Mechanism of proline uptake by Chlorella vulgaris

Selection and Characterization of Chlorella Mutants Deficient in Amino Acid Transport

“…Acridine orange was purchased from Fluka (18). Some have also been used in studies with plant cells (1,6,9,17). To employ them for selection of transport mutants, it is necessary that the corresponding analogs are toxic for the cells under investigation.…”

“…To employ them for selection of transport mutants, it is necessary that the corresponding analogs are toxic for the cells under investigation. To select mutants of Chlorella deficient in L-arginine and L-proline uptake, L-canavanine (for L-arginine) and five competitive inhibitors of proline uptake (pyrrole-2 carboxylic acid, L-azetidine-2-carboxylic acid, thioazolidine-2-carboxylic acid [1], N-methyl-L-leucine, and sarcosine [N. Sauer, unpublished data]) were tested for toxicity. Growth of Chlorella cells on plates with minimal medium containing Dglucose and one of the analogs was followed over a 10-d period, and the results are listed in Table II.…”

Transporters for nitrogenous compounds in plants

“…Arginine transport in sugarcane suspension culture cells occurs in the fully ionized state and is mediated by electrogenic uniport [53]. Analysis of the neutral amino acid transport system from Chlorella indicates that the transport occurs in the zwitterionic form by proton symport [ 14]. Electrophysiological data have been presented for different mechanisms driving transport of amino acids.…”

Extracellular deamination of l-amino acids by Chlamydomonas reinhardtii cells

“…This takes place either by means of energy-dependent transport systems (Kirk and Kirk 1978a, b;Cho et al 1981;Cho and Komor 1983;Flynn and Syrett 1985;Sauer and Tanner 1985) or by deamination mechanisms occurring at the cell surface with the subsequent uptake and assimilation of the ammonium produced Cooksey 1979, 1981a, b).…”