Mechanism of action of mouse macrophages as antitumor effector cells: Role of arginase

Farram, E; Nelson, D. S.

doi:10.1016/0008-8749(80)90161-6

Cited by 29 publications

(4 citation statements)

References 22 publications

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“…The change of Acp activity may reflect changes in the cytoplasmic ionized calcium and/or cyclic adenosine 5′‐monophosphate (AMP) level 41. Nonspecific antitumor cytotoxicity of macrophages involves the release of Arg 42. The increase of the activity of these two lysosomal enzymatic markers gives an indication that C 60 (OH) x treatment stimulates significantly the activation of macrophages and thus could be responsible for the inhibition of tumor growth.…”

Section: Resultsmentioning

confidence: 99%

Tumor‐Inhibitory Effect and Immunomodulatory Activity of Fullerol C₆₀(OH)_x

Zhu

Wang

et al. 2008

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The tumor-inhibitory effect of C60(OH)x was tested on the murine H22 hepatocarcinoma model. Doses of 0.2 and 1.0 mg kg(-1) body weight both showed significant antitumor activity with tumor inhibition rates of 31.9 and 38.4%, respectively, when mice were treated for 17 consecutive days. The damnification of liver was prominently reduced. Furthermore, histological examination indicated that an envelope of fibroblasts and lymphocytes was formed surrounding tumor tissues in the C60(OH)x-treated group, which inhibited the infiltration of tumor to the neighboring normal skeleton muscle tissues. To understand the antitumor mechanism, the immunomodulatory activity of C60(OH)x was investigated. The results indicate that C60(OH)x enhances the phagocytosis of peritoneal macrophages and elevates the activity of arginase and acid phosphatase in vivo. The tumor necrosis factor alpha production of C60(OH)x-treated macrophages also increases in vitro. These results suggest that C60(OH)x can enhance the innate immunity of tumor-bearing mice, and therefore inhibits growth of the tumor.

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Section: Resultsmentioning

confidence: 99%

Tumor‐Inhibitory Effect and Immunomodulatory Activity of Fullerol C₆₀(OH)_x

Zhu

Wang

et al. 2008

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“…The origin, passage, storage and maintenance of the following tumours and cells has been described elsewhere: SCS-3, a spontaneously arising spindle cell sarcoma of CBA/J mice; A-2, a methylcholanthrene-induced sarcoma of A/J mice; EL-4, a lymphoma of C57B1 mice; D7, a methylcholanthrene-induced sarcoma of DA rats; D8M, a metastatic variant of another methylcholanthrene-induced sarcoma of DA rats; human liver Chang cells; MM200, a human malignant melanoma; HeLa cells, originally from a human cervical carcinoma; mouse fibroblasts Nelson, 1978, 1980;Farram and Nelson, 1980;Farram et al, 1981Farram et al, , 1982Inoue and Nelson, 1982). The tumours appear to be free of lactic dehydrogenase virus, infection with which can alter host immune responsiveness (Notkins, 1971).…”

Section: Tumours and Other Cellsmentioning

confidence: 99%

Effects of Tumour Cell Culture Supernatants on Some Biochemical Activities of Macrophages

Nelson

Booth

Nelson

1982

Aust J Exp Biol Med

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Summary The following enzyme activities were measured in cell lysates and supernatants from mouse peritoneal macrophages incubated with products of cultured tumour and other cells: acid phosphatase, β‐D‐glucuronidase, N‐acetyl‐D‐glucosaminidase, muramidase (lysozyme), lactic dehydrogenase (supernatant only) and plasminogen activator (supernatant only). There were no noteworthy changes in enzyme activities. Hydrogen peroxide production by appropriately stimulated mouse and/or guinea‐pig peritoneal exudate macrophages was variably inhibited by supernatants from some tumour cells and some normal cells. Changes in these biochemical activities of macrophages do not appear to be closely related to the anti‐inflammatory activity of tumour cell products.

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“…motility of monocytes separated on a Percoll gradient, as compared with cells separated on a Ficoll/Hypaque gradient, and Serio et al (1979) observed a 6% inhibition of phagocytic activity after incubation of mouse peritoneal macrophages in an 8% (v/v) suspension of Percoll. Others have reported that monocyte/macrophage activities are unimpaired after isolation in Percoll gradients, including Trypan Blue exclusion and ability of monocytes to transform into macrophages (Fluks, 1981), phagocytosis (Gmelig-Meyling & Waldmann, 1980), cytotoxic ability (Farram & Nelson, 1980) and Fc receptor activity (Pertoft et al, 1980). Fc receptors, phagocytic activity and non-specific esterase activity were found to be present in cultures of Kupffer cells previously isolated by centrifugation in a density gradient of Percoll (Pulford & Souhami, 1980).…”

Section: Resultsmentioning

confidence: 99%

Is Percoll innocuous to cells?

Wakefield¹,

Gale²,

Berridge³

et al. 1982

Biochemical Journal

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Peritoneal macrophages from mice, isolated rat liver Kupffer cells and rat testis Leydig cells ingested large numbers of Percoll particles, a gradient medium widely used for separation of cells and subcellular organelles by density-gradient centrifugation. A decrease in the percentage of macrophages adhering to plastic also occurred after exposure of the cells to Percoll, even at 4 degrees C, a temperature at which Percoll was not ingested. The effect of Percoll on macrophage adherence may involve a loose association between the density medium and the cell surface. Other cell-surface-related phenomena may also be affected by prior exposure of cells to Percoll.

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Mechanism of action of mouse macrophages as antitumor effector cells: Role of arginase

Cited by 29 publications

References 22 publications

Tumor‐Inhibitory Effect and Immunomodulatory Activity of Fullerol C₆₀(OH)_x

Tumor‐Inhibitory Effect and Immunomodulatory Activity of Fullerol C₆₀(OH)_x

Effects of Tumour Cell Culture Supernatants on Some Biochemical Activities of Macrophages

Is Percoll innocuous to cells?

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Mechanism of action of mouse macrophages as antitumor effector cells: Role of arginase

Cited by 29 publications

References 22 publications

Tumor‐Inhibitory Effect and Immunomodulatory Activity of Fullerol C60(OH)x

Tumor‐Inhibitory Effect and Immunomodulatory Activity of Fullerol C60(OH)x

Effects of Tumour Cell Culture Supernatants on Some Biochemical Activities of Macrophages

Is Percoll innocuous to cells?

Contact Info

Product

Resources

About

Tumor‐Inhibitory Effect and Immunomodulatory Activity of Fullerol C₆₀(OH)_x

Tumor‐Inhibitory Effect and Immunomodulatory Activity of Fullerol C₆₀(OH)_x