Mechanism of gene transfection by polyamidoamine (PAMAM) dendrimers modified with ornithine residues

Kumar, Ajay; Yellepeddi, Venkata K.; Vangara, Kiran Kumar; Strychar, Kevin B.; Palakurthi, Srinath

doi:10.3109/1061186x.2011.568061

Cited by 9 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the use of higher dendrimer excess likely leads to the decrease of the particle size [34,41,42], with the size evolution profiles correlating with those of zeta potential. This factor is important for the efficient cell internalization of dendriplexes, since it occurs by means of clathrin- or caveolin-mediated endocytosis [43,44], where the size of nanoparticles to be internalized is limited. Summarizing, the use of CR sat /MR sat obtained from the zeta potential profiles for the preparation of dendriplexes is likely preferential for the biological experiments (at least, in vitro).…”

Section: Resultsmentioning

confidence: 99%

Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

et al. 2019

View full text Add to dashboard Cite

This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both “easy-to-transfect” cells (HeLa) and “hard-to transfect” ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Cytotoxicity of SN-38-SBEβCD complex on ovarian cancer cell lines A2780 and 2008 was evaluated by MTT assay (27)(28)(29) and compared with the cytotoxicity of SN-38 and irinotecan. As SN-38 is not soluble in water, a stock solution was made in DMSO and suitable dilutions were made in growth medium, keeping the final DMSO concentration less than 0.1%.…”

Section: In Vitro Cytotoxicity Studiesmentioning

confidence: 99%

SN-38-Cyclodextrin Complexation and Its Influence on the Solubility, Stability, and In Vitro Anticancer Activity Against Ovarian Cancer

Vangara

Ali

et al. 2014

AAPS PharmSciTech

Self Cite

View full text Add to dashboard Cite

Abstract. SN-38, an active metabolite of irinotecan, is up to 1,000-fold more potent than irinotecan. But the clinical use of SN-38 is limited by its extreme hydrophobicity and instability at physiological pH. To enhance solubility and stability, SN-38 was complexed with different cyclodextrins (CDs), namely, sodium sulfobutylether β-cyclodextrin (SBEβCD), hydroxypropyl β-cyclodextrin, randomly methylated β-cyclodextrin, and methyl β-cyclodextrin, and their influence on SN-38 solubility, stability, and in vitro cytotoxicity was studied against ovarian cancer cell lines (A2780 and 2008). Phase solubility studies were conducted to understand the pattern of SN-38 solubilization. SN-38-βCD complexes were characterized by differential scanning calorimetry (DSC), X-ray powder diffraction analysis (XRPD), and Fourier transform infrared (FTIR). Stability of SN-38-SBEβCD complex in pH 7.4 phosphate-buffered saline was evaluated and compared against free SN-38. Phase solubility studies revealed that SN-38 solubility increased linearly as a function of CD concentration and the linearity was characteristic of an A P -type system. Aqueous solubility of SN-38 was enhanced by about 30-1,400 times by CD complexation. DSC, XRPD, and FTIR studies confirmed the formation of inclusion complexes, and stability studies revealed that cyclodextrin complexation significantly increased the hydrolytic stability of SN-38 at physiological pH 7.4. Cytotoxicity of SN-38-SBEβCD complex was significantly higher than SN-38 and irinotecan in both A2780 and 2008 cell lines. Results suggest that SBEβCD encapsulated SN-38 deep into the cavity forming stable inclusion complex and as a result increased the solubility, stability, and cytotoxicity of SN-38. It may be concluded that preparation of inclusion complexes with SBEβCD is a suitable approach to overcome the solubility and stability problems of SN-38 for future clinical applications.

show abstract

“…(a) MTT assay The cytotoxicity of G1.0, G2.0, G3.0, and G4.0 PAMAM-NH 2 to the human gastric epithelial cell line GES-1 was determined by a standard MTT assay (24,25). Briefly, cells were maintained in DMEM media with 10% fetal bovine serum (FBS) and 30 μg/mL gentamicin.…”

Section: Toxicity Assaysmentioning

confidence: 99%

Amino-Terminated Generation 2 Poly(amidoamine) Dendrimer as a Potential Broad-Spectrum, Nonresistance-Inducing Antibacterial Agent

Xue

Chen

Mao

et al. 2012

AAPS J

View full text Add to dashboard Cite

Abstract. The treatment of septicemia caused by antibiotic-resistant bacteria is a great challenge in the clinic. Because traditional antibiotics inevitably induce bacterial resistance, which is responsible for many treatment failures, there is an urgent need to develop novel antibiotic drugs. Amino-terminated Poly (amidoamine) dendrimers (PAMAM-NH 2 ) are reported to have antibacterial activities. However, previous studies focused on high generations of PAMAM-NH 2 , which have been found to exhibit high toxicities. The present study aimed to clarify whether low generations of PAMAM-NH 2 could be used as novel antibacterial agents. We found that generation 2 (G2.0) PAMAM-NH 2 showed significant antibacterial effects against antibiotic-sensitive and antibiotic-resistant strains but exhibited little toxicity to human gastric epithelial cells and did not induce antibiotic resistance in bacteria. Scanning and transmission electron microscopy analyses suggested that G2.0 PAMAM-NH 2 might inhibit the growth of bacteria by destroying their cell membranes. The administration of G2.0 PAMAM-NH 2 dosedependently improved the animal survival rate of mice infected with extended-spectrum beta lactamase-producing Escherichia coli (ESBL-EC) and of animals infected with a combination of ESBL-EC and methicillin-resistant Staphylococcus aureus. A treatment regimen of 10 mg/kg of G2.0 PAMAM-NH 2 starting 12 h before inoculation followed by 10 mg/kg at 0.5 h after inoculation rescued 100% of singly infected mice and 60% of multiply infected mice. The protective effects were associated with the reduction of the bacterial titers in the blood and with the morphological amelioration of infected tissues. These findings demonstrate that the G2.0 PAMAM-NH 2 is a potential broad-spectrum and nonresistance-inducing antibiotic agent with relatively low toxicity.

show abstract

Mechanism of gene transfection by polyamidoamine (PAMAM) dendrimers modified with ornithine residues

Cited by 9 publications

References 45 publications

Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

SN-38-Cyclodextrin Complexation and Its Influence on the Solubility, Stability, and In Vitro Anticancer Activity Against Ovarian Cancer

Amino-Terminated Generation 2 Poly(amidoamine) Dendrimer as a Potential Broad-Spectrum, Nonresistance-Inducing Antibacterial Agent

Contact Info

Product

Resources

About