Mechanism of the combined anti-bacterial effect of green tea extract and NaCl against Staphylococcus aureus and Escherichia coli O157:H7

Nakayama, Motokazu; Shigemune, Naofumi; Tsugukuni, Takashi; Hitomi, Jun; Matsushita, Taku; Mekada, Yoko; Kurahachi, Masahiro; Miyamoto, Takahisa

doi:10.1016/j.foodcont.2011.10.021

Cited by 47 publications

(30 citation statements)

References 24 publications

(19 reference statements)

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“…17,18) Because the cells were treated with EGCg in a short time, we think that the protein expression was almost unchanged in those cells. However, the level of the protein expression after EGCg treatment needs to be examined.…”

Section: Discussionmentioning

confidence: 99%

Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis

Nakayama

Shimatani

Ozawa

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Catechins are a class of polyphenols and have high anti-bacterial activity against various microorganisms. Here, we report the mechanism for antibacterial activity of epigallocatechin gallate (EGCg) against Gram-positive bacteria Bacillus subtilis, which is highly sensitive to EGCg. Transmission electron microscope analysis revealed that deposits containing EGCg were found throughout the cell envelope from the outermost surface to the outer surface of cytoplasmic membrane. Aggregating forms of proteins and EGCg were identified as spots that disappeared or showed markedly decreased intensity after the treatment with EGCg compared to the control by two-dimensional electrophoresis. Among the identified proteins included 4 cell surface proteins, such as oligopeptide ABC transporter binding lipoprotein, glucose phosphotransferase system transporter protein, phosphate ABC transporter substrate-binding protein, and penicillin-binding protein 5. Observations of glucose uptake of cells and cell shape B. subtilis after the treatment with EGCg suggested that EGCg inhibits the major functions of these proteins, leading to growth inhibition of B. subtilis.

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Section: Discussionmentioning

confidence: 99%

Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis

Nakayama

Shimatani

Ozawa

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…13 In a study by Nakayama et al, 49 the mechanism of the combined antibacterial effect of green tea extract (GTE) and NaCl against Stapylococcus aureus NBRC 13276 and Escherichia coli O157:H7 was investigated. Among catechins, galloylated catechins such as EGCG and ECG were found to be more active than non-galloylated catechins against bacteria.…”

Section: Tea Catechins and Their Chemistrymentioning

confidence: 99%

Catechin prodrugs and analogs: a new array of chemical entities with improved pharmacological and pharmacokinetic properties

et al. 2013

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Extensive research on tea catechins, mainly (-)-epigallocatechin gallate, has shown numerous health promoting effects. However, various clinical studies demonstrated several issues associated with tea catechins which account for their poor systemic bioavailability. In order to improve pharmacological activity and bioavailability of natural tea catechins, two major strategies have been adopted to date which include synthesizing catechin analogs/prodrugs and the development of novel drug delivery systems. In this review, we provide a detailed account of novel synthetic analogs/prodrugs as well as novel drug delivery approaches used for natural tea catechins to make them therapeutically potent drug-like molecules.

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“…Possibly, this higher activity is due to the presence of polyphenols present in GTGE. Several authors have demonstrated high effective antibacterial plant extracts containing polyphenols (Guedes et al, 2009;Rotava et al, 2009 (Nakayama et al, 2012). These catechins are the key ingredients of green tea and have various functionalities, including anti-bacterial action (Chan et al, 2011).…”

Section: Determination Of Antibacterial Activitymentioning

confidence: 99%

Antibacterial Activity of Gels with Pomegranate, Apricot and Green Tea Glycolic Extracts

Calixto¹

2012

J. App. Pharm. Sci.

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Pomegranate (PGE) and green tea (GTGE) glycolic extracts are being employed in formulations because of their antiseptic and astringent effects. Apricot (AGE) glycolic extract possesses function cooling and antibacterial. The aim was to verify the antibacterial activity of these extracts incorporated in gel base. The antibacterial activity was verified by diffusion in agar method, using cylinder in plate. Plates containing Staphylococcus aureus (ATCC 6538p), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 10536) and Salmonella sp. (ATCC 19196) were incubated at 37ºC for 24 hours. After incubation, the results were analysed with a pachymeter, observing the bacterial growth inhibition halo diameter and the statistical significance level was determined. PGE presented activity only against P. aeruginosa; GTGE presented activity against S. aureus, P. aeruginosa and E. coli; and AGE presented activity against P. aeruginosa and Salmonella sp. According to the experimental conditions, it is possible to conclude that GTGE presented the greater growth inhibition halo diameter when compared with other extracts, suggesting higher antibacterial action of this extract.

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Mechanism of the combined anti-bacterial effect of green tea extract and NaCl against Staphylococcus aureus and Escherichia coli O157:H7

Cited by 47 publications

References 24 publications

Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis

Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis

Catechin prodrugs and analogs: a new array of chemical entities with improved pharmacological and pharmacokinetic properties

Antibacterial Activity of Gels with Pomegranate, Apricot and Green Tea Glycolic Extracts

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