Mechanically robust cationic cellulose nanofibril 3D scaffolds with tuneable biomimetic porosity for cell culture

Courtenay, James C.; Filgueiras, Jefferson G.; deAzevedo, Eduardo R.; Jin, Yun; Edler, Karen J.; Sharma, Ram I.; Scott, Janet L.

doi:10.1039/c8tb02482k

Cited by 24 publications

(22 citation statements)

References 72 publications

(79 reference statements)

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“…[ 328,329 ] The lack of cell adhesion and slow biodegradation have been its limitations for such applications. Research on modifying cellulose to enhance cell adhesion [ 330 ] and regulate the degradation [ 327 ] have been reported but not widely investigated. This slow degradation feature is indeed ideal for designing long‐lasting soft‐tissue fillers, as a way to improve the durability of currently used HA and collagen‐based fillers.…”

Section: Polymer Precursorsmentioning

confidence: 99%

Covalently Crosslinked Hydrogels via Step‐Growth Reactions: Crosslinking Chemistries, Polymers, and Clinical Impact

2021

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Hydrogels are an important class of biomaterials with the unique property of high‐water content in a crosslinked polymer network. In particular, chemically crosslinked hydrogels have made a great clinical impact in past years because of their desirable mechanical properties and tunability of structural and chemical properties. Various polymers and step‐growth crosslinking chemistries are harnessed for fabricating such covalently crosslinked hydrogels for translational research. However, selecting appropriate crosslinking chemistries and polymers for the intended clinical application is time‐consuming and challenging. It requires the integration of polymer chemistry knowledge with thoughtful crosslinking reaction design. This task becomes even more challenging when other factors such as the biological mechanisms of the pathology, practical administration routes, and regulatory requirements add additional constraints. In this review, key features of crosslinking chemistries and polymers commonly used for preparing translatable hydrogels are outlined and their performance in biological systems is summarized. The examples of effective polymer/crosslinking chemistry combinations that have yielded clinically approved hydrogel products are specifically highlighted. These hydrogel design parameters in the context of the regulatory process and clinical translation barriers, providing a guideline for the rational selection of polymer/crosslinking chemistry combinations to construct hydrogels with high translational potential are further considered.

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Section: Polymer Precursorsmentioning

confidence: 99%

Covalently Crosslinked Hydrogels via Step‐Growth Reactions: Crosslinking Chemistries, Polymers, and Clinical Impact

2021

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“…Thus, newly regenerated tissue cannot displace a cellulosic scaffold, which can be an advantage in tissue engineering. Cellulose materials have found applications in the regeneration of bone [15], neural [16], and cartilage [17] tissues, as well as in wound dressings [18]. The bioinertness of cellulose also meets the requirement that a scaffold material should not induce foreign body responses [19].…”

Section: Introductionmentioning

confidence: 99%

Cellulose Cryogels as Promising Materials for Biomedical Applications

Tyshkunova

Poshina

Skorik

2022

IJMS

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The availability, biocompatibility, non-toxicity, and ease of chemical modification make cellulose a promising natural polymer for the production of biomedical materials. Cryogelation is a relatively new and straightforward technique for producing porous light and super-macroporous cellulose materials. The production stages include dissolution of cellulose in an appropriate solvent, regeneration (coagulation) from the solution, removal of the excessive solvent, and then freezing. Subsequent freeze-drying preserves the micro- and nanostructures of the material formed during the regeneration and freezing steps. Various factors can affect the structure and properties of cellulose cryogels, including the cellulose origin, the dissolution parameters, the solvent type, and the temperature and rate of freezing, as well as the inclusion of different fillers. Adjustment of these parameters can change the morphology and properties of cellulose cryogels to impart the desired characteristics. This review discusses the structure of cellulose and its properties as a biomaterial, the strategies for cellulose dissolution, and the factors affecting the structure and properties of the formed cryogels. We focus on the advantages of the freeze-drying process, highlighting recent studies on the production and application of cellulose cryogels in biomedicine and the main cryogel quality characteristics. Finally, conclusions and prospects are presented regarding the application of cellulose cryogels in wound healing, in the regeneration of various tissues (e.g., damaged cartilage, bone tissue, and nerves), and in controlled-release drug delivery.

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“…The dried structures were used as aerogels with tunable pores (based on the droplet diameter) for cell culture, making use of the cationic CNFs for adsorption of biomaterials. [225] CNFs have also been modified by the adsorption of soy protein isolate [226] and acetylation [227] to stabilize emulsions of canola oil and soybean oil, respectively.…”

Section: Surface-modified Cnfmentioning

confidence: 99%

“…Cationic CNFs were shown to be effective stabilizers for cyclohexane‐in‐water emulsions with droplet diameters ≈5 µm. [ 225 ] Cyclohexane was chosen as the oil phase because it could be removed via freeze drying after the emulsion was formed. The dried structures were used as aerogels with tunable pores (based on the droplet diameter) for cell culture, making use of the cationic CNFs for adsorption of biomaterials.…”

Section: Nanocellulose In Emulsionsmentioning

confidence: 99%

Nanocellulose in Emulsions and Heterogeneous Water‐Based Polymer Systems: A Review

et al. 2020

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Mechanically robust cationic cellulose nanofibril 3D scaffolds with tuneable biomimetic porosity for cell culture

Abstract: Robust 3D modified cellulose scaffolds, with exquisite tuneable structure, in the form of foams, with meso and macro scale pores were prepared by a “bottom-up” approach.

Cited by 24 publications

References 72 publications

Covalently Crosslinked Hydrogels via Step‐Growth Reactions: Crosslinking Chemistries, Polymers, and Clinical Impact

Covalently Crosslinked Hydrogels via Step‐Growth Reactions: Crosslinking Chemistries, Polymers, and Clinical Impact

Cellulose Cryogels as Promising Materials for Biomedical Applications

Nanocellulose in Emulsions and Heterogeneous Water‐Based Polymer Systems: A Review

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