Mechanism of 17β-estradiol degradation by Rhodococcus equi via the 4,5-seco pathway and its key genes

Tian, Kejian; Qi, Meng; Li, Shuaiguo; Chang, Menghan; Meng, Fanjuan; Yu, Yue; Li, Han; Qiu, Qing; Shao, Junhua; Huo, Hongliang

doi:10.1016/j.envpol.2022.120021

Cited by 15 publications

(5 citation statements)

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“…Therefore, purification of bacterially encoded estrogen-degrading enzymes is of great significance. Although hydroxyl dehydrogenases encoded by bacteria have also been reported, such as OecA [ 44 ], 17β-HSDx [ 36 ], 3-oxoacyl-(acyl-Carrier-protein) reductase [ 23 ] and hsd17b14 [ 7 ], but their estrogen repair capacity in wastewater has not yet been investigated to the best of our knowledge.…”

Section: Discussionmentioning

confidence: 99%

“…Scientific investigations have shown that they have adverse effects on the ecological and biological health [ 5 , 6 ]. Strong biological activity has been observed even at the level [ 7 , 8 ]. Estradiol (E2) is a typical member of the estrogen family and has the highest estrogenic activity.…”

Section: Introductionmentioning

confidence: 99%

“…Functional microbial degradation of E2 is a green and cost-effective method [ 17 , 18 ]. Currently, many E2-degrading bacteria have been isolated, which can convert E2 to substances with low or no estrogenic activity using estrogen as the only carbon source substance [ 7 , 19 , 20 ]. It is well known that the E2 biodegradation occurs mainly through enzyme catalysis.…”

Section: Introductionmentioning

confidence: 99%

“…It is well known that the E2 biodegradation occurs mainly through enzyme catalysis. In most of the reported E2-degrading bacteria, enzyme-catalyzed conversion of E2 to estrone (E1) is considered as the first reaction step in E2 degradation [ 7 , 20 – 22 ]. This step of reaction is the key and restriction step for E2 to be biodegraded [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is of great importance to explore the degrading enzymes that enable the biodegradation of E2 to E1. Hydroxysteroid dehydrogenases (HSDs) of the short-chain dehydrogenase/reductase (SDR) family are involved in redox at the E2 C17 position [ 7 , 20 ]. Although many degrading bacteria have been reported, but there have been very few studies on the characterization and application of degrading enzymes.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater

Hao

Pan

et al. 2023

Microb Cell Fact

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Background 17β-estradiol (E2) residues exhibit harmful effects both for human and animals and have got global attention of the scientific community. Microbial enzymes are considered as one of the effective strategies having great potential for removal E2 residues from the environment. However, limited literature is available on the removal of E2 from wastewater using short-chain dehydrogenase. Results In this study, 17β-estradiol degrading enzyme (17β-HSD-0095) was expressed and purified from Microbacterium sp. MZT7. The optimal pH and temperature for reaction was 7 and 40 °C, respectively. Molecular docking studies have shown that the ARG215 residue form a hydrogen bond with oxygen atom of the substrate E2. Likewise, the point mutation results have revealed that the ARG215 residue play an important role in the E2 degradation by 17β-HSD-0095. In addition, 17β-HSD-0095 could remediate E2 contamination in synthetic livestock wastewater. Conclusions These findings offer some fresh perspectives on the molecular process of E2 degradation and the creation of enzyme preparations that can degrade E2. Graphical Abstract

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