Mechanism of action of the stimulatory effect of apigenin-6-C-(2″-O-α-l-rhamnopyranosyl)-β-l-fucopyranoside on 14C-glucose uptake

Identification of trans-tiliroside as active principle with anti-hyperglycemic, anti-hyperlipidemic and antioxidant effects from Potentilla chinesis

“…The beneficial effects of several flavonol glycosides have been demonstrated in relation to diabetes mellitus, through acting at multiple sites of glucose regulatory pathways, e.g. glucose tolerance, lipid profile, glycogen biosynthesis, glucose uptake and insulin release (Sharma et al, 2008;Cazarolli et al, 2009). Although many bioactivities of the principal constituent, transtiliroside were reported, such as hepatoprotective inhibition of the production of nitric oxide and tumor necrosis factor-␣ in macrophages, inhibition of human low density lipoprotein oxidation (Schinella et al, 2007), anti-obese (Ninomiya et al, 2007), anti-inflammatory (Sala et al, 2003) and anticomplement activities .…”

Triterpene derivative: A potential signaling pathway for the fern-9(11)-ene-2α,3β-diol on insulin secretion in pancreatic islet

“…Insulin was estimated by means of colorimetric measurements at 450 nm with an ELISA plate reader (Organon Teknika, Roseland, NJ, USA) by interpolation from a standard curve. Samples were analyzed in duplicate and results were expressed as nanograms of insulin serum per milliliter [22].…”

“…After incubation, the islet samples were homogenized in 0.5 N NaOH and boiled for 10 min; 25 μL aliquots of tissue and external medium were placed in scintillation liquid on an LKB RackBeta liquid scintillation spectrometer (model 1215; EG and G-Wallac, Turku, Finland), for the radioactivity measurements. Samples were processed as described by Cazarolli et al [22] and, for total protein quantification, the method of Lowry [25] was used. The glucose uptake results were expressed as nmol glucose units/mg of protein.…”

Antihyperglycemic activity of naphthylchalcones

“…All insulin levels were estimated by means of colorimetric measurements at 450 nm with an ELISA plate reader (Organon Teknika, Roseland, NJ, USA) by interpolation from a standard curve. Samples were analyzed in duplicate and results were expressed as ng of insulin serum mL À1 [33].…”