Mechanism of antifungal activity of Perilla frutescens essential oil against Aspergillus flavus by transcriptomic analysis

Hu, Zhenyang; Kang, Yuan; Zhou, Qi Tony; Chen, Lu; Du, Lei; Liu, Fang

doi:10.1016/j.foodcont.2020.107703

Cited by 93 publications

(30 citation statements)

References 50 publications

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“…The above results indicated that P. digitatum is responsible for maintaining the fluidity and permeability of the cell membrane by adjusting the composition and content of fatty acids. A similar antifungal mechanism was also reported by the study described by Hu et al ( 39 ). Membrane is the target of Perilla frutescens essential oil against A. flavus .…”

Section: Discussionsupporting

confidence: 87%

“…Under the influence of external stimuli, P. digitatum cells alleviate the adverse effects on growth by regulating gene expression patterns. Several studies have proved that the antimicrobial mechanism of essential oil includes alternating the distribution of fatty acids in the cell membrane, destroying cell walls, and reducing proton power, and the inactivation of ATPase, amylase, and protease was promoted (35)(36)(37)(38)(39)(40). To deeply understand the action mechanism of nanoemulsion from molecular aspects and seek the pathway involved, we used RNA-Seq technology to profile the transcriptome of P. digitatum treated with nanoemulsion, which induced the expression of many stress response genes and the pathway involved to alleviate the adverse effect.…”

Section: Discussionmentioning

confidence: 99%

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Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

et al. 2021

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Penicillium digitatum is the most severe pathogen that infects citrus fruits during storage. It can cause fruit rot and bring significant economic losses. The continuous use of fungicides has resulted in the emergence of drug-resistant strains. Consequently, there is a need to develop naturally and efficiently antifungal fungicides. Natural antimicrobial agents such as clove oil, cinnamon oil, and thyme oil can be extracted from different plant parts. They exhibited broad-spectrum antimicrobial properties and have great potential in the food industry. Here, we exploit a novel cinnamaldehyde (CA), eugenol (EUG), or carvacrol (CAR) combination antifungal therapy and formulate it into nanoemulsion form to overcome lower solubility and instability of essential oil. In this study, the antifungal activity evaluation and transcriptional profile of Penicillium digitatum exposed to compound nanoemulsion were evaluated. Results showed that compound nanoemulsion had a striking inhibitory effect on P. digitatum in a dose-dependent manner. According to RNA-seq analysis, there were 2,169 differentially expressed genes (DEGs) between control and nanoemulsion-treated samples, including 1,028 downregulated and 1,141 upregulated genes. Gene Ontology (GO) analysis indicated that the DEGs were mainly involved in intracellular organelle parts of cell component: cellular respiration, proton transmembrane transport of biological process, and guanyl nucleotide-binding molecular function. KEGG analysis revealed that metabolic pathway, biosynthesis of secondary metabolites, and glyoxylate and dicarboxylate metabolism were the most highly enriched pathways for these DEGs. Taken together, we can conclude the promising antifungal activity of nanoemulsion with multiple action sites against P. digitatum. These outcomes would deepen our knowledge of the inhibitory mechanism from molecular aspects and exploit naturally, efficiently, and harmlessly antifungal agents in the citrus postharvest industry.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

et al. 2021

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“…Upregulation of DEGs was observed after longterm fumigation of P. expansum with decanal that further leads to generating cellular stress molecules culminating in cell death. Hu et al (2021) demonstrated the antifungal activity of Perilla fruitscens EO against A. flavus by transcriptome analysis of DEGs involving amino acid metabolism, membrane permeability, oxidative stress, and spore development. A total of 5,914 DEGs were investigated; among them, 3,025 genes were upregulated, whereas 2,889 genes were downregulated with respect to EO treatment.…”

Section: Effect On Dna and Gene Expressionmentioning

confidence: 99%

Essential Oils and Their Application in Food Safety

Maurya

Prasad

Das

et al. 2021

Front. Sustain. Food Syst.

161

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Food industries are facing a great challenge due to contamination of food products with different microbes such as bacteria, fungi, viruses, parasites, etc. These microbes deteriorate food items by producing different toxins during pre- and postharvest processing. Mycotoxins are one of the most potent and well-studied toxic food contaminants of fungal origin, causing a severe health hazard to humans. The application of synthetic chemicals as food preservatives poses a real scourge in the present scenario due to their bio-incompatibility, non-biodegradability, and environmental non-sustainability. Therefore, plant-based antimicrobials, including essential oils, have developed cumulative interest as a potential alternative to synthetic preservatives because of their ecofriendly nature and generally recognized as safe status. However, the practical utilization of essential oils as an efficient antimicrobial in the food industry is challenging due to their volatile nature, less solubility, and high instability. The recent application of different delivery strategies viz. nanoencapsulation, active packaging, and polymer-based coating effectively addressed these challenges and improved the bioefficacy and controlled release of essential oils. This article provides an overview of essential oils for the preservation of stored foods against bacteria, fungi, and mycotoxins, along with the specialized mechanism of action and technological advancement by using different delivery systems for their effective application in food and agricultural industries smart green preservative.

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“…Although previous studies also reported plant-derived natural compounds in damaging cell wall integrity in conditional pathogens, the detailed mechanisms are not consistent. Hu et al reported that Perilla frutescens essential oil downregulated α-1,3-glucan synthase AGS1 gene and four chitinase genes in A. flavus [ 38 ]. Cinnamaldehyde was capable to target β-1,3-glucan synthase and chitin synthase in Saccharomyces.…”

Section: Resultsmentioning

confidence: 99%

Paeonol Disrupts the Integrity of Aspergillus flavus Cell Walls via Releasing Surface Proteins, Inhibiting the Biosynthesis of β-1,3-Glucan and Promoting the Degradation of Chitin, and an Identification of Cell Surface Proteins

Zhao

Xie

2021

Foods

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Paeonol can effectively inhibit Aspergillus flavus (A. flavus) via damaging cell walls. In this work, paeonol treatment remarkably destroyed both the outer amorphous layer and the inner fibrous layer of cell walls. Furthermore, FT-IR and XPS characterization showed that OH functional groups were altered and proteins in the outer layer were released. According to proteomic analysis, 605 proteins have been identified and annotated. The activities of β-1,3-glucan synthase and chitinase were prohibited and promoted, respectively, by paeonol treatment, however, the activities of β-1,3-glucanase and chitin synthase were not influenced. QRT-PCR results suggested that FKSP, CHIIII, and CHIV genes might be the antifungal targets of paeonol. In addition, paeonol can effectively restrain the pathogenicity of A. flavus on peanut butter. This study provided a new elucidation on the mode of action of paeonol against cell walls of A. flavus, facilitating the application of paeonol in the preservation of agricultural products.

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Mechanism of antifungal activity of Perilla frutescens essential oil against Aspergillus flavus by transcriptomic analysis

Cited by 93 publications

References 50 publications

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

Essential Oils and Their Application in Food Safety

Paeonol Disrupts the Integrity of Aspergillus flavus Cell Walls via Releasing Surface Proteins, Inhibiting the Biosynthesis of β-1,3-Glucan and Promoting the Degradation of Chitin, and an Identification of Cell Surface Proteins

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