Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach

Hao, Min; Ji, De; Lin, Li; Su, Lianlin; Gu, Wei; Gu, Liya; Wang, Qiaohan; Lu, Tu-Lin; Mao, Chunqin

doi:10.3390/molecules24010082

Cited by 32 publications

(29 citation statements)

References 54 publications

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“…The detailed information of disordered metabolic pathways is presented in . In this study, the pathway impact value and P value were set to 0.10 and 0.01 according to previous researches [31, 37, 38]. 5 of the metabolic pathways (steroid hormone biosynthesis, linoleic acid metabolism, alpha-linolenic acid metabolism, retinol metabolism, and arachidonic acid metabolism) showed evident changes in BSS rats according to impact >0.1 and P < 0.01, which indicated they might be significantly associated with BSS's development and RWCI intervention.…”

Section: Resultsmentioning

confidence: 99%

Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS

Ran

Pang

Guan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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In traditional Chinese medicine theory, blood stasis syndrome (BSS), characterized by blood flow retardation and blood stagnation, is one of the main pathologic mechanisms and clinical syndromes of cardiovascular diseases (CVDs). Rhodiola wallichiana var. cholaensis injection (RWCI) is made from dry roots and stems of RWC via the processes of decoction, alcohol precipitation, filtration, and dilution. Studies indicated the extracts of RWC could alleviate CVDs; however, the mechanism had not been illustrated. In the present study, the acute blood stasis rat model was established to investigate the pathogenesis of BSS and the therapeutic mechanism of RWCI against BSS. Hemorheological parameters (whole blood viscosity and plasma viscosity) and inflammatory factors (TNF-α and IL-6) were used to evaluate the success of the BSS rat model and RWCI efficacy. 14 and 33 differential metabolites were identified from plasma and urine samples using the metabolomics approach based on ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The results of multivariate analysis displayed that there were significant separations among model, control, and treatment groups, but the high-dose RWCI treatment group was closer to the control group. 9 perturbed metabolic pathways were related to BSS's development and RWCI intervention. 5 metabolic pathways (arachidonic acid metabolism, linoleic acid metabolism, alpha-linolenic acid metabolism, retinol metabolism, and steroid hormone biosynthesis) showed apparent correlations. These differential metabolites and perturbed metabolic pathways might provide a novel view to understand the pathogenesis of BSS and the pharmacological mechanism of RWCI.

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Section: Resultsmentioning

confidence: 99%

Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS

Ran

Pang

Guan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Similar to the PCA score map, ND and HFD were well separated from each other, whereas HFD+PRbs and HFD+Rbs partially overlapped and were more closely related to ND ( Figure 3B). After normalizing the obtained data with the Pareto scaling method [23,24], we analyzed the data with unsupervised principal component analysis (PCA), a multivariate statistical method which reduces the number of observations through dimensionality reduction into a few comprehensive indicators while it preserves the main information of the original variables ( Figure 3A). PC 1 and 2 explain 36.2% and 19.5% of the metabolic variance of serum, respectively.…”

Section: Analysis Of Serum Metabolitesmentioning

confidence: 99%

1H-NMR Metabolomics Analysis of the Effect of Rubusoside on Serum Metabolites of Golden Hamsters on a High-Fat Diet

Jiang

et al. 2020

Molecules

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Rubusoside is a natural sweetener and the active component of Rubus suavissimus. The preventive and therapeutic effect of rubusoside on high-fat diet-induced (HFD) serum metabolite changes in golden hamsters was analyzed by 1H-NMR metabolomics to explore the underlying mechanism of lipid metabolism regulation. 1H-NMR serum metabolomics analyses revealed a disturbed amino acid-, sugar-, fat-, and energy metabolism in HFD animals. Animals supplemented with rubusoside can partly reverse the metabolism disorders induced by high-fat diet and exerted good anti-hypertriglyceridemia effect by intervening in some major metabolic pathways, involving amino acid metabolism, synthesis of ketone bodies, as well as choline and 4-hydroxyphenylacetate metabolism. This study indicates that rubusoside can interfere with and normalize high-fat diet-induced metabolic changes in serum and could provide a theoretical basis to establish rubusoside as a potentially therapeutic tool able to revert or prevent lipid metabolism disorders.

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“…To evaluate the validity of the established OSC-PLS-DA model, 200 permutation tests and repeated 2-fold cross-validation (2CV) were performed 45,46 . The quality of the model was assessed based on R2 and Q2 scores, as R2 explained the total variation of the data, and Q2 described the predictive ability of the model 47 . Differential metabolites were assessed based on VIP values (VIP > 1) and t-tests (P < 0.05) 48 .…”

Section: Mh Sample Acquisitionmentioning

confidence: 99%

Metabolomics-based study reveals the effect of lead (Pb) in the culture environment on Whitmania pigra

Luo

Meng

Chen

et al. 2020

Sci Rep

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Whitmania pigra, called Mahuang (MH) in Chinese, has been used as a traditional Chinese medicine for many years and is susceptible to Pb exposure in aquaculture environments. To understand the impact of Pb in the culture environment on MHs, we carried out a 50-day culture of MHs in environments with different levels of Pb pollution. Then, tissue samples of MHs reared in the different Pb-polluted environments were collected and analysed by UPLC-Q/TOF-MS. The results showed that the Pb residue in MHs increased with increasing Pb in the culture environment. There was no significant difference in MH Pb content (P < 0.05) between the low-Pb residue group (PbL) and the blank control group (BC), and those of the middle-Pb residue group (PbM) and the high-Pb residue group (PbH) were significantly different from that of the BC group. Metabolomics results showed significant changes in 24 metabolites in the PbL, PbM and PbH groups, some of which were dose-dependent. These metabolites were mainly lipids, nucleotides, and dipeptides, which are involved in metabolic pathways such as glycerophospholipid metabolism, sphingolipid metabolism, and nucleotide metabolism. Overall, the results proved that metabolomics can be an effective tool to understand the effects of Pb on the metabolic responses of MHs.

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Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach

Cited by 32 publications

References 54 publications

Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS

Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS

1H-NMR Metabolomics Analysis of the Effect of Rubusoside on Serum Metabolites of Golden Hamsters on a High-Fat Diet

Metabolomics-based study reveals the effect of lead (Pb) in the culture environment on Whitmania pigra

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