Mechanism of biological denitrification inhibition: procyanidins induce an allosteric transition of the membrane-bound nitrate reductase through membrane alteration

Bardon, Clément; Poly, Franck; Piola, Florence; Pancton, Muriel; Comte, Gilles; Meiffren, Guillaume; Haichar, Feth el Zahar

doi:10.1093/femsec/fiw034

Cited by 53 publications

(14 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the PGPTs, the nitrogen cycle mediated by microbes has been shown to be important in alien plant invasion, such as Fallopia spp. in Europe (Bardon et al, 2014, 2016) and Sorghum halepense in North America (Rout & Chrzanowski, 2009; Rout et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Diversity analysis of the rhizospheric and endophytic bacterial communities of Senecio vulgaris L. (Asteraceae) in an invasive range

Cheng

Tian

Feng

et al. 2019

PeerJ

View full text Add to dashboard Cite

Increasing evidence has confirmed the importance of plant-associated bacteria for plant growth and productivity, and thus it is hypothesized that interactions between bacteria and alien plants might play an important role in plant invasions. However, the diversity of the bacterial communities associated with invasive plants is poorly understood. We therefore investigated the diversity of rhizospheric and endophytic bacteria associated with the invasive annual plant Senecio vulgaris L. (Asteraceae) based on 16S rRNA gene data obtained from 57 samples of four Senecio vulgaris populations in a subtropical mountainous area in central China. Significant differences in diversity were observed between plant compartments. Specifically, the rhizosphere harbored many more bacterial operational taxonomic units and showed higher alpha diversity than the leaf and root endospheres. The relative abundance profiles of the bacterial community composition differed substantially between the compartments and populations, especially at the phylum and family levels. However, the top five phyla (Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Acidobacteria) accounted for more than 90% of all the bacterial communities. Moreover, similar endophytic communities with a shared core set of bacteria were observed from different Senecio vulgaris populations. Heavy-metal-resistant, phosphate-solubilizing bacteria (Brevundimonas diminuta), nitrogen-fixing bacteria (Rhizobium leguminosarum), and cold-resistant bacteria (Exiguobacterium sibiricum) were present in the endosphere at relatively high abundance. This study, which reveals the structure of bacterial communities and their putative function in invasive Senecio vulgaris plants, is the first step in investigating the role of plant–bacteria interactions in the invasion of this species in China.

show abstract

Section: Discussionmentioning

confidence: 99%

Diversity analysis of the rhizospheric and endophytic bacterial communities of Senecio vulgaris L. (Asteraceae) in an invasive range

Cheng

Tian

Feng

et al. 2019

PeerJ

View full text Add to dashboard Cite

show abstract

“…This implication is important because bifunctional herbicides that can effectively prevent the loss of nitrogen by denitrification as well as control the growth of weed can possibly be developed with a molecular design and bio-rational screening systems [9,10,34]. Thus far to our knowledge, the mechanism and mode of action of biological denitrification inhibition are rarely known, but some reliable reports have been published [35,36].…”

Section: Discussionmentioning

confidence: 99%

Effects of Some Hill Reaction-Inhibiting Herbicides on Nitrous Oxide Emission from Nitrogen-Input Farming Soil

Takatsu

Lau

et al. 2019

Applied Sciences

View full text Add to dashboard Cite

Nitrous oxide (N2O) emission-suppressing activity of some electron-transport inhibitors of the Hill reaction system was investigated. The Hill reaction inhibitors—paraquat, isouron, bromacil, diquat, and simazine—all of which have been or are currently being used as herbicides in farming activity are expected to inhibit the electron-transporting pathways of nitrate respiration in denitrifying bacteria. Using N2O-emitting soil bed (5.0 g of fresh weight) from a continuously manured Andisol corn farmland in Hokkaido, Japan, which was autoclaved and further supplemented with an active N2O-emitter, Pseudomonas sp. 5CFM15-6D, and 1 mL of 100 mM NH4NO3 or (NH4)2SO4 solution as the sole nitrogen source (final concentration, 0.2 mM) in a 30 mL gas-chromatography vial, the effects of the five herbicides on N2O emission were examined. Paraquat and isouron (each at 50 µM) showed a statistically significant suppression of N2O emission in both the nitrification and the denitrification processes after a 7-day-incubation, whereas diquat at the same concentration accelerated N2O emission in the presence of NO3−. These results suggest that paraquat and isouron inhibited both the nitrification and the denitrification processes for N2O generation, or its upstream stages, whereas diquat specifically inhibited N2O reductase, an enzyme that catalyzes the reduction of N2O to N2 gas. Incomplete denitrifiers are the key players in the potent emission of N2O from Andisol corn farmland soil because of the missing nosZ gene. The electron relay system-inhibiting herbicides—paraquat and isouron—possibly contribute to the prevention of denitrification-induced nitrogen loss from the farming soil.

show abstract

“…(Asian knotweed). This compound has been demonstrated to be an allosteric inhibitor of Pseudomonas brassicacearum nitrate reductase and while it does reduce denitrification in the soil, it has not yet been proven to mitigate N 2 O levels [57]; (6) Microbial bioremediation [88]. The success of N fertilizer management techniques and proper irrigation is largely due to the creation of a microsphere conducive to denitrifying bacteria flourishing [89].…”

Section: Mechanism Of Action Pros Consmentioning

confidence: 99%

New Breeding Techniques for Greenhouse Gas (GHG) Mitigation: Plants May Express Nitrous Oxide Reductase

Demone

Wan

Nourimand

et al. 2018

Climate

View full text Add to dashboard Cite

Nitrous oxide (N2O) is a potent greenhouse gas (GHG). Although it comprises only 0.03% of total GHGs produced, N2O makes a marked contribution to global warming. Much of the N2O in the atmosphere issues from incomplete bacterial denitrification processes acting on high levels of nitrogen (N) in the soil due to fertilizer usage. Using less fertilizer is the obvious solution for denitrification mitigation, but there is a significant drawback (especially where not enough N is available for the crop via N deposition, irrigation water, mineral soil N, or mineralization of organic matter): some crops require high-N fertilizer to produce the yields necessary to help feed the world’s increasing population. Alternatives for denitrification have considerable caveats. The long-standing promise of genetic modification for N fixation may be expanded now to enhance dissimilatory denitrification via genetic engineering. Biotechnology may solve what is thought to be a pivotal environmental challenge of the 21st century, reducing GHGs. Current approaches towards N2O mitigation are examined here, revealing an innovative solution for producing staple crops that can ‘crack’ N2O. The transfer of the bacterial nitrous oxide reductase gene (nosZ) into plants may herald the development of plants that express the nitrous oxide reductase enzyme (N2OR). This tactic would parallel the precedents of using the molecular toolkit innately offered by the soil microflora to reduce the environmental footprint of agriculture.

show abstract

Mechanism of biological denitrification inhibition: procyanidins induce an allosteric transition of the membrane-bound nitrate reductase through membrane alteration

Cited by 53 publications

References 51 publications

Diversity analysis of the rhizospheric and endophytic bacterial communities of Senecio vulgaris L. (Asteraceae) in an invasive range

Diversity analysis of the rhizospheric and endophytic bacterial communities of Senecio vulgaris L. (Asteraceae) in an invasive range

Effects of Some Hill Reaction-Inhibiting Herbicides on Nitrous Oxide Emission from Nitrogen-Input Farming Soil

New Breeding Techniques for Greenhouse Gas (GHG) Mitigation: Plants May Express Nitrous Oxide Reductase

Contact Info

Product

Resources

About