Mechanism of 2,3-butanediol stereoisomers formation in a newly isolated Serratia sp. T241

Zhang, Liaoyuan; Guo, Zewang; Chen, Jiebo; Xu, Quanming; Lin, Hui; Hu, Kaihui; Guan, Xiong; Shen, Yang

doi:10.1038/srep19257

Cited by 26 publications

(39 citation statements)

References 37 publications

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“…Recent reports showed that three meso-2,3-BDHs from Klebsiella pneumoniae XJ-Li, Serratia marcescens H30, and Serratia sp. T241 could efficiently catalyze the oxidation of meso-2,3-BD into (3R)-AC [24,25]. Compared with reported (2R,3R)-2,3-BDHs, these reported meso-2,3-BDHs possessed lower K m and higher k c a t…”

Section: Introductionmentioning

confidence: 86%

“…As reported in our previous study, meso-2,3-BDH from Serratia sp. T241 could efficiently convert (2S,3S)-2,3- BD and meso-2,3-BD into (3S)-AC and (3R)-AC, respectively, whereas (2R,3R)-2,3-BD was not a suitable substrate for meso-2,3-BDH [25]. Next, E. coli/pET-mbdh-nox carrying mbdh and nox encoding meso-2,3-BDH and NOX from Serratia sp.…”

Section: Development Of the Whole-cell Biocatalystmentioning

confidence: 97%

“…The substrate and product in the reaction mixtures were determined by gas chromatography (Agilent GC9860) as described previously [25,30].…”

Section: Chiral Gc Analysismentioning

confidence: 99%

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Efficient (3R)-Acetoin Production from meso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression of meso-2,3-Butanediol Dehydrogenase, NADH Oxidase, and Vitreoscilla Hemoglobin

Guo¹,

Zhao²,

He³

et al. 2017

Journal of Microbiology and Biotechnology

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Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3)-AC and (3)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3)-AC production from -2,3-butanediol (-2,3-BD) was obtained using recombinant cells co-expressing-2,3-butanediol dehydrogenase (-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from sp. T241,, and , respectively. The new biocatalyst of/pET--- was developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/l -2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3)-AC production. The results exhibit the industrial potential for (3)-AC production via whole-cell biocatalysis.

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Section: Introductionmentioning

confidence: 86%

Section: Development Of the Whole-cell Biocatalystmentioning

confidence: 97%

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Efficient (3R)-Acetoin Production from meso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression of meso-2,3-Butanediol Dehydrogenase, NADH Oxidase, and Vitreoscilla Hemoglobin

Guo¹,

Zhao²,

He³

et al. 2017

Journal of Microbiology and Biotechnology

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“…Several specific R-BDO forming 2,3-butanediol dehydrogenases have been reported previously122526, but as mentioned in the introduction there are secondary alcohol dehydrogenases (sADH) that can carry out this transformation just as efficiently10. We used an sADH from A. xylosooxidans , which we first expressed in the wild type (VJ021 = MG1363 expressing SadB).…”

Section: Resultsmentioning

confidence: 99%

Synthesis of (3R)-acetoin and 2,3-butanediol isomers by metabolically engineered Lactococcus lactis

Kandasamy

Liu

Dantoft

et al. 2016

Sci Rep

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The potential that lies in harnessing the chemical synthesis capabilities inherent in living organisms is immense. Here we demonstrate how the biosynthetic machinery of Lactococcus lactis, can be diverted to make (3R)-acetoin and the derived 2,3-butanediol isomers meso-(2,3)-butanediol (m-BDO) and (2R,3R)-butanediol (R-BDO). Efficient production of (3R)-acetoin was accomplished using a strain where the competing lactate, acetate and ethanol forming pathways had been blocked. By introducing different alcohol dehydrogenases into this strain, either EcBDH from Enterobacter cloacae or SadB from Achromobacter xylosooxidans, it was possible to achieve high-yield production of m-BDO or R-BDO respectively. To achieve biosustainable production of these chemicals from dairy waste, we transformed the above strains with the lactose plasmid pLP712. This enabled efficient production of (3R)-acetoin, m-BDO and R-BDO from processed whey waste, with titers of 27, 51, and 32 g/L respectively. The corresponding yields obtained were 0.42, 0.47 and 0.40 g/g lactose, which is 82%, 89%, and 76% of maximum theoretical yield respectively. These results clearly demonstrate that L. lactis is an excellent choice as a cell factory for transforming lactose containing dairy waste into value added chemicals.

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“…For example, 2,3-butanediol can be used as a liquid fuel additive because of its high combustion value (27.198 Jg −1 ), as an antifreeze agent due to the low freezing point of −60 °C, as a carrier for pharmaceuticals and as a precursor in the production of methyl ethyl ketone and 1,3-butadiene through dehydration. In addition, 2,3-butanediol is easily dehydrogenated into acetoin and diacetyl, which can be used in food and cosmetics similar to flavouring agents1415. Klebsiella pneumoniae 16, a Gram-negative bacterium, is an industrially important bacterium that produces bio-based chemicals, including 2,3-butanediol and acetoin17.…”

mentioning

confidence: 99%

The metabolic flux regulation of Klebsiella pneumoniae based on quorum sensing system

Sun

Zhang

et al. 2016

Sci Rep

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Quorum-sensing (QS) systems exist universally in bacteria to regulate multiple biological functions. Klebsiella pneumoniae, an industrially important bacterium that produces bio-based chemicals such as 2,3-butanediol and acetoin, can secrete a furanosyl borate diester (AI-2) as the signalling molecule mediating a QS system, which plays a key regulatory role in the biosynthesis of secondary metabolites. In this study, the molecular regulation and metabolic functions of a QS system in K. pneumoniae were investigated. The results showed that after the disruption of AI-2-mediated QS by the knockout of luxS, the production of acetoin, ethanol and acetic acid were relatively lower in the K. pneumoniae mutant than in the wild type bacteria. However, 2,3-butanediol production was increased by 23.8% and reached 54.93 g/L. The observed enhancement may be attributed to the improvement of the catalytic activity of 2,3-butanediol dehydrogenase (BDH) in transforming acetoin to 2,3-butanediol. This possibility is consistent with the RT-PCR-verified increase in the transcriptional level of budC, which encodes BDH. These results also demonstrated that the physiological metabolism of K. pneumoniae was adversely affected by a QS system. This effect was reversed through the addition of synthetic AI-2. This study provides the basis for a QS-modulated metabolic engineering study of K. pneumoniae.

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Mechanism of 2,3-butanediol stereoisomers formation in a newly isolated Serratia sp. T241

Cited by 26 publications

References 37 publications

Efficient (3R)-Acetoin Production from meso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression of meso-2,3-Butanediol Dehydrogenase, NADH Oxidase, and Vitreoscilla Hemoglobin

Efficient (3R)-Acetoin Production from meso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression of meso-2,3-Butanediol Dehydrogenase, NADH Oxidase, and Vitreoscilla Hemoglobin

Synthesis of (3R)-acetoin and 2,3-butanediol isomers by metabolically engineered Lactococcus lactis

The metabolic flux regulation of Klebsiella pneumoniae based on quorum sensing system

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