Mechanism for the transit-induced increase in colonic deoxycholic acid formation in cholesterol cholelithiasis

Thomas, Linzi A.; Veysey, Martin; Bathgate, T; King, Anna; French, Gary; Smeeton, Nigel; Murphy, Gérard M.; Dowling, R. H.

doi:10.1053/gast.2000.16495

Cited by 101 publications

(78 citation statements)

References 58 publications

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“…While the potential positive aspects of BSH activity of the probiotics have been previously discussed (10,36,37,49), other possible negative concerns about BSH activity have also been raised (11,28,51). Recently, Kurdi et al (25) proposed one possible beneficial consequence of BSH activity in bifidobacteria upon investigation of the cholic acid transport and accumulation in some intestinal Bifidobacterium strains, including two B. bifidum strains.…”

Section: Discussionmentioning

confidence: 99%

“…The enzyme responsible for this undesirable reaction, 7␣-dehydroxylase, has been found in Clostridium and Eubacterium species (8, 52) but not in lactic acid bacteria or bifidobacteria (25). Since the hydrolytic products of BSH have properties toxic to cells and can damage the membrane of mammalian cells (51), the accumulation of free bile acids within BSH-producing bacteria could counteract the toxic nature of deconjugated bile salts. On the other hand, it has yet to be determined what advantage BSH activity provides Bifidobacterium strains.…”

Section: Discussionmentioning

confidence: 99%

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Cloning and characterization of a bile salt hydrolase (bsh) from Bifidobacterium adolescentis

2005

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Biochemical characterization of the purified bile salt hydrolase (BSH) from Bifidobacterium bifidum ATCC 11863 revealed some distinct characteristics not observed in other species of Bifidobacterium. The bsh gene was cloned from B. bifidum, and the DNA flanking the bsh gene was sequenced. Comparison of the deduced amino acid sequence of the cloned gene with previously known sequences revealed high homology with BSH enzymes from several microorganisms and penicillin V amidase (PVA) of Bacillus sphaericus. The proposed active sites of PVA were highly conserved, including that of the Cys-1 residue. The importance of the SH group in the N-terminal cysteine was confirmed by substitution of Cys with chemically and structurally similar residues, Ser or Thr, both of which resulted in an inactive enzyme. The transcriptional start point of the bsh gene has been determined by primer extension analysis. Unlike Bifidobacterium longum bsh, B. bifidum bsh was transcribed as a monocistronic unit, which was confirmed by Northern blot analysis. PCR amplification with the type-specific primer set revealed the high level of sequence homology in their bsh genes within the species of B. bifidum.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of a bile salt hydrolase (bsh) from Bifidobacterium adolescentis

2005

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“…3 In IL-10-deficient mice, additional deletion of IL-6 or STAT3 resulted in hepatic steatosis and the elevation of serum alanine aminotransferase. 4 Thus, activation of IL-6/gp130/STAT3 signaling plays an important role in protecting against liver injury, whereas IL-30 inhibited interaction of IL-6 with gp130 and decreased IL-6-mediated production of IL-10 in a dose-dependent manner. 5 Does IL-30 suppress the production of IFN-c at the cellular or molecular level?…”

Section: Role Of Interleukin-30 As a Modulator Of Transcription Signamentioning

confidence: 98%

“…2 Because slow intestinal transit increases the cholelithogenic state due to hyperabsorption of cholesterol and synthesis of secondary hydrophobic bile salts such as deoxycholate, 4 …”

mentioning

confidence: 99%

Genetic study of FGF19 receptor variants in gallstone disease

2012

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“…However, current research data strongly suggest that microbial BSH function in the detoxification of bile salts increases the intestinal survival and persistence of producing strains, which in turn increases the overall beneficial effects associated with the strain [49,63]. Moreover, the bacterial genera that would most likely be used as probiotics (bifidobacteria and lactobacilli) are not capable of dehydroxylating de-conjugated bile salts [73][74][75], and thus the majority of the breakdown products of BSH activity by a probiotic strain may be precipitated and excreted in feces [76,77].…”

Section: Survival Under Conditions Simulating the Human Gi Tractmentioning

confidence: 99%

Characterization of Antibacterial Activity and Potential as Probiotic of Lactic Acid Bacteria Isolated from Goat’s Milk in Algeria

Benreguieg¹,

Dalache²,

Gacemi³

2013

JLS

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Lactic acid bacteria (LAB) were isolated from goat's milk in different north west region of Algeria. A total of 233 isolates were identified as LAB. However, only 11 strains showed excellent inhibition zones on agar when Salmonella typhimorium ATCC 13311 was used as an indicator for preliminary detection of antagonistic activity. After elimination of inhibition due to acid and H 2 O 2 , Staphylococcus aureus ATCC 25923 was used for secondary screening for antagonistic activity of these 11 strains. It was found that only 9 strains exhibited a good inhibition zones on agar, and all of them could inhibit E. coli ATCC 25921 and Pseudomonas aeruginosa ATCC 27853 as the third and the forth indicators respectively. Lactococcus lactis subsp. cremoris, Lactobacillus casei subsp. casei, Lactobacillus casei subsp. rhamnosus, Streptococcus salivarius subsp. thermophilus and Lactococcus lactis biovar.diacetylactis were the strains that have a good antibacterial activity, survive in the gastrointestinal conditions and were sensible to the majority of the antibiotics. All isolated strains tolerate pH = 2 and high bile salt concentration.

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Mechanism for the transit-induced increase in colonic deoxycholic acid formation in cholesterol cholelithiasis

Cited by 101 publications

References 58 publications

Cloning and characterization of a bile salt hydrolase (bsh) from Bifidobacterium adolescentis

Cloning and characterization of a bile salt hydrolase (bsh) from Bifidobacterium adolescentis

Genetic study of FGF19 receptor variants in gallstone disease

Characterization of Antibacterial Activity and Potential as Probiotic of Lactic Acid Bacteria Isolated from Goat’s Milk in Algeria

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