Mechanism of Inactivation of Oral Cancer Cells Irradiated by Active Oxygen Species from DBD Plasma

Mine, Keisuke; Miyamaru, Yukie; Hayashi, Nobuya; Aijima, Reona; Yamashita, Yoshio

doi:10.1615/plasmamed.2017019246

Cited by 6 publications

(11 citation statements)

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“…Direct; air-oxygen torch-type DBD In vitro HSC3 Mine et al [29] Physical plasma exposure generates a large amount of oxidative stress in oral cancer cells and has a significant inhibitory effect on cancer cell growth due to the promoted activation of caspases, a compromised redox state, and a loss of MMP that results in the decline of cellular viability.…”

Section: Indirect; Dc-powered Air-water Plasma Jetmentioning

confidence: 99%

“…Most of these experimental in vitro studies used the oral squamous cell carcinoma cell line SCC-15, as well as SCC-25, and highlighted their plasma-induced inactivation due to the reduction of cell growth, induction of apoptosis, and secondary DNA damage, especially through plasma-derived ROS [19,25,28,[34][35][36][37][38]. Other HNSCC cell lines were also tested, showing one or more of the tumor-toxic plasma-associated characteristics [6,19,22,24,[26][27][28][29][30][31][32][33]37]. In addition, HNSCC has been shown to be more prone to physical plasma treatment than non-malignant cells since the viability of the latter did not significantly decrease after plasma application.…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…In addition, HNSCC has been shown to be more prone to physical plasma treatment than non-malignant cells since the viability of the latter did not significantly decrease after plasma application. The fact that plasma selectively targets cancer cells, leaving non-malignant cell types, such as human fibroblasts (e.g., human gingival fibroblast-1 (HGF-1), HS-K, and IMR-SV-90) or human keratinocytes (HaCaT) unaffected, makes plasma a promising tool for adjuvant head and neck cancer therapy [25,27,29]. Moreover, two studies demonstrated an enhanced selectivity of plasma after the addition of antibody-conjugated gold nanoparticles, which specifically targeted SCC-25 head and neck cancer cells [35,36].…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…Besides EGFR, studies suggested a crucial role of the p53-signaling pathway in plasma-induced head and neck cancer cell death [23,29,33,37]. This protein contributes to the redox balance within the cells via regulation of the antioxidant gene expression and further senses DNA damage, initiates signaling pathways for the DNA repair, and fulfills the role of a cell-cycle regulator [20].…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…Moreover, Hayashi et al and Mine et al demonstrated the contribution of p53 during plasma-induced apoptosis. They showed that plasma-mediated activation of mitogen-activated protein kinase (MAPK) proteins, like c-Jun N-terminal kinase (JNK) and p38, induces p53 phosphorylation, which resulted in apoptosis of the HNSCC cell line HSC3 [29,33].…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

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Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

et al. 2020

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Despite progress in oncotherapy, cancer is still among the deadliest diseases in the Western world, emphasizing the demand for novel treatment avenues. Cold physical plasma has shown antitumor activity in experimental models of, e.g., glioblastoma, colorectal cancer, breast carcinoma, osteosarcoma, bladder cancer, and melanoma in vitro and in vivo. In addition, clinical case reports have demonstrated that physical plasma reduces the microbial contamination of severely infected tumor wounds and ulcerations, as is often seen with head and neck cancer patients. These antimicrobial and antitumor killing properties make physical plasma a promising tool for the treatment of head and neck cancer. Moreover, this type of cancer is easily accessible from the outside, facilitating the possibility of several rounds of topical gas plasma treatment of the same patient. Gas plasma treatment of head and neck cancer induces diverse effects via the deposition of a plethora of reactive oxygen and nitrogen species that mediate redox-biochemical processes, and ultimately, selective cancer cell death. The main advantage of medical gas plasma treatment in oncology is the lack of adverse events and significant side effects compared to other treatment modalities, such as surgical approaches, chemotherapeutics, and radiotherapy, making plasma treatment an attractive strategy for the adjuvant and palliative treatment of head and neck cancer. This review outlines the state of the art and progress in investigating physical plasma as a novel treatment modality in the therapy of head and neck squamous cell carcinoma.

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Section: Indirect; Dc-powered Air-water Plasma Jetmentioning

confidence: 99%

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

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Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

et al. 2020

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Chronic oxidative stress adaptation in head and neck cancer cells generates slow-cyclers with decreased tumour growth in vivo

et al. 2023

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Background Reactive oxygen species (ROS) are implicated in cancer therapy and as drivers of microenvironmental tumour cell adaptations. Medical gas plasma is a multi-ROS generating technology that has been shown effective for palliative tumour control in head and neck cancer (HNC) patients before tumour cells adapted to the oxidative stress and growth regressed fatally. Methods In a bedside-to-bench approach, we sought to explore the oxidative stress adaptation in two human squamous cell carcinoma cell lines. Gas plasma was utilised as a putative therapeutic agent and chronic oxidative stress inducer. Results Cellular responses of single and multiple treated cells were compared regarding sensitivity, cellular senescence, redox state and cytokine release. Whole transcriptome analysis revealed a strong correlation of cancer cell adaption with increased interleukin 1 receptor type 2 (IL1R2) expression. Using magnetic resonance imaging, tumour growth and gas plasma treatment responses of wild-type (WT) and repeatedly exposed (RE) A431 cells were further investigated in a xenograft model in vivo. RE cells generated significantly smaller tumours with suppressed inflammatory secretion profiles and increased epidermal growth factor receptor (EGFR) activity showing significantly lower gas plasma sensitivity until day 8. Conclusions Clinically, combination treatments together with cetuximab, an EGFR inhibitor, may overcome acquired oxidative stress resistance in HNC.

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The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro

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Pawłat

Terebun

et al. 2019

J. Phys. D: Appl. Phys.

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The aim of this work was to evaluate the impact of atmospheric pressure nitrogen plasma on viability, proliferation, and osteogenic differentiation of normal mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4), which were maintained in Hanks’ balanced salt solution (HBSS) during plasma exposure. Obtained results clearly demonstrated that short-time (4, 8, and 16 s) nitrogen plasma treatment is non-toxic to the MC3T3-E1 cells, does not affect cell morphology, promotes preosteoblasts’ proliferation, enhances osteogenic differentiation by increasing bone alkaline phosphatase and osteocalcin concentration, but inhibits mineralization of extracellular matrix. The best results were achieved for 16 s exposure time and when the preosteoblasts were left in HBSS for 3 h after plasma treatment. Presented studies indicate great clinical potential of cold atmospheric nitrogen plasma for regenerative medicine applications to improve bone healing process.

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Mechanism of Inactivation of Oral Cancer Cells Irradiated by Active Oxygen Species from DBD Plasma

Cited by 6 publications

References 0 publications

Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

Medical Gas Plasma Treatment in Head and Neck Cancer—Challenges and Opportunities

Chronic oxidative stress adaptation in head and neck cancer cells generates slow-cyclers with decreased tumour growth in vivo

The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro

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