effectively inactivated the T4, 174, and MS2 bacteriophages in a time‐dependent manner. The PAW exhibited antiviral properties even after 10 days of storage at 22 °C.…”

Section: Microbial Inactivationmentioning

confidence: 99%

“…According to Guo et al. (), the singlet oxygen (O) present in PAW damages the nucleic acid and proteins in all double‐stranded DNA, single‐stranded DNA, and the RNA bacteriophages by aggregation. Zhang et al.…”

Section: Microbial Inactivationmentioning

confidence: 99%

“…They proved this by performing the fatty acid profiling of Escherichia coli cells before and after plasma treatment and reported that there was no significant qualitative change in cell membrane fatty acids. According to Guo et al (2018), the singlet oxygen (O) present in PAW damages the nucleic acid and proteins in all double-stranded DNA, single-stranded DNA, and the RNA bacteriophages by aggregation. Zhang et al (2013) used Ar/O 2 PAW to inactivate Staphylococcus aureus and concluded that the main inactivation mechanism was damage of cell membrane by oxidative stress induced by the reactive species in PAW.…”

Section: Microbial Inactivationmentioning

confidence: 99%

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Nonthermal Plasma–Liquid Interactions in Food Processing: A Review

Perinban

Orsat

Raghavan

2019

Comp Rev Food Sci Food Safe

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Nonthermal processing methods are often preferred over conventional food processing methods to ensure nutritional quality. Nonthermal plasma (NTP) is a new field of nonthermal processing technology and seeing increased interest for application in food preservation. In food applications of NTP, liquid interactions are the most prevalent. The NTP reactivity and product storability are altered during this interaction. The water activated by NTP (plasma‐activated water [PAW]) has gained considerable attention during recent years as a potential disinfectant in fruits and vegetable washing. However, detailed understanding of the interactions of NTP reactive species with food nutritional components in the presence of water and their stability in food is required to be explored to establish the potential of this emerging technology. Hence, the main objective of this review is to give a complete overview of existing NTP–liquid interactions. Further, their microbial inactivation mechanisms and the effects on food quality are discussed in detail. Most of the research findings have suggested the successful application of NTP and PAW for microbial inactivation and food preservation. Still, there are some research gaps identified and a complete analysis of the stability of plasma reactive species in food is still missing. By addressing these issues, along with the available research output in this field, it is possible that NTP can be successfully used as a food decontamination method in the near future.

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“…In addition, several studies reported that one of most inactivation mechanism of plasma is the UV radiation, which caused damage to DNA by inducing the formation of thymine dimmers (29). Moreover, the reactive species generated by plasma, hydrogen peroxide, single oxygen and ozone have strong oxidative effect that cause damage to DNA, as well as peroxynitrite that formed due to the reaction of nitric oxide and superoxide (21,30). baumannii HHR1 isolates.…”

Section: Effect Of Plasma On Dnamentioning

confidence: 99%

Influence of Cold Atmospheric Plasma on Acinetobacter baumannii

al.¹

2019

Baghdad Sci.J

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baumannii is an aerobic gram negative coccobacilli, it is considered multidrug resistance pathogen (MDR) and causes several infections that are difficult to treat. This study is aims to employ physical methods in sterilization and inactivation of A. baumannii, as an alternative way to reduce the using of drugs and antibiotics. Cold Atmospheric Plasma was generated by one electrode at 20KV, 4 power supply and distance between electrode and sample was fixed on 1mm. A. baumannii (ATCC 19704 and HHR1) were exposed to Dielectric Barrier Discharge type of Cold Atmospheric Plasma (DBD-CAP) for several periods of time (15, 30, 45, and 60 sec.) . After sterilization test, several methods were done to analyze the effect of DBD-CAP on bacterial morphology, proteins and DNA. Change in morphology was assessed by cover slid method. Damaged DNA was investigated by PCR technique, and DNA sequencing. The impact of DBD-CAP on the entity of proteins was detected by SDS-PAGE. The observed inactivation of bacterial colony on agar plates has been quantified by measuring the inactivation diameter. The important conclusion that HHR1 more resistance to DBD-CAP than ATCC 17904 because it is more virulence than standard strain; thus, the growth of both strains is largely affected by plasma and this influence is increased by increasing the time of exposure, also the plasma affects the DNA especially on standard strain as it is explained in sequencing result, so it causes more deletion in DNA sequence. In addition, plasma also has been showed to damage proteins and morphology thus, the bacterial cells transform from cocco-bacillus to bacillus.

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Mechanism of Virus Inactivation by Cold Atmospheric-Pressure Plasma and Plasma-Activated Water

Cited by 201 publications

References 29 publications

Preparation of the inactivated Newcastle disease vaccine by plasma activated water and evaluation of its protection efficacy

Preparation of the inactivated Newcastle disease vaccine by plasma activated water and evaluation of its protection efficacy

Nonthermal Plasma–Liquid Interactions in Food Processing: A Review

Influence of Cold Atmospheric Plasma on Acinetobacter baumannii

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