Mechanism of the antiviral effect of hydroxytyrosol on influenza virus appears to involve morphological change of the virus

Yamada, Kentaro; Ogawa, Hideoki; Hara, Ayako; Yoshida, Yukio; Yonezawa, Yutaka; Karibe, Kazuji; Nghia, Vuong Bui; Yoshimura, Hidetoshi; Yamamoto, Yu; Yamada, Manabu; Nakamura, K; Imai, Kunitoshi

doi:10.1016/j.antiviral.2009.03.002

Cited by 75 publications

(67 citation statements)

References 24 publications

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“…Thereby, it is conceivable that the inhibitory effect of PGG was mainly caused by the direct interaction of PGG with virus. Numerous studies have demonstrated that plant polyphenols including the tea catechins (-) epigallocatechin gallate (EGCG) and theaflavin digallate [34], resveratrol (RV) [26], a polyphenol rich extract (CYSTUS052) [11], pomegranate polyphenol extract (PPE) [13] , oligonol [12], and Hydroxytyrosol (HT) [38], have potent antiviral activity against influenza virus, that are related to the nature of their interactions with viral particles. Additionally, indirect effects of some polyphenols, such as EGCG and strictinin, on host cells that might interfere with virus-cell membrane fusion have also been suggested [23,32].…”

Section: Discussionmentioning

confidence: 99%

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

et al. 2011

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Influenza A virus (IAV) infection is a major public health threat leading to significant morbidity and mortality. The emergence of drug-resistant virus strains highlights urgent needs to develop novel antiviral drugs with alternative modes of action. Pentagalloyl glucose (PGG), a natural occuring polyphenolic compound, possesses broad spectrum of biological activities. In this study, we found PGG has anti-influenza virus activity and investigated its possible mechanism(s) of action in vitro.

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Section: Discussionmentioning

confidence: 99%

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

et al. 2011

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“…Therefore, inhibition of viral propagation was not performed throughout the HA inhibition and it suggests other mechanisms such as structural damage to the virion can be responsible for the direct antiviral activity of the MOEO. The inactivation of different influenza virus subtypes like as H9N2 by direct exposure to hydroxytyrosol, a phenolic derivative of the leaves and fruits of olive were associated with damage to virion integrity, but HA and NA functions were unaffected [33]. Furthermore, it has been shown that quercetin 3-rhamnoside inhibits virus replication in the initial stage of virus infection by indirect interaction with virus particles [6].…”

Section: Discussionmentioning

confidence: 99%

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

et al. 2016

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Lemon balm derivatives are going to acquire a novelty as natural and potent remedy for treatment of viral infections since the influenza viruses are developing resistance to the current antivirals widely. Oseltamivir, Melissa officinalis essential oil (MOEO) and their synergistic efficacy against avian influenza virus (AIV) subtype H9N2 were evaluated in vitro in MDCK cells at different time exposure by using TCID50, HA, Real Time PCR and HI assay. The results showed that MOEO could inhibit replication of AVI through the different virus replication phase (P B 0.05). Also the highest antiviral activity of MOEO was seen when AIV incubated with MOEO before cell infection. The TCID50/ml was reduced 1.3-2.1, 2.3-2.8, 3.7-4.5 log 10 than control group (5.6 log 10), HAU/50 ll was decreased 85-94, 71.4-94, 71.4-94 % and viral genome copy number/ll was brought down 68-95, 90-100, 89.6-99.9 % at pre-infection, post-infection and simultaneous stage, respectively. Hemagglutination inhibition result showed the MOEO was not able to inhibit agglutination of the chicken red blood cell (cRBC). Replication of the AVI was suppressed by the different concentration of oseltamivir completely or near 100 %. Also oseltamivir showed a synergistic activity with MOEO especially when oseltamivir concentration reduced under 0.005 mg/ml. The chemical composition was examined by GC-MS analysis and Its main constituents were identified as monoterpenaldehydes citral a, citral b. In conclusion, the findings of the study showed that lemon balm essential oil could inhibit influenza virus replication through different replication cycle steps especially throughout the direct interaction with the virus particles.

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“…The use of olive oil in the diet is believed to be associated with a lower incidence of cancer and cardiovascular diseases in the Mediterranean population (Fabiani et al 2008;Visioli et al 1998). Potential benefits of hydroxytyrosol (HT) are also suggested for preventing diabetes, vascular damage, and influenza (Jemai et al 2009;Jemai et al 2008;Yamada et al 2009). A recent study showed activation of longevity proteins (SIRT and FoxO) in heart cells of HT-fed rats, suggesting that HT could impact ageing (Mukherjee et al 2009).…”

Section: Introductionmentioning

confidence: 99%

MnSOD activity regulates hydroxytyrosol-induced extension of chronological lifespan

Sarsour

Kumar

Kalen

et al. 2011

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Chronological lifespan (CLS) is defined as the duration of quiescence in which normal cells retain the capacity to reenter the proliferative cycle. This study investigates whether hydroxytyrosol (HT), a naturally occurring polyphenol found in olives, extends CLS in normal human fibroblasts (NHFs). Quiescent NHFs cultured for a long duration (30-60 days) lose their capacity to repopulate. Approximately 60% of these cells exit the cell cycle permanently; a significant increase in the doubling time of the cell population was observed. CLS was extended in quiescent NHFs that were cultured in the presence of HT for 30-60 days. HT-induced extension of CLS was associated with an approximately 3-fold increase in manganese superoxide dismutase (MnSOD) activity while there was no change in copper-zinc superoxide dismutase, catalase, or glutathione peroxidase protein levels. Quiescent NHFs overexpressing a dominant-negative mutant form of MnSOD failed to extend CLS. HT suppressed ageassociated increase in mitochondrial ROS levels. Results from spectroscopy assays indicate that HT in the presence of peroxidases can undergo catechol-semiquinone-quinone redox cycling generating superoxide, which in a cellular context can activate the antioxidant system, e.g., MnSOD expression. These results demonstrate that HT extends CLS by increasing MnSOD activity and decreasing age-associated mitochondrial reactive oxygen species accumulation.

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Mechanism of the antiviral effect of hydroxytyrosol on influenza virus appears to involve morphological change of the virus

Cited by 75 publications

References 24 publications

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

MnSOD activity regulates hydroxytyrosol-induced extension of chronological lifespan

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