Mechanism of <scp>DYRK1a</scp> in myocardial ischemia–reperfusion injury by regulating ferroptosis of cardiomyocytes

Wang, Jing; Xu, Rui‐Ming; Cao, Qiu‐Mei; Ma, Bing‐Chen; Zhang, Hao; Hao, Hua‐Peng

doi:10.1002/kjm2.12753

Cited by 2 publications

(2 citation statements)

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“…4B). Considering the important role of TGEV-induced apoptosis in host response to TGEV infection (Wang et al 2022), the expression patterns of differentially expressed miRNAs involved in apoptosis were analyzed. Among them, the expression of miR-181 markedly reduced after PCP and TGEV infection, and the result was also confirmed by Real-time PCR (P < 0.001) (Fig.…”

Section: High-throughput Sequencing Analysismentioning

confidence: 99%

“…Due to the low efficacy, cytotoxicity, and viral resistance of synthetic antiviral drugs such as Moroxidine, Ribavirin, and Ganciclovir (Märtson et al 2022, Yu et al 2016, natural antiviral drugs are considered easy to accept because of their low toxicity and low price (Ghosh et al 2009). PCP has been proven to have pharmacological effects such as antiviral, antibacterial, anti-inflammatory, and anti-tumor effects (Wang et al 2022). Therefore, we conducted TGEV challenge on weaned piglets and then treated them with PCP.…”

Section: Discusionmentioning

confidence: 99%

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Polygonum Cillinervepolysaccharide inhibits Transmissible gastroenteritis virus by regulating microRNA-181

Duan,

Li,

Tan

et al. 2023

Preprint

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Transmissible gastroenteritis virus (TGEV) is an important pathogen that can cause changes in the expression profile of cell miRNA. In this study, PCP could treat piglets infected with TGEV by in vivo experiment. High-throughput sequencing technology was used to detect that 9 miRNAs were up-regulated and 17 miRNAs were down-regulated during PCP inhibition of TGEV infection in PK15 cells. Meanwhile, miR-181 was related to the target genes of key proteins of apoptosis pathway. PK15 was treated with different PCP after transfection of miR-181 mimic or inhibitor. Real-time PCR was used to detect the effect of TGEV replication, electron microscopy, TEM and Hoechst fluorescence staining were used to detect the biological function of the treated cells, and western blot (WB) was used to detect the expression of key signaling factors cyt C, caspase 9 and P53 in the apoptotic signaling pathway. The results showed that compared with the control group, 250 μg/mL PCP significantly inhibited the replication of TGEV gRNA and gene N (P< 0.01). PK15 cells in PCP groups (250 and 125 μg/mL) had uniform cell morphology and a small number of floating cells by microscopic, no typical virus structure was observed in 250 μg/mL PCP group under TEM, and apoptosis staining showed that 250 μg/mL PCP significantly reduced the number of apoptotic cells. PCP may inhibit TGEV-induced apoptosis through Caspase-dependent mitochondrial pathway after transfection of miR-181. The above results provided a theoretical basis for further research on the mechanism of PCP anti-TGEV.

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Section: High-throughput Sequencing Analysismentioning

confidence: 99%