Mechanically robust hybrid hydrogels of photo-crosslinkable gelatin and laminin-mimetic peptide amphiphiles for neural induction

Isik, Melis; Eylem, Cemil Can; Hacıefendioǧlu, Tuǧba; Yıldırım, Erol; Sari, Buse; Nemutlu, Emirhan; Emregül, Emel; Okesola, Babatunde O.; Derkuş, Burak

doi:10.1039/d1bm01350e

Cited by 14 publications

(7 citation statements)

References 44 publications

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“…In accordance with these AFM measurements, the thiol-methacrylamide system enabled to shorten times of exposure to UV light, hence setting up an attractive basis for future 3D culture: the potential cell damage could also be drastically minimized. This fact is supported by a recent work of Isik et al , 66 who revealed severe differences in cell viability between hydrogels irradiated with UV light (23 mW cm –2 ) for 1 and 2 min.…”

Section: Resultssupporting

confidence: 70%

Tuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels for In Vitro Cell Culture Systems

Pamplona

González‐Lana

Romero

et al. 2023

ACS Appl. Polym. Mater.

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The mechanical microenvironment plays a crucial role in the evolution of colorectal cancer, a complex disease characterized by heterogeneous tumors with varying elasticity. Toward setting up distinct scenarios, herein, we describe the preparation and characterization of gelatin methacrylamide (GelMA)-based hydrogels via two different mechanisms: free-radical photopolymerization and photo-induced thiol-ene reaction. A precise stiffness modulation of covalently crosslinked scaffolds was achieved through the application of well-defined irradiation times while keeping the intensity constant. Besides, the incorporation of thiol chemistry strongly increased stiffness with low to moderate curing times. This wide range of finely tuned mechanical properties successfully covered from healthy tissue to colorectal cancer stages. Hydrogels prepared in phosphate-buffered saline or Dulbecco’s modified Eagle’s medium resulted in different mechanical and swelling properties, although a similar trend was observed for both conditions: thiol-ene systems exhibited higher stiffness and, at the same time, higher swelling capacity than free-radical photopolymerized networks. In terms of biological behavior, three of the substrates showed good cell proliferation rates according to the formation of a confluent monolayer of Caco-2 cells after 14 days of cell culture. Likewise, a characteristic apical-basal polarization of cells was observed for these three hydrogels. These results demonstrate the versatility of the presented platform of biomimetic materials as in vitro cell culture scaffolds.

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Section: Resultssupporting

confidence: 70%

Tuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels for In Vitro Cell Culture Systems

Pamplona

González‐Lana

Romero

et al. 2023

ACS Appl. Polym. Mater.

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“…Metabolomics study was conducted by gas chromatography–mass spectrometry (GC-MS) and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-qTOF-MS) as we previously reported. , Detailed protocols explaining the sample processing, method, data processing, bioinformatics and statistical analysis are provided in the SI.…”

Section: Methodsmentioning

confidence: 99%

Tuning the Cell-Adhesive Properties of Two-Component Hybrid Hydrogels to Modulate Cancer Cell Behavior, Metastasis, and Death Pathways

et al. 2022

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This work presents a polysaccharide and protein-based two-component hybrid hydrogel integrating the cell-adhesive gelatin-tyramine (G-Tyr) and nonadhesive hyaluronic acid-tyramine (HA-Tyr) through enzyme-mediated oxidative coupling reaction. The resulting HA-Tyr/G-Tyr hydrogel reflects the precise chemical and mechanical features of the cancer extracellular matrix and is able to tune cancer cell adhesion upon switching the component ratio. The cells form quasi-spheroids on HA-Tyr rich hydrogels, while they tend to form an invasive monolayer culture on G-Tyr rich hydrogels. The metastatic genotype of colorectal adenocarcinoma cells (HT-29) increases on G-Tyr rich hydrogels which is driven by the material's adhesive property, and additionally confirmed by the suppressed gene expressions of apoptosis and autophagy. On the other hand, HA-Tyr rich hydrogels lead the cells to necrotic death via oxidative stress in quasi-spheroids. This work demonstrates the ideality of HA-Tyr/G-Tyr to modulate cancer cell adhesion, which also has potential in preventing primary metastasis after onco-surgery, biomaterials-based cancer research, and drug testing.

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“…In a recent work reported by our group, the potential of a supramolecular hybrid hydrogel in neurogenic differentiation of multipotent stem cells was assessed by the metabolomics approach. 126 The hydrogel was formed by the electrostatic interaction of UV-responsive methacrylated gelatin and oppositely charged peptide amphiphiles that have a neuro-inductive peptide epitope. The hydrogel when inoculated with hMSCs and SH-SY5Y cells reconstructed the metabolomic structures of the cells and induced growth factor-free neural or neuronal specification of the cells.…”

Section: Omics Technologies For High-throughput-screening Of Cell–bio...mentioning

confidence: 99%

Omics technologies for high-throughput-screening of cell–biomaterial interactions

et al. 2022

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Mechanically robust hybrid hydrogels of photo-crosslinkable gelatin and laminin-mimetic peptide amphiphiles for neural induction

Abstract: A supramolecular hydrogel providing a biomimetic neural microenvironment with improved mechanical properties presents a laminin-derived neuro-inductive peptide epitope that induces multipotent stem cells into neural-lineage cells.

Cited by 14 publications

References 44 publications

Tuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels for In Vitro Cell Culture Systems

Tuning of Mechanical Properties in Photopolymerizable Gelatin-Based Hydrogels for In Vitro Cell Culture Systems

Tuning the Cell-Adhesive Properties of Two-Component Hybrid Hydrogels to Modulate Cancer Cell Behavior, Metastasis, and Death Pathways

Omics technologies for high-throughput-screening of cell–biomaterial interactions

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