Mechanism of miR-148b inhibiting cell proliferation and migration of Schwann cells by regulating CALR

Abstract

The aim of this study is to investigate the effect of miR-148b on cell proliferation and migration of Schwann cells and explore its mechanism. The miR-148b group, miR-con group and the anti-miR-148b group, anti-miR-con group, si-con group, si-CALR group, Ctrl group, CALR group were transfected into Schwann cells by liposome method; the expression of miR-148b was detected by qRT-PCR; the cell viability was detected by MTT assay; the migration of cells was detected by Transwell method; WB assay was used to detect the protein expression of CALR. Firstly, we found that compared with miR-con group and si-con group, the proliferation and migration of miR-148b group and si-CALR group were significantly down-regulated (P < .05). Moreover, compared with anti-miR-con group and Ctrl group, anti-miR-148b group and CALR group cells proliferation and migration were significantly up-regulated (P < .05). In addition, miR-148b was targeted to CALR, and silencing CALR could reverse the inhibitory effect of miR-148b on Schwann cell proliferation and migration. In conclusion, miR-148b can regulate the proliferation and migration of Schwann cells. The mechanism may be related to the targeted negative regulation of CALR, which will provide a basis for targeted therapy of peripheral nerve injury.

Bone Marrow Derived Cells Mediated Regulation of Schwann Cells Proliferation and Migration In Vitro

“…Previous study has indicated numerous methods that could promote the proliferation and migration of SCs [35][36][37] . However, these methods frequently involve in complex, di cult to be popularized, and what is more, these factors cannot enhance SC proliferation in the early stage by one hand and further promote SC migration in the late stage by the other hand.…”

Bone Marrow Derived Cells Mediated Regulation of Schwann Cells Proliferation and Migration In vitro

“…Previous study has indicated numerous methods that could promote the proliferation and migration of SCs [35][36][37]. However, these methods frequently involve in complex, difficult to be popularized, and what is more, these factors cannot enhance SC proliferation in the early stage by one hand and further promote SC migration in the late stage by the other hand.…”

Role of Non-coding RNAs in Axon Regeneration after Peripheral Nerve Injury

“…These findings suggest a possible approach for the treatment of PNI. In addition, miR-34a and miR-148b overexpression evidently inhibited the proliferation and migration of SCs by targeting contactin-2 and calreticulin, respectively 60 , 61 . At the same time, a large number of miRNAs have been shown to promote the proliferation and migration of SCs.…”