Mechanism of Arsenate Resistance in the Ericoid Mycorrhizal Fungus <i>Hymenoscyphus ericae</i>

Sharples, J. M.; Meharg, Andrew A.; Chambers, Susan M.; Cairney, John

doi:10.1104/pp.124.3.1327

Cited by 128 publications

(79 citation statements)

References 43 publications

(29 reference statements)

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“…From the present study it is not clear whether AMF function like ericoid mycorrhizal fungi that can protect their host plant against As contamination by reducing arsenate to arsenite and pumping out the latter (Sharples et al, 1999(Sharples et al, , 2000a. Knudson et al (2003) reported that AMF neither conferred As tolerance in basin wild-rye (Leymus cinereus [Scribner and Merr.]…”

Section: Discussionmentioning

confidence: 83%

The arbuscular mycorrhizal fungus Glomus mosseae gives contradictory effects on phosphorus and arsenic acquisition by Medicago sativa Linn

Chen

Xiao

Zhu

et al. 2007

Science of The Total Environment

141

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Section: Discussionmentioning

confidence: 83%

The arbuscular mycorrhizal fungus Glomus mosseae gives contradictory effects on phosphorus and arsenic acquisition by Medicago sativa Linn

Chen

Xiao

Zhu

et al. 2007

Science of The Total Environment

141

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“…This has resulted in increased P/As ratios and may enhance arsenic resistance of the brake fern. Sharples et al (2000) reported that Calluna vulgaris formed an ericoid mycorrhizal association and the mycorrhizal fungus, Hymenoscyphus ericae, played a major role in plant resistance to As by selectively accumulating phosphate over arsenate. The work of Gonzalez-Chavez et al (2002) demonstrated that AMF on mine spoils have evolved arsenate resistance, and conferred enhanced resistance on Holcus lanatus.…”

Section: Discussionmentioning

confidence: 99%

“…P. vittata forms arbuscular mycorrhizas (Fitz and Wenzel, 2002), but the role of AMF in phytoremediation of As-contaminated soil using this fern plant has received only little attention. Isolates of AMF from an As/ Cu mine site were shown to be able to maintain a relatively low As concentration in the host plant via efflux (Sharples et al, 2000). However, the enlarged plant biomass due to improved mineral nutrition may still lend advantages to mycorrhizal plants for remediation of As contaminated soils (Liu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Effects of arbuscular mycorrhizal inoculation on uranium and arsenic accumulation by Chinese brake fern (Pteris vittata L.) from a uranium mining-impacted soil

2006

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A glasshouse experiment was conducted to investigate U and As accumulation by Chinese brake fern, Pteris vittata L., in association with different arbuscular mycorrhizal fungi (AMF) from a U and As contaminated soil. The soil used contains 111 mg U kg À1 and 106 mg As kg À1. P. vittata L. was inoculated with each of three AMF, Glomus mosseae, Glomus caledonium and Glomus intraradices. Two harvests were made during plant growth (two and three months after transplanting). Mycorrhizal colonization depressed plant growth particularly at the early stages. TF (transfer factor) values for As from soil to fronds were higher than 1.0, while those for roots were much lower. Despite the growth depressions, AM colonization had no effect on tissue As concentrations. Conversely, TF values for U were much higher for roots than for fronds, indicating that only very small fraction of U was translocated to fronds (less than 2%), regardless of mycorrhizal colonization. Mycorrhizal colonization significantly increased root U concentrations at both harvests. Root colonization with G. mosseae or G. intraradices led to an increase in TF values for U from 7 (non-inoculation control) to 14 at the first harvest. The highest U concentration of 1574 mg kg À1 was recorded in roots colonized by G. mosseae at the second harvest. The results suggested that P. vittata in combination with appropriate AMF would play very important roles in bioremediation of contaminated environments characterized by a multi-pollution.

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“…Arsenate is efficiently reduced into arsenite (AsO 3 23 ) by GSH-dependent arsenate reductase activities in bacteria, fungi, plants, and animals (Oden et al, 1994;Mukhopadhyay et al, 1998;Dhankher et al, 2002Dhankher et al, , 2006Dong et al, 2005). Arsenite is highly thiolreactive and may be chelated and sequestrated as As(S-R) 3 thiol-peptide complexes during its detoxification (Dey et al, 1994;Rosen, 1999;Sharples et al, 2000). Thus, the significant levels of arsenic tolerance observed for the S1ptTECS/cad2-1 transgenic CS1 plants examined in this study must be at least in part due to high levels of EC and its downstream products in both roots and shoots relative to wild-type plants.…”

Section: Element Accumulation In Shootsmentioning

confidence: 99%

The Shoot-Specific Expression of γ-Glutamylcysteine Synthetase Directs the Long-Distance Transport of Thiol-Peptides to Roots Conferring Tolerance to Mercury and Arsenic

et al. 2006

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Thiol-peptides synthesized as intermediates in phytochelatin (PC) biosynthesis confer cellular tolerance to toxic elements like arsenic, mercury, and cadmium, but little is known about their long-distance transport between plant organs. A modified bacterial γ-glutamylcysteine synthetase (ECS) gene, S1pt∷ECS, was expressed in the shoots of the ECS-deficient, heavy-metal-sensitive cad2-1 mutant of Arabidopsis (Arabidopsis thaliana). S1pt∷ECS directed strong ECS protein expression in the shoots, but no ECS was detected in the roots of transgenic plant lines. The S1pt∷ECS gene restored full mercury tolerance and partial cadmium tolerance to the mutant and enhanced arsenate tolerance significantly beyond wild-type levels. After arsenic treatment, the root concentrations of γ-glutamylcysteine (EC), PC2, and PC3 peptides in a S1pt∷ECS-complemented cad2-1 line increased 6- to 100-fold over the mutant levels and were equivalent to wild-type concentrations. The shoot and root levels of glutathione were 2- to 5-fold above those in wild-type plants, with or without treatment with toxicants. Thus, EC and perhaps glutathione are efficiently transported from shoots to roots. The possibility that EC or other PC pathway intermediates may act as carriers for the long-distance phloem transport and subsequent redistribution of thiol-reactive toxins and nutrients in plants is discussed.

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Mechanism of Arsenate Resistance in the Ericoid Mycorrhizal Fungus Hymenoscyphus ericae

Abstract: Arsenate resistance is exhibited by the ericoid mycorrhizal fungus Hymenoscyphus ericae collected from As-contaminated mine soils. To investigate the mechanism of arsenate resistance, uptake kinetics for arsenate (H 2

Cited by 128 publications

References 43 publications

The arbuscular mycorrhizal fungus Glomus mosseae gives contradictory effects on phosphorus and arsenic acquisition by Medicago sativa Linn

The arbuscular mycorrhizal fungus Glomus mosseae gives contradictory effects on phosphorus and arsenic acquisition by Medicago sativa Linn

Effects of arbuscular mycorrhizal inoculation on uranium and arsenic accumulation by Chinese brake fern (Pteris vittata L.) from a uranium mining-impacted soil

The Shoot-Specific Expression of γ-Glutamylcysteine Synthetase Directs the Long-Distance Transport of Thiol-Peptides to Roots Conferring Tolerance to Mercury and Arsenic

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