Mechanism Exploration on the Immunoregulation of Allogeneic Heart Transplantation Rejection in Rats With Exosome miRNA and Proteins From Overexpressed IDO1 BMSCs

Zheng, Rui; Wu, Xinxin; Li, Si; Chen, Xinhao; Yan, Dan; He, Jigang

doi:10.1177/09636897241245796

Cited by 2 publications

(1 citation statement)

References 70 publications

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“…Human umbilical cord-derived MSCs alleviated myocardial fibrosis and restored miRNA-133a expression in diabetic cardiomyopathy, which positively influenced fibrosis markers and inflammatory mediators in a diabetic mouse model [79]. Additionally, the immunoregulatory properties of exosomal miRNAs from bone marrow MSCs overexpressing IDO1 demonstrated their potential to modulate immune responses and improve allogeneic heart transplantation outcomes by influencing key immune-related proteins and miRNAs [80]. Zhu et al [81] reported that suppressing miR-873-5p rejuvenates aging MSCs, enhancing their functionality and therapeutic efficacy for myocardial infarction repair through modulating autophagy via the AMPK signaling pathway.…”

Section: Extracellular Vesicles and Secretomementioning

confidence: 98%

Emerging Strategies in Mesenchymal Stem Cell-Based Cardiovascular Therapeutics

Kumar,

Mishra,

Tran

et al. 2024

Cells

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Cardiovascular diseases continue to challenge global health, demanding innovative therapeutic solutions. This review delves into the transformative role of mesenchymal stem cells (MSCs) in advancing cardiovascular therapeutics. Beginning with a historical perspective, we trace the development of stem cell research related to cardiovascular diseases, highlighting foundational therapeutic approaches and the evolution of cell-based treatments. Recognizing the inherent challenges of MSC-based cardiovascular therapeutics, which range from understanding the pro-reparative activity of MSCs to tailoring patient-specific treatments, we emphasize the need to refine the pro-regenerative capacity of these cells. Crucially, our focus then shifts to the strategies of the fourth generation of cell-based therapies: leveraging the secretomic prowess of MSCs, particularly the role of extracellular vesicles; integrating biocompatible scaffolds and artificial sheets to amplify MSCs’ potential; adopting three-dimensional ex vivo propagation tailored to specific tissue niches; harnessing the promise of genetic modifications for targeted tissue repair; and institutionalizing good manufacturing practice protocols to ensure therapeutic safety and efficacy. We conclude with reflections on these advancements, envisaging a future landscape redefined by MSCs in cardiovascular regeneration. This review offers both a consolidation of our current understanding and a view toward imminent therapeutic horizons.

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Section: Extracellular Vesicles and Secretomementioning

confidence: 98%

Emerging Strategies in Mesenchymal Stem Cell-Based Cardiovascular Therapeutics

Kumar,

Mishra,

Tran

et al. 2024

Cells

View full text Add to dashboard Cite

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Exosome-related gene identification and diagnostic model construction in hepatic ischemia-reperfusion injury

You,

Chen,

Tang

et al. 2024

Sci Rep

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Hepatic ischemia-reperfusion injury (HIRI) may cause severe hepatic impairment, acute hepatic insufficiency, and multiorgan system collapse. Exosomes can alleviate HIRI. Therefore, this study explored the role of exosomal-related genes (ERGs) in HIRI using bioinformatics to determine the underlying molecular mechanisms and novel diagnostic markers for HIRI. We merged the GSE12720, GSE14951, and GSE15480 datasets obtained from the Gene Expression Omnibus (GEO) database into a combined gene dataset (CGD). CGD was used to identify differentially expressed genes (DEGs) based on a comparison of the HIRI and healthy control cohorts. The impact of these DEGs on HIRI was assessed through gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA). ERGs were retrieved from the GeneCards database and prior studies, and overlapped with the identified DEGs to yield the set of exosome-related differentially expressed genes (ERDEGs). Functional annotations and enrichment pathways of these genes were determined using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Diagnostic models for HIRI were developed using least absolute shrinkage and selection operator (LASSO) regression and support vector machine (SVM) algorithms. Key genes with diagnostic value were identified from the overlap, and single-sample gene-set enrichment analysis (ssGSEA) was conducted to evaluate the immune infiltration characteristics. A molecular regulatory interaction network was established using Cytoscape software to elucidate the intricate regulatory mechanisms of key genes in HIRI. Finally, exosome score (Es) was obtained using ssGSEA and the HIRI group was divided into the Es_High and Es_Low groups based on the median Es. Gene expression was analyzed to understand the impact of all genes in the CGD on HIRI. Finally, the relative expression levels of the five key genes in the hypoxia-reoxygenation (H/R) model were determined using quantitative real-time PCR (qRT-PCR). A total of 3810 DEGs were identified through differential expression analysis of the CGD, and 61 of these ERDEGs were screened. Based on GO and KEGG enrichment analyses, the ERDEGs were mainly enriched in wound healing, MAPK, protein kinase B signaling, and other pathways. GSEA and GSVA revealed that these genes were mainly enriched in the TP53, MAPK, TGF , JAK-STAT, MAPK, and NFKB pathways. Five key genes ( ANXA1 , HNRNPA2B1 , ICAM1 , PTEN , and THBS1 ) with diagnostic value were screened using the LASSO regression and SVM algorithms and their molecular interaction network was established using Cytoscape software. Based on ssGSEA, substantial variations were found in the expression of 18 immune cell types among the groups ( p < 0.05). Finally, the Es of each HIRI patient was calculated. ERDEGs in the Es_High and Es_Low groups were enriched in the IL18, TP53, MAPK, TGF , and J...

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Mechanism Exploration on the Immunoregulation of Allogeneic Heart Transplantation Rejection in Rats With Exosome miRNA and Proteins From Overexpressed IDO1 BMSCs

Cited by 2 publications

References 70 publications

Emerging Strategies in Mesenchymal Stem Cell-Based Cardiovascular Therapeutics

Emerging Strategies in Mesenchymal Stem Cell-Based Cardiovascular Therapeutics

Exosome-related gene identification and diagnostic model construction in hepatic ischemia-reperfusion injury

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