The teeth and jaw perform the critical function of initiating the digestion by breaking the food into smaller sizes. Mastication is defined as the action of chewing foods. Biomechanical investigations of tooth and jaw are primarly involved with the mastication system. The masticatory system is composed of the dentition, bones, ligaments, muscles, and the temporomandibular joint (TMJ). The aim of biomechanical investigations of the masticatory system is to understand the fundamental relations between anatomy and function and, thus, either provide guidance to clinicians in existing treatment modes or help design new ones. In this article the functional anatomy, biomechanics of mastication, and the biomechanical aspects of prosthodontic treatments are presented. The fundamental definitions of force, stress, strain, and material properties are defined. The relevant material properties for the components of the masticatory system are reported. Biomechanical models and results related to the jaw open–close motions, TMJ loading, deformations of the mandible, and effect of endosseous implants are presented.