Mechanism of E. coli Inactivation by Direct-in-liquid Electrical Discharge Plasma in Low Conductivity Solutions

Estifaee, Pooya; Su, Xukun; Yannam, Sudheer Kumar; Rogers, Shane; Thagard, Selma Mededovic

doi:10.1038/s41598-019-38838-7

Cited by 37 publications

(14 citation statements)

References 51 publications

(55 reference statements)

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“…Error bars represent standard error of the mean by a smaller change in pH compared to Milli-Q. Previous research has also demonstrated that in situ plasma treatment elicits physical conditions on microbes, including electric fields (Estifaee et al, 2019) and ultraviolet radiation (Zheng, 2017), that can further contribute to antimicrobial power. Further research is required to fully understand what causes the biocidal effects of BSD-PAW in situ.…”

Section: Discussionmentioning

confidence: 99%

The antimicrobial efficacy of plasma-activated water against Listeria and E. coli is modulated by reactor design and water composition

Rothwell

Alam

Carter

et al. 2022

Journal of Applied Microbiology

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Aims: This study aimed to compare the efficacy of plasma-activated water (PAW) generated by two novel plasma reactors against pathogenic foodborne illness organisms. Methods and results:The antimicrobial efficacy of PAW produced by a bubble spark discharge (BSD) reactor and a dielectric barrier discharge-diffuser (DBDD) reactor operating at atmospheric conditions with air, multiple discharge frequencies and Milli-Q and tap water, was investigated with model organisms Listeria innocua and Escherichia coli in situ. Optimal conditions were subsequently employed for pathogenic bacteria Listeria monocytogenes, E. coli and Salmonella enterica. DBDD-PAW reduced more than 6-log of bacteria within 1 min. The BSD-PAW, while attaining high log reduction, was less effective. Analysis of physicochemical properties revealed that BSD-PAW had a greater variety of reactive species than DBDD-PAW.Scavenger assays designed to specifically sequester reactive species demonstrated a critical role of superoxide, particularly in DBDD-PAW.Conclusions: DBDD-PAW demonstrated rapid antimicrobial activity against pathogenic bacteria, with superoxide the critical reactive species. Significance and impact of study: This study demonstrates the potential of DBDD-PAW produced using tap water and air as a feasible and cost-effective option for antimicrobial applications, including food safety.

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Section: Discussionmentioning

confidence: 99%

The antimicrobial efficacy of plasma-activated water against Listeria and E. coli is modulated by reactor design and water composition

Rothwell

Alam

Carter

et al. 2022

Journal of Applied Microbiology

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“…Other reactive species that could not be elucidated in this study, or synergy between components of the complex mixture present in BSD-PAW, may have been responsible for its bactericidal activity. In addition, recent studies suggest that plasma treatment elicits physical conditions, including electric fields (30), that were not investigated here. Further research is required to fully understand what causes the biocidal effects of BSD-PAW.…”

Section: Discussionmentioning

confidence: 99%

The Antimicrobial Efficacy Of Plasma Activated Water Is Modulated By Reactor Design And Water Composition

Alam

Carter

et al. 2021

Preprint

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Plasma activated water (PAW) contains a cocktail of reactive oxidative species and free radicals and has demonstrated efficacy as a sanitizer for fresh produce, however there is a need for further optimization. The antimicrobial efficacy of PAW produced by a bubble spark discharge (BSD) reactor and a dielectric barrier discharge-diffuser (DBDD) reactor operating at atmospheric conditions with air, discharge frequencies of 500, 1000 and 1500 Hz, and MilliQ and tap water, was investigated with model organisms Listeria innocua and Escherichia coli. Optimal conditions were subsequently employed for pathogenic bacteria Listeria monocytogenes, E. coli and Salmonella enterica. PAW generated with the DBDD reactor decontaminated more than 6-log CFU of bacteria within 1 minute of treatment. The BSD-PAW, while attaining high CFU reduction was less effective, particularly for L. innocua. Analysis of physicochemical properties revealed BSD-PAW had a greater variety of reactive species than DBDD-PAW. Scavenger assays were employed to specifically sequester reactive species, including the short-lived superoxide (?O2-) radical that could not be directly measured in the PAW. This demonstrated a critical role of superoxide for the inactivation of both E. coli and L. innocua by DBDD-PAW, while in BSD-PAW it had a role in L. innocua inactivation only. Overall, this study demonstrates the potential of DBDD-PAW in fresh produce, where there is a need for sterilization while minimizing chemical inputs and residues and maintaining food quality. Highly effective PAW was generated using air as a processing gas and tap water, making this a feasible and cost-effective option.

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“…In addition, chemical species derived from the air plasma indicates a better result compared to the chemical species from Ar plasma ( Cubas et al, 2021 ). Then, the upper electrode of the gas phase that discharges close to the water surface indicates the high damage of bacteria, followed by reactive species’ attack ( Estifaee, 2019 ). Thus, the inactivation of bacteria can be obtained by plasma technology.…”

Section: Degradation Of Contaminants In Plasma Technologymentioning

confidence: 99%

Degradation of contaminants in plasma technology: An overview

Sanito

You

Wang

2022

Journal of Hazardous Materials

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The information of plasma technologies applications for environmental clean-up on treating and degrading metals, metalloids, dyes, biomass, antibiotics, pesticides, volatile organic compounds (VOCs), bacteria, virus and fungi is compiled and organized in the review article. Different reactor configurations of plasma technology have been applied for reactive species generation, responsible for the pollutants removal, hydrogen and methane production and microorganism inactivation. Therefore, in this review article, the reactive species from discharge plasma are presented here to provide the insight into the environmental applications. The combinations of plasma technology with flux agent and photocatalytic are also given in this review paper associated with the setup of the plasma system on the removal process of metals, VOCs, and microorganisms. Furthermore, the potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inactivation via plasma technology is also described in this review paper. Detailed information of plasma parameter configuration is given to support the influence of the critical process in the plasma system to deal with contaminants.

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Mechanism of E. coli Inactivation by Direct-in-liquid Electrical Discharge Plasma in Low Conductivity Solutions

Cited by 37 publications

References 51 publications

The antimicrobial efficacy of plasma-activated water against Listeria and E. coli is modulated by reactor design and water composition

The antimicrobial efficacy of plasma-activated water against Listeria and E. coli is modulated by reactor design and water composition

The Antimicrobial Efficacy Of Plasma Activated Water Is Modulated By Reactor Design And Water Composition

Degradation of contaminants in plasma technology: An overview

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