Mechanism of H2-electrooxidation with immobilized hydrogenase

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

“…[83,84] Whereas in the late 80s, electrochemistry provided essential kinetic data for Hases and their physiological partners, [85][86][87][88] Yoropolov's group, followed by Hagen's then Armstrong's groups demonstrated that Hases could yield a direct current for H 2 oxidation or proton reduction when adsorbed at graphite electrodes. [83,84,88,89] The influences of parameters such as pH, temperature, gas composition, and graphite polishing procedure were evaluated, [89][90][91] before fundamental research was undertaken to understanding of the catalytic properties of this exciting enzyme. Essential kinetic data were then available to support the spectroscopic data.…”

Stable ‘Floating' Air Diffusion Biocathode Based on Direct Electron Transfer Reactions Between Carbon Particles and High Redox Potential Laccase

“…Thus, in the case of O 2 electrocatalytic reduction, high-power densities limited only by O 2 diffusion to the electrode surface can be obtained. Based on information available from previous investigations regarding enzyme stability and enzyme capacity [25][26][27]29], two carbon materials were selected, coal BM-4 and carbon black PM-100.…”

“…For more than 30 years, Russian electrochemists have been studying O 2 bioelectroreduction by Lcs adsorbed on different electrode materials [6,7,[25][26][27][28][29]. In many cases bioelectrochemical results from the Russian school are presented as polarisation curves with positive cathodic currents and potentials expressed versus the NHE, which causes some difficulties for other scientists to compare results thus gathered with data already available.…”

“…The main physical-chemical and electrochemical properties of these materials can be found in Refs. [25,26,30,31]. Usage of particulate carbonaceous materials with particle sizes varying from 4 up to 1000 nm and above (the average size of Lc is ca.…”

“…For comparison, the maximal surface concentration of electrochemically active Lc on planar carbon electrodes is estimated to be about 2 pmol cm -2 [13]. Basic properties of biocatalysts based on Lc adsorbed on dispersed carbon materials were studied and some results were published in Russian journals [25][26][27], including the very first report on DET-derived BFCs based on carbon particles [29]. As early as 2002, suggestions for exploitation of 'air diffusion electrodes, which ensure high overall current density in a biofuel cell' were given.…”