Mechanism of Protection by Anionic Detergents against Denaturation of Serum Albumin

Solubilization and Properties of the Apoproteins of the Very Low- and Low-Density Lipoproteins of Human Serum*

“…Addition of SDS would prevent such aggregation by favoring nonpolar interactions between the protein and the detergent. Such a process has been shown to occur between SDS and serum albumin by Karush and Sonnenberg (1950) and is probably partially responsible for the protective action of SDS against denaturation of albumin by urea (Markus et al, 1964). In our studies either nonionic (Triton) or cationic (benzalkonium chloride) detergents failed to exhibit the solubilizing effect of the anionic SDS.…”

Surface-Active Ionic Liquid Cholinium Dodecylbenzenesulfonate: Self-Assembling Behavior and Interaction with Cellulase

“…Variations in the intrinsic fluorescence of cellulase have been examined at various concentrations of Cho­[DBS] at excitation (λ ex )/emission (λ em ) wavelengths of 280 nm/340 nm. , Intrinsic fluorescence of cellulase appeared because of the fluorophore Trp, Phe, and Tyr domains present in the microenvironment of the protein. Cellulase absorbs ultraviolet light around 280 and 230 nm because of the n−π* transitions in aromatic amino acid residues and the π–π* transitions in the protein backbone, respectively. , The effect coming from the absorption and emission of pure Cho­[DBS] is negligible as compared to cellulase. Changes in the fluorescence intensity are indicated in Figure A.…”

“…28 A sequential discussion has been made by the results obtained from various complementary techniques. 33,46 The effect coming from the absorption and emission of pure Cho[DBS] is negligible as compared to cellulase. Changes in the fluorescence intensity are indicated in Figure 7A.…”

The Interaction of Bovine Plasma Albumin with Detergent Anions. Stoichiometry and Mechanism of Binding of Alkylbenzenesulfonates*

“…Various observations that low levels of detergent ions protect albumins toward denaturation have been attributed to this effect (Foster, 1960). More recent observations show that low levels of DSrepress the high pH expansion of BPA (Lovrien, 1963) and the urea denaturation of human plasma albumin (Markus et al, 1964). In the latter case the detergent binding isotherms actually exhibited a sigmoidal character in the presence of 6 m urea at alkaline pH.…”