2006
DOI: 10.1038/sj.emboj.7601260
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Mechanism of RecA-mediated homologous recombination revisited by single molecule nanomanipulation

Abstract: The mechanisms of RecA-mediated three-strand homologous recombination are investigated at the singlemolecule level, using magnetic tweezers. Probing the mechanical response of DNA molecules and nucleoprotein filaments in tension and in torsion allows a monitoring of the progression of the exchange in real time, both from the point of view of the RecA-bound single-stranded DNA and from that of the naked double-stranded DNA (dsDNA). We show that strand exchange is able to generate torsion even along a molecule w… Show more

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Cited by 39 publications
(42 citation statements)
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“…The resulting 'overstretched' form of the molecule is approximately 1.7 times longer than helical B-DNA. Overstretching is of crucial importance for the biological function of DNA: the bacterial protein RecA elongates DNA by a factor of 1.5 upon binding [3,4,5], a mechanism central to homologous recombination and to chromosomal segregation during cell division [6]. However, the nature of the overstretched state remains a source of considerable controversy: some think overstretched DNA in vitro to be a hybridized form called S-DNA (the 'Bto-S' picture) [7,8,9,10,11,12], while a competing picture considers overstretching to signal a conversion to unhybrizided single strands (the 'force-melting' picture) [13,14,15,16,17,18].…”
Section: Introductionmentioning
confidence: 99%
“…The resulting 'overstretched' form of the molecule is approximately 1.7 times longer than helical B-DNA. Overstretching is of crucial importance for the biological function of DNA: the bacterial protein RecA elongates DNA by a factor of 1.5 upon binding [3,4,5], a mechanism central to homologous recombination and to chromosomal segregation during cell division [6]. However, the nature of the overstretched state remains a source of considerable controversy: some think overstretched DNA in vitro to be a hybridized form called S-DNA (the 'Bto-S' picture) [7,8,9,10,11,12], while a competing picture considers overstretching to signal a conversion to unhybrizided single strands (the 'force-melting' picture) [13,14,15,16,17,18].…”
Section: Introductionmentioning
confidence: 99%
“…Biophysical Journal 00(00) [1][2][3][4][5][6][7][8][9][10][11][12] These equations can be solved, producing from Eq. (7)…”
Section: Theoretical Modelmentioning
confidence: 99%
“…During this process a nucleotide sequence of another identical or similar DNA duplex is used for restoring the original sequence in the broken DNA molecule (8,9). Furthermore, the homologous recombination plays a fundamental role in the process of genetic diversity and reassembling of genetic information, which is important for living systems for adaption to changing environments (1,3,5,8,(11)(12)(13).…”
Section: Introductionmentioning
confidence: 99%
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“…Recent work by Fulconis et al 63 utilized the changes in DNA length upon RecA coating to monitor strand-exchange reactions in real time. The authors used magnetic tweezers to stretch RecA-coated single-stranded DNA and monitored the exchange with a strand of a duplex DNA molecule.…”
Section: Reca Filament Formation On Dnamentioning
confidence: 99%