Mechanism of liver regeneration after partial hepatectomy using mouse cDNA microarray

Togo, Shinji; Makino, Hirochika; Kobayashi, Takatoshi; Morita, Tomoyuki; Shimizu, Tetsuya; Kubota, Takeshi; Ichikawa, Yasushi; Ishikawa, Takashi; Okazaki, Yasushi; Hayashizaki, Yoshihide; Shimada, Hiroshi

doi:10.1016/j.jhep.2003.11.005

Cited by 58 publications

(58 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genes such as Igfbp1, LCN2, and Saa2 are upregulated, whereas Car3, Serpina6, and Cyp2f2 are down-regulated ( Fig. 1C,D ;Togo et al 2004;White et al 2005).…”

Section: Histone H2a Ubiquitylation Correlates With Repressed Genes Dmentioning

confidence: 99%

“…These programs include hormone biosynthesis, lipid and amino acid metabolisms, and nucleotide biosynthesis. The transcription network revealed by microarray expression profiling during hepatocyte regeneration indicated that Igfbp1, LCN2, and Saa2 increase but, in contrast, Car3, Serpina6 (also called CBG; corticosteroid-binding globulin), and Cyp2f2 are manifestly decreased ∼12 h after hepatectomy (Togo et al 2004;White et al 2005). These genes are also important for hepatic regeneration, in terms of homeostasis and hepatic metabolism.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation

Nakagawa

Kajitani²,

Togo³

et al. 2008

Genes Dev.

Self Cite

200

226

View full text Add to dashboard Cite

Transcriptional initiation is a key step in the control of mRNA synthesis and is intimately related to chromatin structure and histone modification. Here, we show that the ubiquitylation of H2A (ubH2A) correlates with silent chromatin and regulates transcriptional initiation. The levels of ubH2A vary during hepatocyte regeneration, and based on microarray expression data from regenerating liver, we identified USP21, a ubiquitin-specific protease that catalyzes the hydrolysis of ubH2A. When chromatin is assembled in vitro, ubH2A, but not H2A, specifically represses the di-and trimethylation of H3K4. USP21 relieves this ubH2A-specific repression. In addition, in vitro transcription analysis revealed that ubH2A represses transcriptional initiation, but not transcriptional elongation, by inhibiting H3K4 methylation. Notably, ubH2A-mediated repression was not observed when H3 Lys 4 was changed to arginine. Furthermore, overexpression of USP21 in the liver up-regulates a gene that is normally down-regulated during hepatocyte regeneration. Our studies revealed a novel mode of trans-histone cross-talk, in which H2A ubiquitylation controls the di-and trimethylation of H3K4, resulting in regulation of transcriptional initiation.[Keywords: Nucleosome; ubiquitylation; histone code; transcription; USP21; H2A] Supplemental material is available at http://www.genesdev.org.

show abstract

“…Genes such as Igfbp1, LCN2, and Saa2 are upregulated, whereas Car3, Serpina6, and Cyp2f2 are down-regulated ( Fig. 1C,D ;Togo et al 2004;White et al 2005).…”

Section: Histone H2a Ubiquitylation Correlates With Repressed Genes Dmentioning

confidence: 99%

mentioning

confidence: 99%

Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation

Nakagawa

Kajitani²,

Togo³

et al. 2008

Genes Dev.

Self Cite

200

226

View full text Add to dashboard Cite

show abstract

“…These transcription factors subsequently activate immediate-early growth genes including myc, fos and jun that have been identified in mouse genetic models and more recently through the use of expression profiling with microarrays. 19,[47][48][49][50] Mitogen activated protein kinases including extracellular-regulated signal kinase (ERK) 51 and Jun Kinase (JNK) are also rapidly activated after partial hepatectomy. Although hepatocyte specific deletion of the HGF receptor, Met, is associated with loss of ERK activation and reduced hepatocyte proliferation posthepatectomy, the downstream targets of ERK have not yet been identified.…”

Section: The Partial Hepatectomy Model Is a Powerful Tool For The Idementioning

confidence: 99%

Cell cycle regulation and hepatocarcinogenesis

Greenbaum¹

2004

Cancer Biology & Therapy

View full text Add to dashboard Cite

“…Four measurement time points were adopted; 0, 24, 72 and 168 h. There are some reports of microarray analyses related to liver regeneration, but most have been measured in the early phases of regeneration (26,(32)(33)(34)(35). Because our concern is more specific to the changes in the ABC transporters, we analyzed the later phase according to the data obtained in the previous report (20).…”

Section: Discussionmentioning

confidence: 99%

Gene expression of ATP-binding cassette transporters during liver regeneration after 90% hepatectomy in rats

et al. 2012

View full text Add to dashboard Cite

Abstract. Liver damage with hyperbilirubinemia during regeneration of the small liver is the major hurdle to expand the indications of adult living donor liver transplantation. We performed a large-scale gene expression analysis of the regenerating liver after a 90% hepatectomy in rats, and analyzed the changes in the gene expression patterns related to the ATP-binding cassette (ABC) transporters. RNAs were prepared from 3 rat livers at 0, 24, 72 and 168 h after a 90% hepatectomy. The gene expression profile was analyzed by the Rat Genome 230 2.0 array with special references to the ABC transporters. Among 31,042 probes, 1,587 reported genes were identified as either upregulated or downregulated more than 2-fold. Among 20 ABC transporter genes, multidrug resistance protein (MRP) 2 and organic anion transporting polypeptide (OATP) 1 were significantly downregulated, while MRP1 and MRP3 tended to be expressed. These genetic changes were confirmed by real-time PCR. A microarray analysis demonstrated not only an extensive gene expression profile in the regenerating liver but more specific molecular events related to bilirubin transport at the same time. Changes in the expression pattern of the ABC transporters, therefore, seem to be the key event in liver failure during liver regeneration.

show abstract

Mechanism of liver regeneration after partial hepatectomy using mouse cDNA microarray

Cited by 58 publications

References 22 publications

Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation

Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation

Cell cycle regulation and hepatocarcinogenesis

Gene expression of ATP-binding cassette transporters during liver regeneration after 90% hepatectomy in rats

Contact Info

Product

Resources

About