Mechanism behind Petal Color Mutation Induced by Heavy-Ion-Beam Irradiation of Recalcitrant Chrysanthemum Cultivar

Ohmiya, Akemi; Toyoda, Tomomi; Watanabe, H.; Emoto, Keishi; Hase, Yoshihiro; Yoshioka, Satoshi

doi:10.2503/jjshs1.81.269

Cited by 17 publications

(11 citation statements)

References 26 publications

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“…Similarly, Glick (2009) found a high correlation between carotenoid degradation in the rose cultivars “Frisco” and “Golden gate” and the expression of RhCCD4. In chrysanthemum, the loss of the CmCCD4a gene caused a change in petal color from white to yellow; the level of color mutation from white to yellow may depend on the copy number of the CmCCD4a gene (Ohmiya et al, 2012; Yoshioka et al, 2012). We detected several significant SNPs for carotenoid accumulation on Fragaria chromosome 4 and Prunus chromosome 1, close to a CCD4 gene (Figures 7A,B, Table S5).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

et al. 2016

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Petal color is one of the key characteristics determining the attractiveness and therefore the commercial value of an ornamental crop. Here, we present the first genome-wide association study for the important ornamental crop rose, focusing on the anthocyanin and carotenoid contents in petals of 96 diverse tetraploid garden rose genotypes. Cultivated roses display a vast phenotypic and genetic diversity and are therefore ideal targets for association genetics. For marker analysis, we used a recently designed Axiom SNP chip comprising 68,000 SNPs with additionally 281 SSRs, 400 AFLPs and 246 markers from candidate genes. An analysis of the structure of the rose population revealed three subpopulations with most of the genetic variation between individual genotypes rather than between clusters and with a high average proportion of heterozygous loci. The mapping of markers significantly associated with anthocyanin and carotenoid content to the related Fragaria and Prunus genomes revealed clusters of associated markers indicating five genomic regions associated with the total anthocyanin content and two large clusters associated with the carotenoid content. Among the marker clusters associated with the phenotypes, we found several candidate genes with known functions in either the anthocyanin or the carotenoid biosynthesis pathways. Among others, we identified a glutathione-S-transferase, 4CL, an auxin response factor and F3'H as candidate genes affecting anthocyanin concentration, and CCD4 and Zeaxanthine epoxidase as candidates affecting the concentration of carotenoids. These markers are starting points for future validation experiments in independent populations as well as for functional genomic studies to identify the causal factors for the observed color phenotypes. Furthermore, validated markers may be interesting tools for marker-assisted selection in commercial breeding programmes in that they provide the tools to identify superior parental combinations that combine several associated markers in higher dosages.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

et al. 2016

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“…The cultivars of chrysanthemum species have been generated through hybridization, but the floriculture industry relies on a limited number of mutated traits established based on specific flower quality parameters and consumer palatability [19]. Mutation breeding involves the use of a mutagen to develop plants exhibiting novel mutated characteristics that do not disturb elite cultivar traits [20,21]. Novel chrysanthemum cultivars generated by radiation mutagenesis and showing improved agronomic characteristics have been developed using mutation breeding techniques [7,12,21].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Phytochemical Composition of Gamma-Irradiated Mutant Cultivars of Chrysanthemum morifolium

et al. 2019

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The flowers of chrysanthemum species are used as a herbal tea and in traditional medicine. In addition, members of the genus have been selected to develop horticultural cultivars of diverse floral colors and capitulum forms. In this research, we investigated the phytochemical composition of eight gamma-irradiation mutant cultivars of Chrysanthemum morifolium and their original cultivars. The mutant chrysanthemum cultivars were generated by treatment with various doses of 60Co gamma irradiation of stem cuttings of three commercial chrysanthemum cultivars as follows: ‘ARTI-Dark Chocolate’ (50Gy), ‘ARTI-Purple Lady’ (30 Gy), and ‘ARTI-Yellow Star’ (50 Gy) derived from ‘Noble Wine’; ‘ARTI-Red Star’ (50 Gy) and ‘ARTI-Rising Sun’ (30 Gy) from ‘Pinky’; ‘ARTI-Purple’ (40 Gy) and ‘ARTI-Queen’ (30 Gy) from ‘Argus’; and ‘ARTI-Rollypop’ (70 Gy) from ‘Plaisir d’amour’. Quantitative analysis of flavonoids, phenolic acids, anthocyanins, and carotenoids in the flowers of the 12 chrysanthemum cultivars was performed using high performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (HPLC-DAD-ESIMS). Essential oils from the flowers of these cultivars were analyzed by gas chromatography–mass spectrometry (GC-MS). The mutant cultivars, ‘ARTI-Dark Chocolate’, ‘ARTI-Purple Lady’, ‘ARTI-Purple’, and ‘ARTI-Queen’ showed higher total amounts of flavonoid and phenolic acid compared with those of the respective original cultivars. The mutant cultivars, ‘ARTI-Dark Chocolate’, ‘ARTI-Purple Lady’ and ‘ARTI-Purple’, which produce purple to pink petals, contained more than two-times higher amounts of anthocyanins compared with those of their original cultivars. Of the mutant cultivars, ‘ARTI-Yellow Star’ in which petal color was changed to yellow, showed the greatest accumulation of carotenoids. Ninety-nine volatile compounds were detected, of which hydrocarbons and terpenoids were abundant in all cultivars analyzed. This is the first report that demonstrated the phytochemical analysis of novel chrysanthemum cultivars derived from C. morifolium hydrid using HPLC-DAD-ESIMS and GC-MS. These findings suggest that the selected mutant chrysanthemum cultivars show potential as a functional source of phytochemicals associated with the abundance of health-beneficial components, as well as good source for horticulture and pigment industries.

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“…For example, a single-nucleotide mutation in β-carotene hydroxylase 2 (CHYB2) caused orange fruit phenotype in pepper 9 . In Chrysanthemum morifolium, Brassica napus, and B. oleracea, the loss-of-function mutation of carotenoid cleavage dioxygenase 4 (CCD4) led to change in flower color from white to yellow 7,[10][11][12][13][14] . The mutation of pale yellow petal (PYP1) that was involved in xanthophyll ester production was responsible for pale yellow petal phenotype in tomato 8 .…”

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confidence: 99%

Fine mapping and candidate gene analysis of the white flower gene Brwf in Chinese cabbage (Brassica rapa L.)

Zhang

Chen

et al. 2020

Sci Rep

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Flower color can be applied to landscaping and identification of the purity of seeds in hybrid production. However, the molecular basis of white flower trait remains largely unknown in Brassica rapa. in this study, an f 2 population was constructed from the cross between 15S1040 (white flower) and 92S105 (yellow flower) for fine mapping of white flower genes in B. rapa. Genetic analysis indicated that white flower trait is controlled by two recessive loci, Brwf1 and Brwf2. Using InDel and SNP markers, Brwf1 was mapped to a 49.6-kb region on chromosome A01 containing 9 annotated genes, and among them, Bra013602 encodes a plastid-lipid associated protein (PAP); Brwf2 was located in a 59.3-kb interval on chromosome A09 harboring 12 annotated genes, in which Bra031539 was annotated as a carotenoid isomerase gene (CRTISO). The amino acid sequences of BrPAP and BrCRTISO were compared between two yellow-flowered and three white-flowered lines and critical amino acid mutations of BrPAP and BrCRTISO were identified between yellow-flowered and white-flowered lines. Therefore, Bra013602 and Bra031539 were predicted as potential candidates for white flower trait. Our results provide a foundation for further identification of Brwf and increase understanding of the molecular mechanisms underlying white flower formation in Chinese cabbage. Preliminary mapping of the Brwf genes. To determine the locations of genes controlling white flower Scientific RepoRtS | (2020) 10:6080 | https://doi.

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Mechanism behind Petal Color Mutation Induced by Heavy-Ion-Beam Irradiation of Recalcitrant Chrysanthemum Cultivar

Cited by 17 publications

References 26 publications

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

Genome-Wide Association Analysis of the Anthocyanin and Carotenoid Contents of Rose Petals

Comparative Analysis of Phytochemical Composition of Gamma-Irradiated Mutant Cultivars of Chrysanthemum morifolium

Fine mapping and candidate gene analysis of the white flower gene Brwf in Chinese cabbage (Brassica rapa L.)

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