Mechanism of Action of L-arginine on the Vitality of Spermatozoa is Primarily Through Increased Biosynthesis of Nitric Oxide

Srivastava, Sudha; Desai, Prashant; Coutinho, Evans C.; Govil, Girjesh

doi:10.1095/biolreprod.105.046896

Cited by 68 publications

(53 citation statements)

References 13 publications

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“…1). Isto pode ser explicado pelo fato de a via NOS/NO possuir importante ação antioxidante, que protege a célula da peroxidação lipídica (Srivastava et al, 2006;Leal et al, 2009), que resultaria na modificação de moléculas lipídicas da membrana plasmática do espermatozoide, diminuindo sua viabilidade (Thomas et al, 2008). Este achado confirma os resultados obtidos por Hellstrom et al (1994), que observaram que a adição de 50 a 100nM de SNP ao sêmen humano criopreservado levou à diminuição da peroxidação lipídica e ao aumento da integridade da membrana plasmática do espermatozoide com efeito dose-dependente durante 6h de avaliação.…”

Section: Resultsunclassified

Efeito da inibição da óxido nítrico sintase induzível na capacitação in vitro de espermatozoides bovinos

Ferreira-Berbari¹,

Caldas-Bussiere²,

Carvalho³

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliaram-se o papel do óxido nítrico (NO) por meio da inibição da enzima óxido nítrico sintase induzível (iNOS), após a adição da aminoguanidina (AG), na motilidade, no vigor e na integridade da membrana plasmática nos tempos de 15, 60, 120, 180, 240 e 300min e a atividade mitocondrial e a capacitação de espermatozoides bovinos após 300min de cultivo. Adicionaram-se diferentes concentrações (0,001, 0,01 e 0,1M) de AG durante a capacitação induzida pela heparina e 500M de nitroprussiato de sódio (SNP, doador de NO) à concentração deletéria. A adição de 0,1M de AG diminuiu a motilidade e o vigor espermático e a integridade da membrana (P<0,05). A adição de SNP ao meio de cultivo com 0,1M de AG somente reverteu a integridade da membrana após 300min. A inibição da síntese de NO pela adição de AG não alterou a atividade mitocondrial. A percentagem de oócitos penetrados com espermatozoides tratados com 0,01 e 0,1M de AG diminuiu 20,3 e 100%, respectivamente, em relação aos não tratados (controle) (P<0,05), contudo houve aumento de 15% na percentagem de oócitos desnudados penetrados com espermatozoides capacitados em presença de 0,1M de AG. Conclui-se que a inibição da síntese de NO pela AG diminuiu a qualidade espermática durante a capacitação de espermatozoides bovinos in vitro, exceto a atividade mitocondrial. Somente a integridade da membrana foi revertida após adição de NO, sugerindo diferentes vias de ação do NO na qualidade espermática ao longo da capacitação in vitro de espermatozoides bovinos.Palavras-chave: bovino, óxido nítrico, qualidade do espermatozoide, capacitação in vitro, aminoguanidina 15, 60, 120, 180, 240, and 300min and on mitochondrial activity and capacitation after 300min, respectively. Different concentrations, 0.001, 0.01, and 0.1M of AG were added during the heparin induced capacitation and sodium nitroprusside (SNP, 01 and 0.1M of AG diminished, 20.3 and 100%, respectively, in ABSTRACT The role of nitric oxide (NO) was evaluated by inhibition of inducible nitric oxide synthase (iNOS), with aminoguanidine (AG) on motility, vigor, and plasmatic membrane integrity of bovine spermatozoa culture after

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Section: Resultsunclassified

Efeito da inibição da óxido nítrico sintase induzível na capacitação in vitro de espermatozoides bovinos

Ferreira-Berbari¹,

Caldas-Bussiere²,

Carvalho³

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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“…In addition, because the dose of L-Arginine given in the medium of capacitance is to balance the occurrence of lipid peroxidation or free radicals. L-Arginine is an amino acid that can stimulate the motility of mammalian spermatozoa under in vitro conditions and play an important role in the defense of cellular immunity 19 . L -Arginin protects spermatozoa against lipid peroxidation by increasing the production of nitric oxide and deactivating free radicals.…”

Section: Discussionmentioning

confidence: 99%

The Addition of L-Arginine in Capacitation Media to Motility, Viability, and Spermatozoa Capacity of Goats

2018

IJCTR

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:The purpose of this study is improving the provision of animal origin food of in vitro fertilization technology. Specific targets to be achieved in this study is to increase the incidence of capacitation spermatozoan and success in in vitro fertilization process . This research utilized four treatment groups, namely controlled group (P0) is the addition of LArginine in spermatozoa (HEPES) capacitance medium , group (PI) is the addition of LArginine in spermatozoa (HEPES) capacitation medium by 0,002 M / ml ,group (PII) is the addition of L-Arginine in spermatozoa (HEPES) capacitation medium by 0,004 M / ml. and group (PIII) is the addition of L-Arginine in spermatozoa (HEPES) capacitation medium by 0,006 M / ml Further evaluations included motility, viability, capacitation reaction of spermatozoa. Data obtained in the form of percentage of motility, viability and capacitation reaction is analyzed by F Test. The conclusion of this study, group (PIII) is the addition of LArginine in spermatozoa (HEPES) capacitation medium by 0,006 M / ml increase of motility, viability and capacitation reaction.

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“…L-Arginin is amino acid which can stimulated sperm motility in the in vitro condition.and also have role in celluler imunity defens [8]. L-Arginin such protect spermatozoa against lipid peroxidation through increased production of nitric oxide used againt reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and anion superoxide (O 2 ) [9].…”

Section: Discussionmentioning

confidence: 99%

Adding of L-Arginin Amino Acidin Skim Milk Diluent to Maintain Quality of Buck Sperm in Cold Temperature

Suprayogy

Susilowati

Hernawati

2017

KLS

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Nowdays, the storage of buck semen in cold temperature have not satisfied yet because in buck's seminal plasma contains phospholipase enzyme which can coagulated egg yolk in diluents.The specific aim of this study was to investigate the benefits of L-Arginin amino acid in skim diluents to quality buck's spermatozoa on cold temperature. This researchutilized four treatment groups, namely Controlled group (P0): skim milk diluent without L-Arginin + buck's semen; P1: skim milk diluents + L-Arginin 0,002M/ml + buck" semen; P2: skim milk diluents + L-Arginin 0,004 M/ml + buck's semen and P3: skim milk diluents + L-Arginin 0,006 M/ml + buck's semen. Then the samples stored in cold temperature (5 ∘ C). The result showed that sperm motility, viability and membrane integrity were significantly different (p<0,05) among the treatments.The conclusion of this study is adding of L-Arginin Amino Acid in skim milk diluents maintain motility, viability and membrane integrity buck's sperm.

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Mechanism of Action of L-arginine on the Vitality of Spermatozoa is Primarily Through Increased Biosynthesis of Nitric Oxide

Cited by 68 publications

References 13 publications

Efeito da inibição da óxido nítrico sintase induzível na capacitação in vitro de espermatozoides bovinos

Efeito da inibição da óxido nítrico sintase induzível na capacitação in vitro de espermatozoides bovinos

The Addition of L-Arginine in Capacitation Media to Motility, Viability, and Spermatozoa Capacity of Goats

Adding of L-Arginin Amino Acidin Skim Milk Diluent to Maintain Quality of Buck Sperm in Cold Temperature

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