Mechanics of epidermal morphogenesis in the Drosophila pupa

Athilingam, Thamarailingam; Tiwari, Prabhat; Toyama, Yusuke; Saunders, Timothy E.

doi:10.1016/j.semcdb.2021.06.008

Cited by 18 publications

(12 citation statements)

References 56 publications

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“…The adult Drosophila abdominal epidermis develops from small islands (nests) of progenitor cells called histoblasts, which are specified during embryonic development. 16 , 17 Each abdominal hemisegment contains four nests: two located dorso-laterally (dorsal anterior and dorsal posterior), one located ventrally, and one spiracular nest located laterally. 18 , 19 During the larval period, the histoblasts are quiescent (G2 arrest).…”

Section: Introductionmentioning

confidence: 99%

ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit

et al. 2022

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Section: Introductionmentioning

confidence: 99%

ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit

et al. 2022

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Section: Discussionmentioning

confidence: 99%

“…Histoblast nests proliferate after pupariation and migrate by intercalation into the larval epithelial cells, which then extrude basally and die. The histoblast nests fuse and secrete the adult cuticle(ATHILINGAM et al 2021;DAVIS et al 2022; MANGIONE ANDMARTIN-BLANCO 2018;MICHEL AND DAHMANN 2020;NARDI et al 2018; PANZADE AND MATIS 2021;PRAT-ROJO et al 2020). Successful migration depends on Decapentaplegic (Dpp) and protrusive extensions at the leading edge of the histoblast nests(NINOV et al 2007;NINOV et al 2010).…”

mentioning

confidence: 99%

Imaginal disc growth factors areDrosophilaChitinase-like Proteins with roles in morphogenesis and CO₂response

Sustar

Strand

Zimmerman

et al. 2022

Preprint

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Chitinase-like proteins (CLPs) are members of the family 18 glycosyl hydrolases, which include chitinases and the enzymatically inactive CLPs. A mutation in the enzyme's catalytic site, conserved in vertebrates and invertebrates, allowed CLPs to evolve independently with functions that do not require chitinase activity. CLPs normally function during inflammatory responses, wound healing, and host defense, but when they persist at excessive levels at sites of chronic inflammation and in tissue-remodeling disorders, they correlate positively with disease progression and poor prognosis. However, little is known about their physiological function. Drosophila melanogaster has six CLPS, termed Imaginal disc growth factors (Idgfs), encoded by Idgf1, Idgf2, Idgf3, Idgf4, Idgf5, and Idgf6. In this study we developed tools to facilitate characterization of the physiological roles of the Idgfs by deleting each of the Idgf genes using the CRISPR/Cas9 system and assessing loss-of-function phenotypes. Using null lines, we showed that loss-of-function for all six Idgf proteins significantly lowers fertility and viability and compromises germ cell migration. We also showed that Idgfs play roles in epithelial morphogenesis, maintaining proper epithelial architecture and cell shape, regulating E-cadherin and cortical Actin, and protecting these tissues against CO2 exposure. Defining the normal molecular mechanisms of CLPS is key to understanding how deviations tip the balance from a physiological to a pathological state.

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“…4A). Previous work has demonstrated that hinge contraction in the proximal part of the wing causes tension resulting in cells becoming elongated in the wing along the P/D axis (Athilingam et al, 2021;Etournay et al, 2016) and because of this, we anticipated that a bias of division orientation might occur along this axis. However, surprisingly, we observe a small orientation bias at 45° to this axis (Fig.…”

Section: The Orientation Of Cell Divisions Might Be Biased By Tissue ...mentioning

confidence: 99%

Deep learning for rapid analysis of cell divisionsin vivoduring epithelial morphogenesis and repair

Turley

Chenchiah

Martin

et al. 2023

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Cell division is fundamental to all healthy tissue growth, as well as being rate-limiting in the tissue repair response to wounding and during cancer progression. However, the role that cell divisions play in tissue growth is a collective one, requiring the integration of many individual cell division events. It is particularly difficult to accurately detect and quantify multiple features of large numbers of cell divisions (including their spatio-temporal synchronicity and orientation), over extended periods of time. It would thus be advantageous to perform such analyses in an automated fashion, which can naturally be much enabled using Deep Learning. Hence, here we have developed a pipeline of Deep Learning Models that accurately identify dividing cells in time- lapse movies of epithelial tissues in vivo. Our pipeline also determines their axis of division orientation, as well as their shape changes before and after division. This strategy has enabled us to analyse the dynamic profile of cell divisions within the Drosophila pupal wing epithelium, both as it undergoes developmental morphogenesis, and as it repairs following laser wounding. We show that the axis of division is biased according to lines of tissue tension and that wounding triggers a synchronised (but not oriented) wave of cell divisions back from the leading edge.

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Mechanics of epidermal morphogenesis in the Drosophila pupa

Cited by 18 publications

References 56 publications

ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit

ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit

Imaginal disc growth factors areDrosophilaChitinase-like Proteins with roles in morphogenesis and CO₂response

Deep learning for rapid analysis of cell divisionsin vivoduring epithelial morphogenesis and repair

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Mechanics of epidermal morphogenesis in the Drosophila pupa

Cited by 18 publications

References 56 publications

ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit

ECM degradation in the Drosophila abdominal epidermis initiates tissue growth that ceases with rapid cell-cycle exit

Imaginal disc growth factors areDrosophilaChitinase-like Proteins with roles in morphogenesis and CO2response

Deep learning for rapid analysis of cell divisionsin vivoduring epithelial morphogenesis and repair

Contact Info

Product

Resources

About

Imaginal disc growth factors areDrosophilaChitinase-like Proteins with roles in morphogenesis and CO₂response