Mechanism of hypersensitive cell collapse: correlation of increased lipoxygenase activity with membrane damage in leaves of Phaseolus vulgaris (L) inoculated with an avirulent race of Pseudomonas syringae pv. phaseolicola

Croft, K. P. C.; Voisey, Christine R.; Slusarenko, Alan

doi:10.1016/0885-5765(90)90091-b

Cited by 139 publications

(71 citation statements)

References 36 publications

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“…17 In particular, hydrogen peroxide has been shown to be a crucial component of the HR control circuit, since a short pulse of exogenous H 2 O 2 is sufficient to activate the hypersensitive cell death program in soybean cells. 17 A similar HR has been observed also in bean, 16 pepper 18 and tobacco 19 leaves, infected with different pathogens. Interestingly, LOX activation has been associated to HR, 18,19 and H 2 O 2 oxidative stress has been shown to induce animal cell apoptosis, through activation of 5-LOX.…”

Section: Plant Lipoxygenases and Apoptosissupporting

confidence: 49%

Lipoxygenases and their involvement in programmed cell death

2001

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Lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids, thus initiating lipoperoxidation of membranes and the synthesis of signaling molecules. Consequently, they induce structural and metabolic changes in the cell in a number of pathophysiological conditions. Recently, a pro-apoptotic effect of lipoxygenase, and of the hydroperoxides produced thereof, has been reported in different cells and tissues, leading to cell death. Anti-apoptotic effects of lipoxygenases have also been reported; however, this has often been based on the use of enzyme inhibitors. Here we review the characteristics of the lipoxygenase family and its involvement in the initiation of oxidative stress-induced apoptosis. Finally, we discuss the role of lipoxygenase activation in apoptosis of animal and plant cells, suggesting a common signal transduction pathway in cell death conserved through evolution of both kingdoms. Cell Death and Differentiation (2001) 8, 776 ± 784.

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Section: Plant Lipoxygenases and Apoptosissupporting

confidence: 49%

Lipoxygenases and their involvement in programmed cell death

2001

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“…A Abbreviations: AM, arbuscular mycorrhiza; AMF arbuscular mycorrhizal fungi; APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; GST, glutathione S-transferase; HR, hypersensitive reaction; ROS, reactive oxygen species; SOD, superoxide dismutase. (Auh & Murphy, 1995;Bolwell et al, 1995;Croft et al, 1990;Doke, 1983;Doke & Miura, 1995;Levine et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…A variety of enzyme systems have been proposed to generate ROS in plants. These include a neutrophil-analogous, membrane-bound NADPH oxidase, a lipoxygenase and apoplastic peroxidases (Auh & Murphy, 1995;Bolwell et al, 1995;Croft et al, 1990;Doke, 1983;Doke & Miura, 1995;Levine et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant enzyme activities in maize plants colonized with Piriformospora indica

et al. 2009

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The bioprotection performance of Piriformospora indica against the root parasite Fusarium verticillioides was studied. We found that maize plants first grown with F. verticillioides and at day 10 inoculated with P. indica showed improvements in biomass, and root length and number as compared with plants grown with F. verticillioides alone. To validate our finding that inoculation with P. indica suppresses colonization by F. verticillioides, we performed PCR analyses using P. indica-and F. verticillioides-specific primers. Our results showed that inoculation with P. indica suppresses further colonization by F. verticillioides. We hypothesized that as the colonization by P. indica increases, the presence of/colonization by F. verticillioides decreases. In roots, catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST) and superoxide dismutase (SOD) activities were found to be higher in F. verticillioides-colonized plants than in noncolonized plants. Increased activity of antioxidant enzymes minimizes the chances of oxidative burst (excessive production of reactive oxygen species), and therefore F. verticillioides might be protected from the oxidative defence system during colonization. We also observed decreased antioxidant enzyme activities in plants first inoculated with F. verticillioides and at day 10 inoculated with P. indica as compared with plants inoculated with F. verticillioides alone. These decreased antioxidant enzyme activities due to the presence of P. indica help the plant to overcome the disease load of F. verticillioides. We propose that P. indica can be used as a bioprotection agent against the root parasite F. verticillioides. INTRODUCTIONMycorrhizal associations are among the most widespread, intimate and important symbioses in terrestrial ecosystems. Among these, arbuscular mycorrhiza (AM) represent the most ancient and widespread mycorrhizal symbiosis, and are found in a wide range of land plant species (Harrison, 1997). The successful establishment of this mutualistic association constitutes a strategy to fulfil the nutritional demands of both partners . This requires a balance between the defence responses of the host plant and the nutrient demands of the endophyte, resulting in an altered defence-related gene expression, which has been extensively studied during host colonization by obligate biotrophic arbuscular mycorrhizal fungi (AMF). Induction of defence-related genes is most prominent during the early stages of colonization (García-Garrido & Ocampo, 2002), but can also be detected during arbuscule development (Grunwald et al., 2004).The active resistance of plants to colonization by fungi is often expressed by the hypersensitive reaction (HR) of challenged plant cells, and is characterized by reactive oxygen species (ROS), as well as by induced rapid and localized death of plant tissue at the site of infection (Ingram, 1978;Tenhaken et al., 1995). At the biochemical level, the rapid generation of ROS, such as superoxide (O { 2 ) and hydrogen peroxide (H 2 O 2 ), kno...

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“…pisi infection ofcucumber cotyledons. Croft et al (7) reported that the increase in lipoxygenase activity preceded ethane evolution (a measure oflipid peroxidation) during P. syringae pv. syringae (an avirulent race) infection of Phaseolus vulgaris.…”

Section: Discussionmentioning

confidence: 99%

Use of Dimethyl Sulfoxide to Detect Hydroxyl Radical during Bacteria-Induced Hypersensitive Reaction

Popham¹,

Novacký²

1991

Plant Physiol.

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Excess active oxygen is generated during the hypersensitive reaction (HR), an incompatible reaction of plants to bacterial pathogens. During HR, lipid peroxidation correlates chronologically with production of the oxygen species, superoxide (02-). However MO) seedlings were grown in a growth chamber at 23 to 270C with a photoperiod of 14 h/l0 h light/ dark. The seedlings were grown in vermiculite in plastic trays with cheesecloth covering aeration holes in the bottom. The plants were watered daily from below with 1 mM CaSO4. On days 3 and 5, the CaSO4 was replaced with a nutrient solution consisting of the following: 4 mm CaCl2 2H20, 0.45 mM K2HPO4.3H20, 0.5 mM MgSO4.7H20, 1.25 mM K2SO4, 10 mM NH4NO3, 26 AM Fe citrate, 2.3 AM H3BO3, 0.9 ,AM MnSO4. H20, 0.6 ,uM ZnSO4.7H20, 0.15 ,M CuSO4.5H20, 0.1 M Na2MoO4. 2H20, 0.01 ,uM CoCl2 * 6H20, and 0.1 1 AM NiCl2 * 6H20 (D.G. Blevins, Department of Agronomy, University of Missouri, personal communication). Experiments were performed on 9-to 1 -d-old plants. BacteriaPseudomonas syringae pv. pisi, a bacterium that induces HR on cucumber, was used for all experiments. The bacteria were stored at -70TC in 12% glycerol. When needed, bacteria were removed from the freezer and streaked onto a nutrient agar plate. The bacteria from the plate were transferred to a nutrient agar slant 48 h before inoculation and then were transferred from the slant to nutrient broth 12 to 18 h before inoculation. Bacteria were incubated at 25°C on a reciprocal shaker to obtain a high concentration of cells in the log phase of growth. Preceding inoculation, bacteria were collected by centrifugation at l0,OOOg for 5 min. The bacteria were resuspended in distilled water and collected by centrifugation at l0,OOOg for 5 min. The bacteria were again resuspended in distilled water, and the suspension was adjusted spectrophotometrically to 108 colony forming units mL-'. Plants were inoculated by piercing the abaxial epidermis of the cotyledons 1157 www.plantphysiol.org on April 3, 2019 -Published by Downloaded from

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Mechanism of hypersensitive cell collapse: correlation of increased lipoxygenase activity with membrane damage in leaves of Phaseolus vulgaris (L) inoculated with an avirulent race of Pseudomonas syringae pv. phaseolicola

Cited by 139 publications

References 36 publications

Lipoxygenases and their involvement in programmed cell death

Lipoxygenases and their involvement in programmed cell death

Antioxidant enzyme activities in maize plants colonized with Piriformospora indica

Use of Dimethyl Sulfoxide to Detect Hydroxyl Radical during Bacteria-Induced Hypersensitive Reaction

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