Mechanically Robust Gels Formed from Hydrophobized Cellulose Nanocrystals

Nigmatullin, Rinat; Harniman, Robert L.; Gabrielli, Valeria; Muñoz–García, Juan C.; Khimyak, Yaroslav Z.; Angulo, Jesús; Eichhorn, Stephen J.

doi:10.1021/acsami.8b05067

Cited by 33 publications

(60 citation statements)

References 28 publications

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“…Whilst the cantilever type was kept constant between samples, fresh cantilevers were used for each to ensure the highest possible topographic resolution was obtained. When comparing adhesion interaction forces for different samples it was therefore necessary to negate possible influences of small differences in tip radius through normalising to that of the mica background consistent across all samples 43,44 . Data was analysed using Nanoscope Analysis software (Bruker).…”

Section: Methodsmentioning

confidence: 99%

Structural features distinguishing infectious ex vivo mammalian prions from non-infectious fibrillar assemblies generated in vitro

Terry

Harniman

Sells

et al. 2019

Sci Rep

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Seeded polymerisation of proteins forming amyloid fibres and their spread in tissues has been implicated in the pathogenesis of multiple neurodegenerative diseases: so called “prion-like” mechanisms. While ex vivo mammalian prions, composed of multichain assemblies of misfolded host-encoded prion protein (PrP), act as lethal infectious agents, PrP amyloid fibrils produced in vitro generally do not. The high-resolution structure of authentic infectious prions and the structural basis of prion strain diversity remain unknown. Here we use cryo-electron microscopy and atomic force microscopy to examine the structure of highly infectious PrP rods isolated from mouse brain in comparison to non-infectious recombinant PrP fibrils generated in vitro. Non-infectious recombinant PrP fibrils are 10 nm wide single fibres, with a double helical repeating substructure displaying small variations in adhesive force interactions across their width. In contrast, infectious PrP rods are 20 nm wide and contain two fibres, each with a double helical repeating substructure, separated by a central gap of 8–10 nm in width. This gap contains an irregularly structured material whose adhesive force properties are strikingly different to that of the fibres, suggestive of a distinct composition. The structure of the infectious PrP rods, which cause lethal neurodegeneration, readily differentiates them from all other protein assemblies so far characterised in other neurodegenerative diseases.

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Section: Methodsmentioning

confidence: 99%

Structural features distinguishing infectious ex vivo mammalian prions from non-infectious fibrillar assemblies generated in vitro

Terry

Harniman

Sells

et al. 2019

Sci Rep

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“…[41] However,t he emulsions stabilized at pH 7a nd 11 were not able to form an etwork by increasing the angular frequency. [51,52] Wettability and adsorption energy…”

Section: Rheological Characteristicsmentioning

confidence: 99%

Pickering/Non‐Pickering Emulsions of Nanostructured Sulfonated Lignin Derivatives

Ghavidel

Fatehi

2020

ChemSusChem

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Sulfoethylated lignin (SEKL) polymeric surfactant and sulfoethylated lignin nanoparticles (N‐SEKL) with a size of 750±50 nm are produced by using a facile green process involving a solvent‐free reaction and acidification‐based fractionation. SEKL forms a liquid‐like conventional emulsion with low viscosity that has temporary stability (5 h) at pH 7. However, N‐SEKL forms a gel‐like, motionless, and ultra‐stable Pickering emulsion through a network of interactions between N‐SEKL particles, which creates steric hindrance among the oil droplets at pH 3. The deposition of SEKL and N‐SEKL on the oil surface is monitored by a using a quartz crystal microbalance. Experimentally, the formation of emulsions at pH 7 is found to be reversible owing to the low adsorption energy ΔE of SEKL on the oil droplet (ΔE≈15 kBT), which is determined with the help of three‐phase contact‐angle measurements. However, the high desorption energy (ΔE≈6.0×105 kBT) of N‐SEKL makes it irreversibly adsorb on the oil droplets. SEKL is too hydrophilic to attach to the oil interface (ΔE≈0) and thus does not facilitate emulsion formation at pH 11. Therefore, it is feasible to apply SEKL for the formulation of Pickering or non‐Pickering emulsions in the form of nanoparticles or polymeric surfactants, depending on the targeted application.

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“…Changes to mechanical loading have been observed to change skeletal stiffness at both at the nano and micro scales (Turko et al , 2017). This led us to investigate the relative material properties of lower jaw cartilage using AFM (Melbourne et al , 2018; Nigmatullin et al , 2018, 2019; Swift et al , 2018; Terry et al , 2019; Gubała et al , 2020). Measurements were taken from areas of immature chondrocytes and hypertrophic chondrocytes (location of measurements shown by schematics in Figure 1M, N).…”

Section: Resultsmentioning

confidence: 99%

“…65,536 measurements were taken per scanned region and their RMS average recorded for comparison. Data was normalised to values from 1g static samples to show the relative YMs (Melbourne et al , 2018; Nigmatullin et al , 2018, 2019; Swift et al , 2018; Terry et al , 2019; Gubała et al , 2020).…”

Section: Methodsmentioning

confidence: 99%

Exposure to hypergravity during zebrafish development alters cartilage material properties and strain distribution

Lawrence

Aggleton

Loon

et al. 2020

Preprint

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Terrestrial vertebrates have adapted to life on Earth and its constant gravitational field, which exerts load on the body and influences the structure and function of tissues. While the effects of microgravity on muscle and bone homeostasis are well described, the effects of shorter exposures to increased gravitational fields are less well characterized. Here, we exposed zebrafish to 3 and 6g hypergravity from 3-5 days post fertilisation, when key events in jaw cartilage morphogenesis occur. We did not observe changes to growth, or morphology of cartilage or muscle. However, we observed altered mechanical properties of jaw cartilages. We model the impact of these material property changes using Finite Element Analysis and show strain distribution in the jaw is altered following hypergravity. In regions of predicted altered strain we observed local changes to chondrocyte morphology, suggesting altered gravity affects chondrocyte maturation, ultimately leading to changes to cartilage structure and function.

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Mechanically Robust Gels Formed from Hydrophobized Cellulose Nanocrystals

Cited by 33 publications

References 28 publications

Structural features distinguishing infectious ex vivo mammalian prions from non-infectious fibrillar assemblies generated in vitro

Structural features distinguishing infectious ex vivo mammalian prions from non-infectious fibrillar assemblies generated in vitro

Pickering/Non‐Pickering Emulsions of Nanostructured Sulfonated Lignin Derivatives

Exposure to hypergravity during zebrafish development alters cartilage material properties and strain distribution

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