Mechanism of mitochondrial DNA replication in mouse L cells: Localization of alkali-sensitive sites at the two origins of replication

Margolin, Kim; Doda, Jackie N.; Clayton, David A.

doi:10.1016/0147-619x(81)90041-x

Cited by 12 publications

(3 citation statements)

References 21 publications

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“…The presence of depurinated sites and ribonucleotides which are found in mammalian mtDNAs (32,33), if present in plant mtDNA, would make not only the larger DNA molecules but even some of the smaller circular species particularly fragile. Therefore it is not unexpected that isolated mtDNA consists of a series of fragmented, linear molecules, with a low proportion of circular DNA species.…”

Section: Stability Of Plant Mitochondrial Dnamentioning

confidence: 99%

A review of the structure and organization of the mitochondrial genome of higher plants

Lonsdale

1984

Plant Mol Biol

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The structure, organisation and functions of the mitochondrial genome of most groups of eukaryotic organisms are known to varying degrees with the notable exception of higher plants. With the recent completion of physical mapping studies on the Brassica campestris (Chinese cabbage, turnip) and Zea mays (maize) mitochondrial genomes, many of the apparent problems of plant mitochondrial genome structure can now be answered. In this manuscript I review the literature relating to the physical observations on plant mitochondrial DNA (mtDNA) and assess the data in relation to our current understanding of mitochondrial genome structure.

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Section: Stability Of Plant Mitochondrial Dnamentioning

confidence: 99%

A review of the structure and organization of the mitochondrial genome of higher plants

Lonsdale

1984

Plant Mol Biol

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“…Biochemical and genetic evidence favour a model where mtDNA is replicated via a strand displacement mechanism with continuous DNA synthesis on both strands, although other models for mtDNA replication have been suggested ( 20 – 22 ). H-strand DNA replication is initiated at O H and continues to displace the parental H-strand ( 4 , 22 , 23 ). During this first phase of replication, there is no simultaneous DNA synthesis on the L-strand.…”

Section: Introductionmentioning

confidence: 99%

Regulation of DNA replication at the end of the mitochondrial D-loop involves the helicase TWINKLE and a conserved sequence element

et al. 2015

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The majority of mitochondrial DNA replication events are terminated prematurely. The nascent DNA remains stably associated with the template, forming a triple-stranded displacement loop (D-loop) structure. However, the function of the D-loop region of the mitochondrial genome remains poorly understood. Using a comparative genomics approach we here identify two closely related 15 nt sequence motifs of the D-loop, strongly conserved among vertebrates. One motif is at the D-loop 5′-end and is part of the conserved sequence block 1 (CSB1). The other motif, here denoted coreTAS, is at the D-loop 3′-end. Both these sequences may prevent transcription across the D-loop region, since light and heavy strand transcription is terminated at CSB1 and coreTAS, respectively. Interestingly, the replication of the nascent D-loop strand, occurring in a direction opposite to that of heavy strand transcription, is also terminated at coreTAS, suggesting that coreTAS is involved in termination of both transcription and replication. Finally, we demonstrate that the loading of the helicase TWINKLE at coreTAS is reversible, implying that this site is a crucial component of a switch between D-loop formation and full-length mitochondrial DNA replication.

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“…Three main modes were proposed for the replication of the human mitochondrial DNA: the Strand Displacement Model (SDM) ( 5–14 ), the RNA Incorporated ThroughOut the Lagging Strand (RITOLS) ( 15–22 ) model, and the Strand Coupled Mechanism (SCM) ( 15 , 23 ) (Figure 1 ). The relative frequency of in vivo utilization of these three mechanisms, their regulations, and reciprocal inter-connections remain mainly unknown.…”

Section: Introductionmentioning

confidence: 99%

A rapid method to visualize human mitochondrial DNA replication through rotary shadowing and transmission electron microscopy

Kosar

Piccini

Foiani

et al. 2021

Nucleic Acids Research

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We report a rapid experimental procedure based on high-density in vivo psoralen inter-strand DNA cross-linking coupled to spreading of naked purified DNA, positive staining, low-angle rotary shadowing, and transmission electron microscopy (TEM) that allows quick visualization of the dynamic of heavy strand (HS) and light strand (LS) human mitochondrial DNA replication. Replication maps built on linearized mitochondrial genomes and optimized rotary shadowing conditions enable clear visualization of the progression of the mitochondrial DNA synthesis and visualization of replication intermediates carrying long single-strand DNA stretches. One variant of this technique, called denaturing spreading, allowed the inspection of the fine chromatin structure of the mitochondrial genome and was applied to visualize the in vivo three-strand DNA structure of the human mitochondrial D-loop intermediate with unprecedented clarity.

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Mechanism of mitochondrial DNA replication in mouse L cells: Localization of alkali-sensitive sites at the two origins of replication

Cited by 12 publications

References 21 publications

A review of the structure and organization of the mitochondrial genome of higher plants

A review of the structure and organization of the mitochondrial genome of higher plants

Regulation of DNA replication at the end of the mitochondrial D-loop involves the helicase TWINKLE and a conserved sequence element

A rapid method to visualize human mitochondrial DNA replication through rotary shadowing and transmission electron microscopy

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