Mechanism of translational regulation by miR-2 from sites in the 5′ untranslated region or the open reading frame

Moretti, Francesca; Thermann, Rolf; Hentze, Matthias W.

doi:10.1261/rna.2384610

Cited by 139 publications

(100 citation statements)

References 35 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This complex binds to the target sequence that is more commonly located within the 3Ј untranslated region (UTR) of the mRNA (10 ). Functional miRNA target sequences in 5Ј UTR and in the open reading frame have also been reported (13 ). The seed region of the miRNA (the first 2-8 nt) is especially important for miRNA target recognition.…”

Section: Function Of Mirnas Mirna Transportation and Signaling Betwmentioning

confidence: 99%

MicroRNAs in the Atherosclerotic Plaque

2013

View full text Add to dashboard Cite

BACKGROUND: MicroRNAs (miRNA, miR) are noncoding RNAs that regulate gene expression by hindering translation. miRNA expression profiles have been shown to differ in vivo and in vitro in many cellular processes associated with cardiovascular diseases (CVDs). The progression of CVDs has also been shown to alter the blood miRNA profile in humans. CONTENT:We summarize the results of animal and cell experiments concerning the miRNA profile in the atherosclerotic process and the changes which occur in the blood miRNA profile of individuals with CVD. We also survey the relationship of these CVD-related miRNAs and their expression in the human advanced atherosclerotic plaque, thereby providing more insight into miRNA function in human atherosclerotic lesions. The miRNAs miR-126, -134, -145, -146a, -198, -210, -340*, and -92a were found to be expressed differently in the blood of individuals affected and unaffected by CVD. These differences paralleled those seen in tissue comparisons of miRNA expression in advanced atherosclerotic plaques and healthy arteries. Furthermore, several miRNAs associated with atherosclerosis in in vitro studies (such as miR-10a, -126, -145, -146a/b, -185, -210, and -326) were expressed in plaques in a similar pattern as was predicted by the in vitro experiments. The clinical implications of miRNAs in atherosclerosis as biomarkers and as possible drug targets are also reviewed.

show abstract

Section: Function Of Mirnas Mirna Transportation and Signaling Betwmentioning

confidence: 99%

MicroRNAs in the Atherosclerotic Plaque

2013

View full text Add to dashboard Cite

show abstract

“…Moreover, even though it is known that microRNAs mainly recognize complementary sequences in the 3' untraslated regions (UTRs) of their target mRNAs, more recent studies have reported that they can also bind to the 5'UTR or the ORF [5][6][7][8] and, even more surprisingly, they can upregulate translation upon growth arrest conditions [9] .…”

Section: Mirnas Biogenesis and Mechanism Of Actionmentioning

confidence: 99%

microRNA: New Players in Metastatic Process

Triulzi¹,

Iorio²,

Tagliabue³

et al. 2013

Oncogene and Cancer - From Bench to Clinic

View full text Add to dashboard Cite

“…7 The mature miRNA is retained in RISC (RNA-induced silencing complex) 8 and it is currently understood that microRNAs mainly bind to the 3' untranslated region (UTR) of their target mRNAs. However, recent studies have reported that microRNAs do not only bind to 3'UTR but also to 5'UTR 9,10 or open reading frame (ORF) 11,12 of the target mRNA. By binding to the 3'UTRs, 5'UTR or ORF of target mRNAs, microRNAs regulate the translation of proteins from mRNA or degrade the mRNA itself.…”

Section: What Is Microrna?mentioning

confidence: 99%

Role of microRNAs in solid tumors

Hamano¹,

Ishii²,

Miyata³

et al. 2011

J Nucleic Acids Invest

View full text Add to dashboard Cite

Accumulating experimental evidence indicates that microRNAs play important roles in various biological processes, such as cell differentiation, proliferation, metabolism and apoptosis. In addition, several reports concluded that altered expression of specific microRNA genes contributes to the initiation and progression of cancer. Here, we summarize the current knowledge about aberrant expression of various microRNAs in human solid cancers (e.g., lung, breast, and gastric cancers), their target proteins, and the relationship between their expression and response to chemotherapies. We also review the potential for using microRNAs as biomarkers for the diagnosis and cancer therapy. The development of treatment strategies against human solid cancers based on the profile and/or certain features of microRNAs is promising. What is microRNA?MicroRNAs are noncoding, single-stranded RNAs, 18-25 nucleotides long, and were first reported in Caenorhabditis elegans in 1993. 1 Subsequent studies led to the identification of microRNAs in human RNA, 2 as well as to the understanding of their mechanisms of action. Most human miRNAs are found within introns of either protein-coding or noncoding mRNA transcripts, 3 and they do not code for any protein although they are RNA sequences.MicroRNA genes are generally transcribed by RNA polymerase II in the nucleus to form pri-miRNA transcripts. These are processed into pre-miRNAs by a microprocessor complex, which contains the Rnase III enzyme Drosha 4 and DGCR8.5 Exportin5 and a RanGTP 6 transport the pre-miRNAs from the nucleus to the cytoplasm, where they are further processed by the RNAase III enzyme Dicer. 7 The mature miRNA is retained in RISC (RNA-induced silencing complex) 8 and it is currently understood that microRNAs mainly bind to the 3' untranslated region (UTR) of their target mRNAs. However, recent studies have reported that microRNAs do not only bind to 3'UTR but also to 5'UTR 9,10 or open reading frame (ORF) 11,12 of the target mRNA. By binding to the 3'UTRs, 5'UTR or ORF of target mRNAs, microRNAs regulate the translation of proteins from mRNA or degrade the mRNA itself. 13While microRNAs are thought to repress the translation of target mRNAs, recent results demonstrated that microRNAs can activate the expression of the target genes.14 In the same study, microRNA was reported to be essential for translation activation under growth arrest conditions. Regulation of translation by microRNAs might change from repression to activation depending on the cell cycle.In addition, because microRNA can bind even to mRNA that is not partially complementary, 15 microRNA and mRNA do not correspond one-to-one, 16 such that one microRNA may regulate several mRNAs or one mRNA may be regulated by several microRNAs. For example, in human gliomas, miR-34a inhibits the expression of multiple oncogenes (e.g., c-Met, Notch-1/Notch-2 and CDK6) by binding to their 3'-UTR and suppressing tumor growth.17 Thus, these microRNAs potentially regulate approximately 30% of all genes encoding h...

show abstract

Mechanism of translational regulation by miR-2 from sites in the 5′ untranslated region or the open reading frame

Cited by 139 publications

References 35 publications

MicroRNAs in the Atherosclerotic Plaque

MicroRNAs in the Atherosclerotic Plaque

microRNA: New Players in Metastatic Process

Role of microRNAs in solid tumors

Contact Info

Product

Resources

About