Mechanism of the radiation-induced degradation of nucleic acids

Scholes, G.; Ward, John F.; Weiß, Joseph

doi:10.1016/s0022-2836(60)80049-6

Cited by 219 publications

(30 citation statements)

References 18 publications

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“…with the de.oxypentose moieties (Scholes et al 1960). Evidence is accumulating, however, which is consistent with the notion that the proportion of attack on the bases as compared with that on the pentose in native double stranded DNA is less until after the molecule has become partially uncoiled as a result of stran* breakage (Ward 1975).…”

Section: Oh (2-6)supporting

confidence: 60%

“…The reaction prevents s~ from reacting with Thy (or DNA) but 'OH aq can still add to give the Thy-'OH adduct radical. Oxygen adds to this radical to give the 5-hydroxy-6-hydroperoxy radical (Scholes et al,, 1960) …”

Section: Addition Of Water or Parts Of Watermentioning

confidence: 99%

“…This accumulated evidence suggests that further investigation of attachment and related reactions which may be reasonably expected to occur in cells will be an important and critical part of our continuing efforts to understand radiation effects and develop ways to modify them. Teoule, et al, 1972Infante, et al, 1973Smith and Hays, 1968Scholes et al, 1960Karael and Garrison, 1965Kamel and Garrison, 1965Brown, et al, 1966 it Zarebska andShugar, 1972 Elad, et al, 1969 ti Steinmaus, et al, 1969Leonov, et al, 1975 …”

Section: N-ethylmalimide (Nem)mentioning

confidence: 99%

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Ionizing Radiation-Induced Attachment Reactions of Nucleic Acids and Their Components

Myers

1976

Aging, Carcinogenesis, and Radiation Biology

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Section: Oh (2-6)supporting

confidence: 60%

Section: Addition Of Water or Parts Of Watermentioning

confidence: 99%

Section: N-ethylmalimide (Nem)mentioning

confidence: 99%

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Ionizing Radiation-Induced Attachment Reactions of Nucleic Acids and Their Components

Myers

1976

Aging, Carcinogenesis, and Radiation Biology

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“…The creation of structural diversity may be especially important in primary tumor formation and metastasis in that it sets the stage for the selection of malignant DNA phenotypes. It has been estimated that the rate of attack of the ⅐OH on the nucleotide bases is about five times that for deoxyribose (22). Also, the DNAs from normal (RMT) tissue and metastatic tumor tissue have been shown to have substantial structural diversity (4,5), at least partly as a result of ⅐OH-induced modifications in the nucleotide bases and the phosphodiester-deoxyribose structure.…”

Section: Discussionmentioning

confidence: 99%

Tumor progression to the metastatic state involves structural modifications in DNA markedly different from those associated with primary tumor formation

Malins

Polissar

Gunselman

1996

Proc. Natl. Acad. Sci. U.S.A.

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Wavenumber-absorbance relationships of infrared spectra of DNA analyzed by principal components analysis may be expressed as points in space. Each point represents a highly discriminating measure of DNA structure. Structural modifications of DNA, such as those induced by free radicals, alter vibrational and rotational motion and consequently change the spatial location of the points. Using this technology to analyze breast tumor DNA, we revealed a 94؇ difference in direction between the progression of normal DNA 3 primary tumor DNA and the progression of primary tumor DNA 3 metastatic tumor DNA (P < 0.001). This sharp directional change was accompanied by a substantial increase in the structural diversity of the metastatic tumor DNA (P ‫؍‬ 0.003), which, on the basis of the volume of the core cluster of points, could comprise as many as 11 ؋ 10 9 different phenotypes. This suggests that the heterogeneity and varied physiological properties known to characterize malignant tumor cell populations may at least partially arise from these diverse phenotypes. The evidence suggests that the progression to the metastatic state involves structural modifications in DNA that are markedly different from the modifications associated with the formation of the primary tumor. Overall, the findings of this and earlier studies imply that the observed DNA alterations are a pivotal factor in the etiology of breast cancer and a formidable barrier to overcome in intervention to control the disease. In terms of cancer etiology and prediction, the technology described has potentially wide application to studies in which the structural status of DNA is an important consideration.A significant body of evidence points to the involvement of the hydroxyl radical (⅐OH) in introducing mutagenic structures into DNA of the normal female breast, thus statistically increasing the probability of breast cancer (1-5). In a recent study (5), the transformation of primary breast tumors to the metastatic state was shown to involve a Ͼ2-fold increase in ⅐OH damage in DNA, as indicated by modified nucleotide base models comprising mutagenic 8-hydroxyadenine (6) and the putatively nonmutagenic ring-opened product 4,6-diamino-5-formamidopyrimidine (fapyadenine; refs. 7-11). In addition, plots of the modified nucleotide base model log 10 (fapyadenine͞8-hydroxyadenine) versus the size of metastatic and nonmetastatic breast tumors revealed that the metastatic tumor DNA had significantly greater structural diversity than the nonmetastatic tumor DNA (P ϭ 0.01; ref. 5).Principal components analysis (PCA) of data obtained by Fourier transform-infrared (FT-IR) spectroscopy yielded plots in which individual spectra were represented as points in twoor three-dimensional space. Each point was a highly discriminating representation of an individual DNA structure in that spatially and visually close points had Ͻ3% average spectral difference over the range 1750-700 cm Ϫ1 (5). The core cluster of metastatic tumor DNA points was substantially larger than the core c...

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“…These reactivity has been extensively studied in the context of hydroxyl radical (HO•), which is generated by radiolysis of water. When DNA is exposed to the hydroxyl radical, approximately 80% of the reactions occur at the bases [12][13][14]. Many base damage products arising from the reaction hydroxyl radical with DNA have been characterized [13,[15][16][17][18][19].…”

Section: Reactions Of Radicals With the Heterocyclic Basesmentioning

confidence: 99%

Degradation of nucleic acids and nucleotides in several conditions with perspectives of retrieval: A review

Reyes

Orozco²,

Jaramillo³

et al. 2014

ABB

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Deoxyribonucleic acid (DNA) or oligonucleotides, can be modified in several ways as chemical degradation by electrophilic reaction, attack of radicals, hydrolytic deamination or oxidative damage caused by ionizing radiation. This work discussed these degradation mechanisms, determining the effects on these biomolecules. The actual knowledge about DNA damages only permits partial enzymatic repair treatments.

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Mechanism of the radiation-induced degradation of nucleic acids

Cited by 219 publications

References 18 publications

Ionizing Radiation-Induced Attachment Reactions of Nucleic Acids and Their Components

Ionizing Radiation-Induced Attachment Reactions of Nucleic Acids and Their Components

Tumor progression to the metastatic state involves structural modifications in DNA markedly different from those associated with primary tumor formation

Degradation of nucleic acids and nucleotides in several conditions with perspectives of retrieval: A review

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