Mechanism of Aniline Degradation by Yeast Strain Candida methanosorbosa BP-6

Abstract

The ability of some bacteria and filamentous fungi to degrade aniline and its derivatives was reported earlier in the literature. However, there was no information about the biodegradation of aniline by yeast strains. The present work is focused on yeast strain Candida methanosorbosa BP-6 which was isolated from the wastewater pool of the old dye factory "Boruta" in Zgierz by enrichment technique and identified by standard microbiological methods. We have found that strain C. methanosorbosa BP-6 readily grows in the presence of aniline and can degrade this substrate. Relatively good separation of peaks corresponding to aniline and its biodegradation intermediates allowed us their identification and quantification by HPLC methodology. We have found that major intermediates of this degradation are: catechol, cis,cis-muconic acid, muconolactone, 3-oxoadipate enol-lactone, 3-oxoadipic acid and succinic acid. Our results provide strong evidence that biodegradation of aniline by the yeast strain C. methanosorbosa BP-6 proceeds according to the intradiolic pathway.

Biodegradation: Involved Microorganisms and Genetically Engineered Microorganisms

“…Rhodotorula aurantiaca and C. ernobii were found able to degrade diesel oil [66]. Yeasts are also reported for aniline biodegradation (a potential degradation product of the azo dye breakdown) it is the example of C. methanosorbosa BP-6 [67]. According to many authors, bacteria have been described as being more efficient hydrocarbon degraders than yeast, or at least that bacteria are more commonly used as a test microorganism.…”

Wheat phyllosphere yeasts degrade propiconazole

“…According to Chandran and Das [15], yeast species C. tropicalis, Cryptococcus laurentii, Trichosporon asahii, Rhodotorula mucilaginosa and Candida rugosa with active cytochrome P450 enzymes participate in the degradation of diesel oil in soil. Selected strains of Candidia methanosorbosa degraded anilines, the putative degradation products of azo dyes [16]. Some species of yeasts and bacteria can transform organic aromatic pollutants, including polycyclic aromatic hydrocarbons, biphenyls, dibenzofurans and pesticides, through cometabolism [17].…”

An Overview of Aniline and Chloroaniline Compounds as Environmental Pollutants

“…Due to their wide-ranging of toxic impact and carcinogenicity, wastewater containing aniline compound is classified as hazardous waste [10]. The main toxic effects of aniline start with the creation of methemoglobin in blood are expressed by dropping of oxygen carrying, anaemia, jaundice, damage of liver, spleen plus lungs and reduced blood coagulation [18,19]. Chloroanilines are toxic compounds which hinder oxidative phosphorylation and microbial growth.…”